Species interactions represent fundamental ecological processes that can have significant impacts on the evolutionary trajectories of species. However, the contribution of predator-prey interactions to genetic and phenotypic divergence within and between species remains largely unknown. In this context, predatory marine snails of the genus Conus exhibit considerable variation in venom composition, a phenomenon that may be due to the evolution of conotoxins in response to predator-prey interactions. It has been hypothesized that geographic differences in prey utilization drive the evolution of conotoxnis in cone snails and that the diversity of conotoxins is positively associated with prey diversity. To test if differences in predatory-prey interactions are associated with differences in venom composition, we sequenced and compared venom duct transcriptomes from 22 individuals of Conus miliaris from three geographic populations differing in dietary breath.
Conus_miliaris_TRI_6_14.fa
Transcript assembly for Conus miliaris. Assembly consists of 22 individuals collected from Easter Island, Guam and American Samoa. Assembly was created from 100 bp paired-end reads. Reads were first normalized then assembled with Trinity (version 20140413p1). Tissue source: venom ducts.
American_samoa_TRI_6_14.fa
Transcript assembly for 6 individuals of Conus miliaris collected from American Samoa. Assembly was created from 87,201,606 100 bp paired-end reads. Transcripts were assembled with Trinity (version 20140413p1). Tissue source: venom ducts.
Easter_Island_TRI_6_14.fa
Transcript assembly for 8 individuals of Conus miliaris collected from Easter Island. Assembly was created from 131,525,916 100-bp paired-end reads. Transcripts were assembled with Trinity (version 20140413p1). Tissue source: venom ducts.
Guam_TRI_6_14.fa
Transcript assembly for 8 individuals of Conus miliaris collected from Guam. Assembly was created from 130,566,380 100-bp paired-end reads. Transcripts were assembled with Trinity (version 20140413p1). Tissue source: venom ducts.
American_Samoa_blastx_annotation
Parsed BLASTx report for C. miliaris from American Samoa. Transcripts were seearched against local databases of non-redundant conotoxin and conotoxin signal sequences downloaded from the Conoserver database, annotated proteins from two mollusc species available on the NCBI Unigene database (Aplysia californica and Lottia gigantea), all Conus related proteins downloaded from NCBI's nr database as well as the Swiss-Prot database. Matches with an e-value < 10-5 were retained.
American_Samoa_annotation.txt
Conus_miliaris_blastx_annotation
Parsed BLASTx report for C. miliaris. Transcripts were seearched against local databases of non-redundant conotoxin and conotoxin signal sequences downloaded from the Conoserver database, annotated proteins from two mollusc species available on the NCBI Unigene database (Aplysia californica and Lottia gigantea), all Conus related proteins downloaded from NCBI's nr database as well as the Swiss-Prot database. Matches with an e-value < 10-5 were retained.
Easter_Island_blastx_annotation
Parsed BLASTx report for C. miliaris from Easter Island. Transcripts were seearched against local databases of non-redundant conotoxin and conotoxin signal sequences downloaded from the Conoserver database, annotated proteins from two mollusc species available on the NCBI Unigene database (Aplysia californica and Lottia gigantea), all Conus related proteins downloaded from NCBI's nr database as well as the Swiss-Prot database. Matches with an e-value < 10-5 were retained.
Guam_blastx_annotation
Parsed BLASTx report for C. miliaris from Guam. Transcripts were seearched against local databases of non-redundant conotoxin and conotoxin signal sequences downloaded from the Conoserver database, annotated proteins from two mollusc species available on the NCBI Unigene database (Aplysia californica and Lottia gigantea), all Conus related proteins downloaded from NCBI's nr database as well as the Swiss-Prot database. Matches with an e-value < 10-5 were retained.
Conus_miliaris_individual_expression_files.tar
Annotated tab-delimited expression count files (raw counts and FPKM values) with the number of reads mapping back to each transcript for all transcripts as well as toxin related only transcripts for all Conus miliaris individuals.
Conus_miliaris_populations_expression_files.tar
Annotated tab-delimited expression count files (raw counts and FPKM values) with the number of reads mapping back to each transcript for all transcripts as well as toxin related only transcripts for all populations (Easter Island, Guam, and American Samoa) of Conus miliaris.
Conus_miliaris_SNPs.tar
VCF file of 585,736 high-quality single nucleotide polymorphisms (SNPs) identified from 22 C. miliaris individuals from Easter Island, Guam and American Samoa.