Show simple item record Su, Jian-Qiang Ding, Long-Jun Xue, Kai Yao, Huai-Ying Quensen, John Bai, Shi-Jie Wei, Wen-Xue Wu, Jin-Shui Zhou, Jizhong Tiedje, James M. Zhu, Yong-Guan
dc.coverage.spatial China
dc.coverage.spatial Taoyuan 2014-11-20T18:10:03Z 2014-11-20T18:10:03Z 2014-11-20
dc.identifier doi:10.5061/dryad.t64s0
dc.identifier.citation Su J, Ding L, Xue K, Yao H, Quensen J, Bai S, Wei W, Wu J, Zhou J, Tiedje JM, Zhu Y (2015) Long-term balanced fertilization improves the soil microbial functional diversity in a phosphorus-limited paddy soil. Molecular Ecology 24(1): 136-150.
dc.description The influence of long-term chemical fertilization on soil microbial communities has been one of the frontier topics of agricultural and environmental sciences and is critical for linking soil microbial flora with soil functions. In this study, 16S rRNA gene pyrosequencing and a functional gene array, GeoChip 4.0, were used to investigate the shifts in microbial composition and functional gene structure in paddy soils with different fertilization treatments over a 22-year period. These included a control without fertilizers, chemical nitrogen fertilizer (N), N and phosphate (NP); N and potassium (NK), and N, P and K (NPK). Based on 16S rRNA gene data, both species evenness and key genera were affected by P fertilization. Functional gene array-based analysis revealed that long-term fertilization significantly changed the overall microbial functional structures. Chemical fertilization significantly increased the diversity and abundance of most genes involved in C, N, P, and S cycling, especially for the treatments NK and NPK. Significant correlations were found among functional gene structure and abundance, related soil enzymatic activities and rice yield, suggesting that a fertilizer-induced shift in the microbial community may accelerate the nutrient turnover in soil, which in turn influenced rice growth. The effect of N fertilization on soil microbial functional genes was mitigated by the addition of P fertilizer in this P-limited paddy soil, suggesting that balanced chemical fertilization is beneficial to the soil microbial community and its functions.
dc.relation.haspart doi:10.5061/dryad.t64s0/1
dc.relation.haspart doi:10.5061/dryad.t64s0/2
dc.relation.haspart doi:10.5061/dryad.t64s0/3
dc.relation.haspart doi:10.5061/dryad.t64s0/4
dc.relation.isreferencedby doi:10.1111/mec.13010
dc.relation.isreferencedby PMID:25410123
dc.subject long-term fertilization
dc.subject chemical fertilizer
dc.subject GeoChip
dc.subject 16S rRNA gene pyrosequencing
dc.subject soil microbial community
dc.title Data from: Long-term balanced fertilization improves the soil microbial functional diversity in a phosphorus-limited paddy soil
dc.type Article
prism.publicationName Molecular Ecology

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Title aligned sequence
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Description aligned 16S rRNA gene sequences of all samples
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Title otutable
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Description otutable of 16S rRNA gene sequences
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Title 16S analysis
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Description geochip data, chemical properties and biological properties of soil samples, R script for analysis of geochip data
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