Data from: Cell shape and the microenvironment regulate nuclear translocation of NF-kappaB in breast epithelial and tumor cells
Sero, Julia E., Institute of Cancer Research
Sailem, Heba Zuhair, Institute of Cancer Research
Ardy, Rico Chandra, Institute of Cancer Research
Almuttaqi, Hannah, Institute of Cancer Research
Zhang, Tongli, University of Oxford
Bakal, Chris, Institute of Cancer Research
Published Jan 16, 2016 on Dryad.
https://doi.org/10.5061/dryad.tc5g4
Cite this dataset
Sero, Julia E. et al. (2016). Data from: Cell shape and the microenvironment regulate nuclear translocation of NF-kappaB in breast epithelial and tumor cells [Dataset]. Dryad. https://doi.org/10.5061/dryad.tc5g4
Abstract
Although a great deal is known about the signaling events that promote nuclear translocation of NF‐κB, how cellular biophysics and the microenvironment might regulate the dynamics of this pathway is poorly understood. In this study, we used high‐content image analysis and Bayesian network modeling to ask whether cell shape and context features influence NF‐κB activation using the inherent variability present in unperturbed populations of breast tumor and non‐tumor cell lines. Cell–cell contact, cell and nuclear area, and protrusiveness all contributed to variability in NF‐κB localization in the absence and presence of TNFα. Higher levels of nuclear NF‐κB were associated with mesenchymal‐like versus epithelial‐like morphologies, and RhoA‐ROCK‐myosin II signaling was critical for mediating shape‐based differences in NF‐κB localization and oscillations. Thus, mechanical factors such as cell shape and the microenvironment can influence NF‐κB signaling and may in part explain how different phenotypic outcomes can arise from the same chemical cues.
Usage notes
SUM159
Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels:
Channel 1 = DAPI (Sigma)
Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen)
Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma)
File names refer to [row][column]-[field]
See ReadMe file for culture and staining information.
ZR75.1
Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels:
Channel 1 = DAPI (Sigma)
Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen)
Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma)
File names refer to [row][column]-[field]
See ReadMe file for culture and staining information.
T47D
Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels:
Channel 1 = DAPI (Sigma)
Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen)
Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma)
File names refer to [row][column]-[field]
See ReadMe file for culture and staining information.
SUM149
Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels:
Channel 1 = DAPI (Sigma)
Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen)
Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma)
File names refer to [row][column]-[field]
See ReadMe file for culture and staining information.
SKBR3
Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels:
Channel 1 = DAPI (Sigma)
Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen)
Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma)
File names refer to [row][column]-[field]
See ReadMe file for culture and staining information.
MDA-MB-453
Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program). Each file is a Multi-plane tiff, containing three fluorescence channels:
Channel 1 = DAPI (Sigma)
Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen)
Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma)
File names refer to [row][column]-[field]
See ReadMe file for culture and staining information.
MDA-MB-231
Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program).
Each file is a Multi-plane tiff, containing three fluorescence channels:
Channel 1 = DAPI (Sigma)
Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen)
Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma)
File names refer to [row][column]-[field]
MCF12A-no EGF
Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program).
Each file is a Multi-plane tiff, containing three fluorescence channels:
Channel 1 = DAPI (Sigma)
Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen)
Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma)
File names refer to [row][column]-[field]
MDA-MB-157
Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program).
Each file is a Multi-plane tiff, containing three fluorescence channels:
Channel 1 = DAPI (Sigma)
Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen)
Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma)
File names refer to [row][column]-[field]
MCF10A-no EGF
Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program).
Each file is a Multi-plane tiff, containing three fluorescence channels:
Channel 1 = DAPI (Sigma)
Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen)
Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma)
File names refer to [row][column]-[field]
MCF7
Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program).
Each file is a Multi-plane tiff, containing three fluorescence channels:
Channel 1 = DAPI (Sigma)
Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen)
Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma)
File names refer to [row][column]-[field]
JIMT1
Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program).
Each file is a Multi-plane tiff, containing three fluorescence channels:
Channel 1 = DAPI (Sigma)
Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen)
Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma)
File names refer to [row][column]-[field]
hs578T
Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program).
Each file is a Multi-plane tiff, containing three fluorescence channels:
Channel 1 = DAPI (Sigma)
Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen)
Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma)
File names refer to [row][column]-[field]
HCC1954
Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program).
Each file is a Multi-plane tiff, containing three fluorescence channels:
Channel 1 = DAPI (Sigma)
Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen)
Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma)
File names refer to [row][column]-[field]
HCC1143
Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program).
Each file is a Multi-plane tiff, containing three fluorescence channels:
Channel 1 = DAPI (Sigma)
Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen)
Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma)
File names refer to [row][column]-[field]
HCC70
Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program).
Each file is a Multi-plane tiff, containing three fluorescence channels:
Channel 1 = DAPI (Sigma)
Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen)
Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma)
File names refer to [row][column]-[field]
CAMA1
Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program).
Each file is a Multi-plane tiff, containing three fluorescence channels:
Channel 1 = DAPI (Sigma)
Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen)
Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma)
File names refer to [row][column]-[field]
BT474
Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program).
Each file is a Multi-plane tiff, containing three fluorescence channels:
Channel 1 = DAPI (Sigma)
Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen)
Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma)
File names refer to [row][column]-[field]
AU565
Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program).
Each file is a Multi-plane tiff, containing three fluorescence channels:
Channel 1 = DAPI (Sigma)
Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen)
Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma)
File names refer to [row][column]-[field]
Hep3B
Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program).
Each file is a Multi-plane tiff, containing three fluorescence channels:
Channel 1 = DAPI (Sigma)
Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen)
Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma)
File names refer to [row][column]-[field]
HeLa
Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program).
Each file is a Multi-plane tiff, containing three fluorescence channels:
Channel 1 = DAPI (Sigma)
Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen)
Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma)
File names refer to [row][column]-[field]
AG1573 fibroblast
Files are 16-bit tiffs (which means they will appear black if opened in Preview, Photoshop, or similar program).
Each file is a Multi-plane tiff, containing three fluorescence channels:
Channel 1 = DAPI (Sigma)
Channel 2 = NF-kappaB (anti-p65; Abcam ab16502 / Alexa-488 anti-rabbit; Invitrogen)
Channel 3 = DHE (dihydroethidium, hydroethidine; Sigma)
File names refer to [row][column]-[field]