Data from: A novel, enigmatic basal leafflower moth lineage pollinating a derived leafflower host illustrates the dynamics of host shifts, partner replacement, and apparent coadaptation in intimate mutualisms

Luo S, Yao G, Wang Z, Zhang D, Hembry DH

Date Published: November 11, 2016

DOI: http://dx.doi.org/10.5061/dryad.t9140

 

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Title Sequence_data.fasta
Downloaded 9 times
Description Novel DNA sequence data generated for this study from the species Glochidion lanceolarium (Phyllanthaceae) and Epicephala lanceolaria (Insecta: Lepidoptera: Gracillariidae). These data represent five loci: for Epicephala, mitochondrial COI, and nuclear EF1-α and ArgK; for Glochidion, nuclear ribosomal ITS and ETS. Sequences are the same as GenBank numbers KY078233-KY078291; however, GenBank numbers are not included in this file. For correspondences between sample numbers and GenBank sequences for Epicephala, please see Table 2 in the manuscript. Glochidion lanceolarium ITS sequence corresponds to GenBank number KY078253 and ETS sequence corresponds to GenBank number KY078252.
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Title Figures_3_4
Downloaded 3 times
Description Data used in generating Figures 3 and 4 in the manuscript (see Excel spreadsheet tab labels). Figure 3 data represent the number of adult Epicephala lanceolaria moths found in individual Glochidion lanceolarium fruit at three sites in Guangdong Province, China. Figure 4 data represent the number of Epicephala lanceolaria pupae with and without cocoons found when dissecting Glochidion lanceolarium fruit at two sites in Guangdong Province, China.
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Title MrBayes_Glochidion.nex.con
Downloaded 2 times
Description Phylogenetic hypothesis recovered using Bayesian inference implemented in MrBayes for Glochidion. Tip labels represent either specimen numbers or specific epithets for Glochidion species and are from the present study as well as Kawakita et al. (2004) Evolution and Hembry et al. (2013) Proc. Roy. Soc. B. Phyllanthus roseus is a very close relative to Glochidion, but is not pollinated by Epicephala (Kawakita and Kato 2009 Proc. R. Soc. B). Many tip labels follow the Supplementary Appendix in Hembry et al. (2013) Proc. R. Soc. B. This tree corresponds to Figure 5 and half of Figure 7 in the present manuscript.
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Title RAxML_Glochidion
Downloaded 2 times
Description Phylogenetic hypothesis for Glochidion recovered using maximum likelihood inference implemented in RAxML. Tip labels represent either specimen numbers or specific epithets for Glochidion species and are from the present study as well as Kawakita et al. (2004) Evolution and Hembry et al. (2013) Proc. Roy. Soc. B. Phyllanthus roseus is a very close relative to Glochidion, but is not pollinated by Epicephala (Kawakita and Kato 2009 Proc. R. Soc. B). Many tip labels follow the Supplementary Appendix in Hembry et al. (2013) Proc. R. Soc. B. This tree corresponds to Supplementary Figure 1 in the present manuscript.
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Title MrBayes_Epicephala.nex.con
Downloaded 5 times
Description Phylogenetic hypothesis for Epicephala, recovered using Bayesian inference implemented in MrBayes. Tip labels represent either specimen numbers and/or host species (in the genera Flueggea, Phyllanthus, Breynia, and Glochidion) from which specimens were reared, and are from the present study as well as Kawakita et al. (2004) Evolution, Kawakita et al. (2009) Proc. R. Soc. B, and Hembry et al. (2013) Proc. Roy. Soc. B. Many tip labels follow the Supplementary Appendix in Hembry et al. (2013) Proc. R. Soc. B. For E. lanceolaria OTUs, numbers refer to particular specimens; two-letter codes indicate collection localities (GB: Gaobangshan, LD: South China Botanical Garden, QA: Qiao Island, WT: Wutongshan); C = pupa with cocoon, NC = pupa with no cocoon. This tree corresponds to Figure 6 in the present manuscript.
Download MrBayes_Epicephala.nex.con.tre (9.69 Kb)
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Title RAxML_Epicephala
Downloaded 4 times
Description Phylogenetic hypothesis for Epicephala, recovered using maximum likelihood implemented in RAxML. Tip labels represent either specimen numbers and/or host species (in the genera Flueggea, Phyllanthus, Breynia, and Glochidion) from which specimens were reared, and are from the present study as well as Kawakita et al. (2004) Evolution, Kawakita et al. (2009) Proc. R. Soc. B, and Hembry et al. (2013) Proc. Roy. Soc. B. Many tip labels follow the Supplementary Appendix in Hembry et al. (2013) Proc. R. Soc. B. For E. lanceolaria OTUs, numbers refer to particular specimens; two-letter codes indicate collection localities (GB: Gaobangshan, LD: South China Botanical Garden, QA: Qiao Island, WT: Wutongshan); C = pupa with cocoon, NC = pupa with no cocoon. This tree corresponds to Supplementary Figure 2 in the present manuscript.
Download RAxML_Epicephala.result (6.115 Kb)
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Title Epicephala_constrained.nex.con
Downloaded 2 times
Description Phylogenetic hypothesis for Epicephala, recovered using Bayesian inference implemented in MrBayes constrained to force monophyly of all Glochidion-associated Epicephala. Tip labels represent either specimen numbers and/or host species (in the genera Flueggea, Phyllanthus, Breynia, and Glochidion) from which specimens were reared, and are from the present study as well as Kawakita et al. (2004) Evolution, Kawakita et al. (2009) Proc. R. Soc. B, and Hembry et al. (2013) Proc. Roy. Soc. B. Many tip labels follow the Supplementary Appendix in Hembry et al. (2013) Proc. R. Soc. B. For E. lanceolaria OTUs, numbers refer to particular specimens; two-letter codes indicate collection localities (GB: Gaobangshan, LD: South China Botanical Garden, QA: Qiao Island, WT: Wutongshan); C = pupa with cocoon, NC = pupa with no cocoon. This tree was used in Shimodaira-Hasegawa tests as described in the present manuscript.
Download Epicephala_constrained.nex.con.tre (58.01 Kb)
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Title Epicephala_negconstrained.nex.con
Downloaded 4 times
Description Phylogenetic hypothesis for Epicephala, recovered using Bayesian inference implemented in MrBayes constrained to force non-monophyly of Glochidion-associated Epicephala. Tip labels represent either specimen numbers and/or host species (in the genera Flueggea, Phyllanthus, Breynia, and Glochidion) from which specimens were reared, and are from the present study as well as Kawakita et al. (2004) Evolution, Kawakita et al. (2009) Proc. R. Soc. B, and Hembry et al. (2013) Proc. Roy. Soc. B. Many tip labels follow the Supplementary Appendix in Hembry et al. (2013) Proc. R. Soc. B. For E. lanceolaria OTUs, numbers refer to particular specimens; two-letter codes indicate collection localities (GB: Gaobangshan, LD: South China Botanical Garden, QA: Qiao Island, WT: Wutongshan); C = pupa with cocoon, NC = pupa with no cocoon. This tree was used in Shimodaira-Hasegawa tests as described in the present manuscript.
Download Epicephala_negconstrained.nex.con.tre (57.69 Kb)
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Title Pollination_and_oviposition
Downloaded 18 times
Description Video depicting pollination of and oviposition into flowers of Glochidion lanceolarium by a female Epicephala lanceolaria moth.
Download Pollination_and_oviposition.mp4 (20.90 Mb)
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When using this data, please cite the original publication:

Luo S, Yao G, Wang Z, Zhang D, Hembry DH (2017) A novel, enigmatic basal leafflower moth lineage pollinating a derived leafflower host illustrates the dynamics of host shifts, partner replacement, and apparent coadaptation in intimate mutualisms. The American Naturalist 189(4): 422-435. http://dx.doi.org/10.1086/690623

Additionally, please cite the Dryad data package:

Luo S, Yao G, Wang Z, Zhang D, Hembry DH (2017) Data from: A novel, enigmatic basal leafflower moth lineage pollinating a derived leafflower host illustrates the dynamics of host shifts, partner replacement, and apparent coadaptation in intimate mutualisms. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.t9140
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