Data from: Use of a microfluidic platform to uncover basic features of energy and environmental stress responses in individual cells of Bacillus subtilis

Cabeen MT, Russell JR, Paulsson J, Losick R

Date Published: July 25, 2017

DOI: http://dx.doi.org/10.5061/dryad.v1k54

 

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Title 1_20160505_WT_40CCCP_temp
Downloaded 4 times
Description Response of wild-type cells to the onset of 40 μM CCCP. Representative MTC1801 cell lineages are shown, with the mother cells oriented toward the bottom of the frame and the feeding/waste channel toward the top of the frame. This experiment corresponds to Movie 1.
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Title 2_20160512_WT_25CCCP
Downloaded 7 times
Description Response of wild-type cells to the onset of 25 μM CCCP. Representative MTC1801 cell lineages are shown, with the mother cells oriented toward the bottom of the frame and the feeding/waste channel toward the top of the frame. This experiment corresponds to Movie 2.
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Title 3_20160527_WT_55CCCP_temp
Downloaded 8 times
Description Response of wild-type cells to the onset of 55 μM CCCP. Representative MTC1801 cell lineages are shown, with the mother cells oriented toward the bottom of the frame and the feeding/waste channel toward the top of the frame. This experiment corresponds to Movie 3.
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Title 4_20160602_WT_DMSO_temp
Downloaded 7 times
Description Response of wild-type cells to DMSO vehicle. Representative MTC1801 cell lineages are shown, with the mother cells oriented toward the bottom of the frame and the feeding/waste channel toward the top of the frame. This experiment corresponds to Movie 4.
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Title 5_20160608_del_rsbP_55CCCP_temp
Downloaded 7 times
Description Response of ∆rsbP cells to the onset of 55 μM CCCP. Representative MTC1801 cell lineages are shown, with the mother cells oriented toward the bottom of the frame and the feeding/waste channel toward the top of the frame. This experiment corresponds to Movie 5.
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Title 6_20170612_WT_60MPA_temp
Downloaded 7 times
Description Response of wild-type cells to the onset of 60 μg/ml mycophenolic acid. Representative MTC1801 cell lineages are shown, with the mother cells oriented toward the bottom of the frame and the feeding/waste channel toward the top of the frame. This experiment corresponds to Movie 6.
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Title 7_20161004_WT_1EtOH_temp
Downloaded 5 times
Description Response of wild-type cells to the onset of 1% ethanol. Representative MTC1801 cell lineages are shown, with the mother cells oriented toward the bottom of the frame and the feeding/waste channel toward the top of the frame. This experiment corresponds to Movie 7.
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Title 8_20161012_WT_2EtOH_temp
Downloaded 9 times
Description Response of wild-type cells to the onset of 2% ethanol. Representative MTC1801 cell lineages are shown, with the mother cells oriented toward the bottom of the frame and the feeding/waste channel toward the top of the frame. This experiment corresponds to Movie 8.
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Title 9_20170309_DU_2EtOH_temp
Downloaded 6 times
Description Response of ∆rsbU cells to the onset of 2% ethanol. Representative MTC1920 cell lineages are shown, with the mother cells oriented toward the bottom of the frame and the feeding/waste channel toward the top of the frame. This experiment corresponds to Movie 9.
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Title 10_20161006_WT_4EtOH_temp
Downloaded 7 times
Description Response of wild-type cells to the onset of 4% ethanol. Representative MTC1801 cell lineages are shown, with the mother cells oriented toward the bottom of the frame and the feeding/waste channel toward the top of the frame. This experiment corresponds to Movie 10.
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Title 11_20161020_RA_2EtOH_temp
Downloaded 9 times
Description Response of RsbRA-only cells to the onset of 2% ethanol. Representative MTC1761 cell lineages are shown, with the mother cells oriented toward the bottom of the frame and the feeding/waste channel toward the top of the frame. This experiment corresponds to Movie 11.
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Title 12_20160819_RC_2EtOH_temp
Downloaded 4 times
Description Response of RsbRC-only cells to the onset of 2% ethanol. Representative MTC1765 cell lineages are shown, with the mother cells oriented toward the bottom of the frame and the feeding/waste channel toward the top of the frame. This experiment corresponds to Movie 12.
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Title 13_20170104_RB_2EtOH_temp
Downloaded 5 times
Description Response of RsbRB-only cells to the onset of 2% ethanol. Representative MTC1763 cell lineages are shown, with the mother cells oriented toward the bottom of the frame and the feeding/waste channel toward the top of the frame. This experiment corresponds to Movie 13.
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Title 14_20161117_RD_2EtOH_temp
Downloaded 6 times
Description Response of RsbRD-only cells to the onset of 2% ethanol. Representative MTC1767 cell lineages are shown, with the mother cells oriented toward the bottom of the frame and the feeding/waste channel toward the top of the frame. This experiment corresponds to Movie 14.
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Title 15_20161026_RA_1EtOH_temp
Downloaded 6 times
Description Response of RsbRA-only cells to the onset of 1% ethanol. Representative MTC1761 cell lineages are shown, with the mother cells oriented toward the bottom of the frame and the feeding/waste channel toward the top of the frame. This experiment corresponds to Movie 15.
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Title 16_20160921_RC_1EtOH_temp
Downloaded 6 times
Description Response of RsbRC-only cells to the onset of 1% ethanol. Representative MTC1765 cell lineages are shown, with the mother cells oriented toward the bottom of the frame and the feeding/waste channel toward the top of the frame. This experiment corresponds to Movie 16.
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Title 17_20160915_RC_4EtOH_temp
Downloaded 3 times
Description Response of RsbRC-only cells to the onset of 4% ethanol. Representative MTC1765 cell lineages are shown, with the mother cells oriented toward the bottom of the frame and the feeding/waste channel toward the top of the frame. This experiment corresponds to Movie 17.
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Title 18_20161017_RA_4EtOH_temp
Downloaded 3 times
Description Response of RsbRA-only cells to the onset of 4% ethanol. Representative MTC1761 cell lineages are shown, with the mother cells oriented toward the bottom of the frame and the feeding/waste channel toward the top of the frame. This experiment corresponds to Movie 18.
Download 18_20161017_RA_4EtOH.tar (6.448 Gb)
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Title 19_20160822_DUP_LB_temp
Downloaded 5 times
Description Response of ∆rsbP ∆rsbU cells. Representative MTC1930 cell lineages are shown, with the mother cells oriented toward the bottom of the frame and the feeding/waste channel toward the top of the frame. The cells are kept in LB with no medium switch to assess whether there is any σB activation in the absence of both known activating phosphatases. This experiment corresponds to Figure S10.
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When using this data, please cite the original publication:

Cabeen MT, Russell JR, Paulsson J, Losick R. Use of a microfluidic platform to uncover basic features of energy and environmental stress responses in individual cells of Bacillus subtilis. PLOS Genetics, online in advance of print. http://dx.doi.org/10.1371/journal.pgen.1006901

Additionally, please cite the Dryad data package:

Cabeen MT, Russell JR, Paulsson J, Losick R (2017) Data from: Use of a microfluidic platform to uncover basic features of energy and environmental stress responses in individual cells of Bacillus subtilis. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.v1k54
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