Data from: Immune priming specificity within and across generations reveals the range of pathogens affecting evolution of immunity in an insect

Dhinaut J, Chogne M, Moret Y

Date Published: February 27, 2017

DOI: http://dx.doi.org/10.5061/dryad.t2850

 

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Title Characterization of pathogen specificity and of the immune effectors associated to immune priming within and across generation in Tenebrio molitor
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Description The seven data files provided correspond to two separate sets of three successive experiments to study immune priming within and across generations in Tenebrio molitor. Each set of experiments aimed to test for specificity of resistance to different bacteria and examined the immune effectors involved in each type of immune priming. Individually primed (JAE-2016-00718-IndivPrimingSurvival) or maternally primed insects (JAE-2016-00718-TGIPSurvival) with inactivated bacteria (Priming treatment or Maternal priming treatment, respectively; Bt: Bacillus thuringiensis, Ec: Escherichia coli, Sa: Staphylococcus aureus, Se: Serratia entomophila or PBS: Phosphate Buffer Saline solution), were then infected (Infection treatment and Offspring infection treatment, respectively) with the same bacteria used for the priming treatments, but alive. Survival was recorded for 14 days post infection. Furthermore, development time of maternally primed offspring was recorded (JAE-2016-00718-TGIPLarvalDevelopment). Changes in immune parameters of T. molitor caused by individual (JAE-2016-00718-IndivPrimingImmuneEffectors) or maternal immune priming (JAE-2016-00718-TGIPImmuneEffectors) were tested by repeated measures before and 3 days after an immune challenge, with either inactivated B. thuringiensis or S. entomophila. Changes in hemocyte concentration (Hemocyte), both the activity of naturally activated phenoloxidase enzymes only (PO) and the activity of the proenzymes in addition to that of naturally activated phenoloxidase enzymes (Total-PO), and antibacterial peptide activity (ZI) were measured for each sample before and after the immune challenge. Finally, we tested survival of primed T. molitor adults (JAE-2016-00718-IndivPrimingPhagoBeads) and survival of offspring from primed mother (JAE-2016-00718-TGIPPhagoBeads) to bacterial infection, when phagocytosis was blocked by the injection of polystyrene beads (Beads injection treatment). Insects were individually or maternally primed with either B. thuringiensis (Bt) or S. entomophila (Se).
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When using this data, please cite the original publication:

Dhinaut J, Chogne M, Moret Y (2017) Immune priming specificity within and across generations reveals the range of pathogens affecting evolution of immunity in an insect. Journal of Animal Ecology, online in advance of print. http://dx.doi.org/10.1111/1365-2656.12661

Additionally, please cite the Dryad data package:

Dhinaut J, Chogne M, Moret Y (2017) Data from: Immune priming specificity within and across generations reveals the range of pathogens affecting evolution of immunity in an insect. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.t2850
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