Data from: Venom-gland transcriptome and venom proteome of the Malaysian king cobra (Ophiophagus hannah)

Tan CH, Tan KY, Fung SY, Tan NH

Date Published: September 17, 2015

DOI: http://dx.doi.org/10.5061/dryad.th547

 

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Title Additional file 1_Malaysian Ophiophagus hannah transcriptome transcripts (FPKM >1)
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Description The sequenced reads were annotated and filtered in the analyses. The annotated reads were further classified into toxin, non-toxin and unidentified, together with the redundancy calculated in subsequent data sheets.
Download Additional File 1_MOh_annotations.xlsx (13.96 Mb)
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Title Malaysian Ophiophagus hannah Venom-gland Transcriptome Toxins Annotations (Updated 1/2/2015)
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Description The annotated toxins with its FPKM (Fragments Per Kilobase Of Exon Per Million Fragments Mapped) were sorted according to the protein family. The number of protein identified and the expression profiles were shown in the attached file.
Download Additional File 2_MOh_Transcriptome.xlsx (151.2 Kb)
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Title Malaysian Ophiophagus hannah 1D-SDS-PAGE-nano-ESI-LCMS/MS venom proteome
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Description The file contains the shotgun analyses results of Malaysian Ophiophagus hannah using the combination approaches of 1D-SDS-PAGE and nano-LCMS. The number of toxins identified and its relative abundance in every gel section were integrated and reported in the analyses.
Download Additional File 3_MOh_Proteome.xlsx (228.5 Kb)
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Title Sequence comparision of Malaysian Ophiophagus hannah (MOh; current study) with Balinese species (BOh; Vonk et al. [20]) and other reported O. hannah toxins deposited in public database
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Description Sequence comparision of Malaysian Ophiophagus hannah (MOh; current study) with Balinese species (BOh; Vonk et al. 2013) and other reported O. hannah toxins deposited in public database was carried out in the analyses.
Download Additional File 4_MOh_BOh_sequence comparison.xlsx (31.74 Kb)
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Title Fig.5a_3FTx_All_Jalview
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Description Primary multiple sequence alignment of Fig.5a (All MOh 3FTxs) performed with MUSCLE program using Jalview software.
Download Fig.5a_3FTx_All_Jalview.mfa (1.825 Kb)
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Title Fig.5b_3FTx_LNTX_Jalview
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Description Primary multiple sequence alignment of Fig.5b (LNTX) performed with MUSCLE program using Jalview software.
Download Fig.5b_3FTx_LNTX_Jalview.mfa (2.39 Kb)
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Title Fig.5c_3FTx_SNTX_Jalview
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Description Primary multiple sequence alignment of Fig.5c (SNTX) performed with MUSCLE program using Jalview software.
Download Fig.5c_3FTx_SNTX_Jalview.mfa (1.692 Kb)
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Title Fig.6_3FTxs_phylo_alignment_Mega6
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Description Primary multiple sequence alignment file of Fig.6 performed with MUSCLE program using Mega6 software.
Download Fig.6_3FTxs_phylo_alignment_Mega6.mas (4.123 Kb)
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Title Fig.6_3FTxs_phylo_FigTree
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Description Primary file of phylogenetic tree constructed with Mega 6 using the Maximum Likelihood method and FigTree 1.4 used to produce the phylogenetic tree figures.
Download Fig.6_3FTxs_phylo_FigTree.nwk (5.388 Kb)
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Title Fig.7_SVMP_Jalview
Downloaded 3 times
Description Primary multiple sequence alignment of Fig.7 (SVMP) performed with MUSCLE program using Jalview software.
Download Fig.7_SVMP_Jalview.mfa (15.68 Kb)
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Title Fig.8_CRISP_Jalview
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Description Primary multiple sequence alignment of Fig.8 (CRISP) performed with MUSCLE program using Jalview software.
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Title Fig.9_LAAO_Jalview
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Description Primary multiple sequence alignment of Fig.9 (LAAO) performed with MUSCLE program using Jalview software.
Download Fig.9_LAAO_Jalview.mfa (6.955 Kb)
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Title Fig.10_Vespryn_Jalview
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Description Primary multiple sequence alignment of Fig.10 (Vespryn) performed with MUSCLE program using Jalview software.
Download Fig.10_Vespryn_Jalview.mfa (3.736 Kb)
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Title Fig.11_PLA2_Jalview
Downloaded 6 times
Description Primary multiple sequence alignment of Fig.11 (PLA2) performed with MUSCLE program using Jalview software.
Download Fig.11_PLA2_Jalview.mfa (1.941 Kb)
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Title Fig.12_CVF_Jalview
Downloaded 4 times
Description Primary multiple sequence alignment of Fig.12 (CVF) performed with MUSCLE program using Jalview software.
Download Fig.12_CVF_Jalview.mfa (28.58 Kb)
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When using this data, please cite the original publication:

Tan CH, Tan KY, Fung SY, Tan NH (2015) Venom-gland transcriptome and venom proteome of the Malaysian king cobra (Ophiophagus hannah). BMC Genomics 16: 687. http://dx.doi.org/10.1186/s12864-015-1828-2

Additionally, please cite the Dryad data package:

Tan CH, Tan KY, Fung SY, Tan NH (2015) Data from: Venom-gland transcriptome and venom proteome of the Malaysian king cobra (Ophiophagus hannah). Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.th547
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