Data from: Cell shape and the microenvironment regulate nuclear translocation of NF-kappaB in breast epithelial and tumor cells

Sero JE, Sailem HZ, Ardy RC, Almuttaqi H, Zhang T, Bakal C

Date Published: March 3, 2015

DOI: http://dx.doi.org/10.5061/dryad.tc5g4

 

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Title SUM159
Description Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma) File names refer to [row][column]-[field] See ReadMe file for culture and staining information.
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Title ZR75.1
Description Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma) File names refer to [row][column]-[field] See ReadMe file for culture and staining information.
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Title T47D
Description Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma) File names refer to [row][column]-[field] See ReadMe file for culture and staining information.
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Title SUM149
Description Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma) File names refer to [row][column]-[field] See ReadMe file for culture and staining information.
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Title SKBR3
Downloaded 1 time
Description Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma) File names refer to [row][column]-[field] See ReadMe file for culture and staining information.
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Title MDA-MB-453
Description Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma) File names refer to [row][column]-[field] See ReadMe file for culture and staining information.
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Title MDA-MB-231
Description Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma) File names refer to [row][column]-[field]
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Title MCF12A-no EGF
Description Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma) File names refer to [row][column]-[field]
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Title MDA-MB-157
Description Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma) File names refer to [row][column]-[field]
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Title MCF10A-no EGF
Description Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma) File names refer to [row][column]-[field]
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Title MCF7
Description Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma) File names refer to [row][column]-[field]
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Title JIMT1
Description Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma) File names refer to [row][column]-[field]
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Title hs578T
Description Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma) File names refer to [row][column]-[field]
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Download hs578T.zip (196.8 Mb)
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Title HCC1954
Description Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma) File names refer to [row][column]-[field]
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Download HCC1954.zip (130.1 Mb)
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Title HCC1143
Description Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma) File names refer to [row][column]-[field]
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Title HCC70
Description Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma) File names refer to [row][column]-[field]
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Download HCC70.zip (125.1 Mb)
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Title CAMA1
Description Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma) File names refer to [row][column]-[field]
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Download CAMA1.zip (122.1 Mb)
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Title BT474
Description Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma) File names refer to [row][column]-[field]
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Download BT474.zip (115.1 Mb)
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Title AU565
Description Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma) File names refer to [row][column]-[field]
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Download AU565.zip (143.4 Mb)
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Title Hep3B
Description Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma) File names refer to [row][column]-[field]
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Download Hep3B.zip (224.6 Mb)
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Title HeLa
Description Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma) File names refer to [row][column]-[field]
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Download HeLa.zip (198.7 Mb)
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Title AG1573 fibroblast
Description Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels: Channel 1 = DAPI (Sigma) Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen) Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma) File names refer to [row][column]-[field]
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When using this data, please cite the original publication:

Sero JE, Sailem HZ, Ardy RC, Almuttaqi H, Zhang T, Bakal C (2015) Cell shape and the microenvironment regulate nuclear translocation of NF-kappaB in breast epithelial and tumor cells. Molecular Systems Biology 11(3). http://dx.doi.org/10.15252/msb.20145644

Additionally, please cite the Dryad data package:

Sero JE, Sailem HZ, Ardy RC, Almuttaqi H, Zhang T, Bakal C (2015) Data from: Cell shape and the microenvironment regulate nuclear translocation of NF-kappaB in breast epithelial and tumor cells. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.tc5g4
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