Data from: Taxonomist’s nightmare … evolutionist’s delight: an integrative approach resolves species limits in jumping bristletails despite widespread hybridization and parthenogenesis

Dejaco T, Gassner M, Arthofer W, Schlick-Steiner BC, Steiner FM

Date Published: January 19, 2016

DOI: http://dx.doi.org/10.5061/dryad.tf7qr

 

Files in this package

Content in the Dryad Digital Repository is offered "as is." By downloading files, you agree to the Dryad Terms of Service. To the extent possible under law, the authors have waived all copyright and related or neighboring rights to this data. CC0 (opens a new window) Open Data (opens a new window)

Title Supplementary_material1_TM_dataset
Downloaded 26 times
Description Traditional morphometrics dataset
Download Supplementary_material1_TM_dataset.txt (16.01 Kb)
Details View File Details
Title Supplementary_material2_CO1_alignment
Downloaded 12 times
Description Cytochrome Oxidase 1 haplotype alignment
Download Supplementary_material2_CO1_alignment.nxs (87.58 Kb)
Details View File Details
Title Supplementary_material3_ITS2_alignment
Downloaded 15 times
Description Internal transcribed spacer 2 final alignment
Download Supplementary_material3_ITS2_alignment.nxs (49.22 Kb)
Details View File Details
Title Supplementary_material4_AFLP_matrix
Downloaded 27 times
Description Final AFLP matrix of four combined primer combinations
Download Supplementary_material4_AFLP_matrix.txt (417.0 Kb)
Details View File Details
Title List of specimens
Downloaded 23 times
Description List of specimens used in this study, including voucher IDs, sampling locality, GPS coordinates of sampling sites, GenBank accessions, and information whether an individual was included (1) in the traditional morphometrics (TM) and / or amplified fragment-length polymorphism (AFLP) dataset. The fifteen specimens of M. cryptoglacialis sp. n. that were listed in Dejaco et al. (2012) under M. glacialis are highlighted with bold letters.
Download TabS1_revised2.docx (195.7 Kb)
Details View File Details
Title TM character definitions
Downloaded 27 times
Description Definition of characters used in traditional morphometrics analysis
Download TabS2_revised.docx (18.44 Kb)
Details View File Details
Title Optimized AFLP scoring parameters
Downloaded 25 times
Description Optimized parameters used for the final scoring of the amplified fragment-length polymorphism profiles. The four dyes (FAM, HEX, NED and PET) represent four different primer combinations.
Download TabS3_revised.docx (15.55 Kb)
Details View File Details
Title Results of NewHybrids - engiadina group
Downloaded 26 times
Description Results of the hybrid test conducted in New Hybrids based on 466 amplified fragment-length polymorphism markers for the M. engiadina species group. Individuals were defined as parental species (z1 or z0) or putative hybrids (unknowns; highlighted red). Columns 4 to 9 represent six admixture classes and values are the posterior probabilities of belonging to one of these classes for each individual. P1 = parental species 1; P2 = parental species 2; F1 = first-generation hybrid; F2 = second-generation hybrid; BX1 = backcross to parental species 1; BX2 = backcross to parental species 2. (a) Machilis rubrofusca and sexual M. ticinensis as parental species and just M. engiadina as unknowns. (b) Machilis rubrofusca and sexual M. ticinensis as parental species and all other members of the M. engiadina species group as unknowns.
Download TabS4_revised.docx (89.76 Kb)
Details View File Details
Title Results of NewHybrids - helleri group
Downloaded 27 times
Description Results of the pairwise hybrid test conducted in NewHybrids based on 466 amplified fragment-length polymorphism markers for the M. helleri species group. Individuals were defined as parental species (z1 or z0) or putative hybrids (unknowns; highlighted red). Columns 4 to 9 represent six admixture classes and values are the posterior probabilities of belonging to one of these classes for each individual. P1 = parental species 1; P2 = parental species 2; F1 = first-generation hybrid; F2 = second-generation hybrid; BX1 = backcross to parental species 1; BX2 = backcross to parental species 2. (a) Machilis hrabei vs. M. helleri; note that individual #25 is likely a F2 hybrid even though no mito-nuclear discordance was found (see Discussion). (b) Machilis hrabei vs. M. lehnhoferi. (c) Machilis lehnhoferi vs. M. helleri.
Download TabS5_revised.docx (60.65 Kb)
Details View File Details
Title Optimal combinations of TM characters
Downloaded 23 times
Description Optimal combination of traditional morphometrics characters (identified by an exhaustive search) giving the highest classification rate in each species group.
Download TabS6_revised.docx (16.05 Kb)
Details View File Details
Title Reanalysis of TM characters including type specimens
Downloaded 21 times
Description Reanalysis of traditional morphometric characters for discriminating among final species hypotheses and classifying type specimens (when available). Classification results based on discriminant functions calculated from an optimal combination of characters (see Table S6 for details), separately for four species groups. We defined these groups according to ovipositor length (i.e., number of articles on gonapophysis VIII). This corresponded to the first steps in the Machilis identification key of Palissa (1964). Type specimens were not included in the training set. Classification success is given as the actual number (absolute) and the percentage (%) of individuals classified in original and leave-one-out cross-validated (LOOCV) discriminant analyses. Correct and wrong classifications are highlighted in green and red, respectively. dis = M. distincta, eng = M. engiadina, rub = M. rubrofusca, tic = M. ticinensis, tir = M. tirolensis, vag = M. vagans, spE = M. sp. E, spF = M. sp. F.
Download TabS7_revised2.docx (44.11 Kb)
Details View File Details
Title NJ trees of individual AFLP primer combinations
Downloaded 33 times
Description NJ trees for each of the four primer combinations (each represented by the four dyes FAM, HEX, NED, and PET) used to produce amplified fragment-length polymorphism profiles. Sample names include the following information: ’plate number’_’well number’_’specimen ID’_’species code’.
Download FigS1_revised.pdf (23.02 Mb)
Details View File Details
Title Reanalysis of traditional morphometric characters.
Downloaded 373 times
Description Principal component analysis (PCA) scatterplots based on optimal character combinations for each of the four species groups. Whenever available, type specimens were included and are represented by stars.
Download FigS2_revised.pdf (308.0 Kb)
Details View File Details
Title Delimitation results of bPTP algorithm
Downloaded 38 times
Description Species delimitation results of the Bayesian Poisson tree processes (bPTP) algorithm. Values on branches are posterior delimitation probabilities.
Download FigS3_revised2.pdf (685.8 Kb)
Details View File Details
Title Estimating the number of K from STRUCTURE results.
Downloaded 38 times
Description Graphical representation of Evanno’s ∆K (circles) and the mean marginal likelihood across K (diamonds) from each 10 replicated runs from K=5 to K=27.
Download FigS4_revised.pdf (59.47 Kb)
Details View File Details
Title Geographical distributions of M. helleri species group.
Downloaded 28 times
Description Geographical distributions of M. helleri species group. AT = Austria, CZ = Czech Republic, DE = Germany, IT = Italy.
Download FigS5_revised.pdf (6.056 Mb)
Details View File Details

When using this data, please cite the original publication:

Dejaco T, Gassner M, Arthofer W, Schlick-Steiner BC, Steiner FM (2016) Taxonomist’s nightmare … evolutionist’s delight: an integrative approach resolves species limits in jumping bristletails despite widespread hybridization and parthenogenesis. Systematic Biology 65(6): 947-974. http://dx.doi.org/10.1093/sysbio/syw003

Additionally, please cite the Dryad data package:

Dejaco T, Gassner M, Arthofer W, Schlick-Steiner BC, Steiner FM (2016) Data from: Taxonomist’s nightmare … evolutionist’s delight: an integrative approach resolves species limits in jumping bristletails despite widespread hybridization and parthenogenesis. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.tf7qr
Cite | Share
Download the data package citation in the following formats:
   RIS (compatible with EndNote, Reference Manager, ProCite, RefWorks)
   BibTex (compatible with BibDesk, LaTeX)

Search for data

Be part of Dryad

We encourage organizations to: