Data from: Don’t put all your eggs in one basket: a cost-effective and powerful method to optimize primer choice for rRNA environmental community analyses using the Fluidigm Access Array

Brown SP, Ferrer A, Dalling JW, Heath KD

Date Published: February 4, 2016

DOI: http://dx.doi.org/10.5061/dryad.tn6vn

 

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Title LR0Rre.LSU401.Mock.fasta
Downloaded 12 times
Description Fasta file containing repetitiveness sequences of OTUs described using the primer pair LR0R(Rehner)-nuLSU401.
Download LR0Rre.LSU401.Mock.fasta.txt (23.22 Kb)
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Title LR0R.LR3.Mock.fasta
Downloaded 4 times
Description Fasta file containing repetitiveness sequences of OTUs described using the primer pair LR0R-LR3.
Download LR0R.LR3.Mock.fasta.txt (20.38 Kb)
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Title ITS1F.ITS2.Mock.fasta
Downloaded 9 times
Description Fasta file containing repetitiveness sequences of OTUs described using the primer pair ITS1F-ITS2.
Download ITS1F.ITS2.Mock.fasta.txt (20.36 Kb)
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Title fITS7.ITS4.Mock.fasta
Downloaded 14 times
Description Fasta file containing repetitiveness sequences of OTUs described using the primer pair fITS7-ITS4.
Download fITS7.ITS4.Mock.fasta.txt (17.00 Kb)
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Title ITS3KYO2.ITS4KYO3.Mock.fasta
Downloaded 8 times
Description Fasta file containing repetitiveness sequences of OTUs described using the primer pair ITS3KYO2-ITS4KYO3.
Download ITS3KYO2.ITS4KYO3.Mock.fasta.txt (16.30 Kb)
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Title Mock.Community.fasta.Rep
Downloaded 10 times
Description Fasta File containing Sanger sequences fungal isolates used as a the Mock Community. Sequences are the rRNA region from the forward primer ITS1F and the reverse primer LR3. Label for each sequences includes river in which each culture was isolated on Coiba Island, Panama.
Download Mock.Community.fasta.Rep.txt (213.1 Kb)
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Title mothur.commands.annotated
Downloaded 294 times
Description A list of mothur commands used for these analyses along with annotations regarding what these commands are doing.
Download mothur.commands.annotated.txt (3.833 Kb)
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When using this data, please cite the original publication:

Brown SP, Ferrer A, Dalling JW, Heath KD (2016) Don’t put all your eggs in one basket: a cost-effective and powerful method to optimize primer choice for rRNA environmental community analyses using the Fluidigm Access Array. Molecular Ecology Resources 16(4): 946-956. http://dx.doi.org/10.1111/1755-0998.12507

Additionally, please cite the Dryad data package:

Brown SP, Ferrer A, Dalling JW, Heath KD (2016) Data from: Don’t put all your eggs in one basket: a cost-effective and powerful method to optimize primer choice for rRNA environmental community analyses using the Fluidigm Access Array. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.tn6vn
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