Data from: Positional cloning of rp2 QTL associates the P450 genes CYP6Z1, CYP6Z3 and CYP6M7 with pyrethroid resistance in the malaria vector Anopheles funestus

 

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Title Full sequence of rp2 BAC 23 07 12
Downloaded 14 times
Description Full length sequence of the 113kb BAC clone spanning the rp2 QTL. This sequence was assembled from 6 contigs. It contains 15 P450 genes and the 1 salivary protein and is located on chromosome 2L.
Download Full sequence of rp2 BAC 23 07 12.fas (116.8 Kb)
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Title Genotype file for Family 1 and 10 for QTL mapping
Downloaded 17 times
Description Genotypes for different genetic markers in the two isofemale families (1 and 10) used for genetic and QTL mapping. Resistant individuals are coded with "1" while susceptible are coded with "0".
Download Genotype file for Family 1 and 10 for QTL...ng.Loc (16.77 Kb)
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Title DNA sequences for SNP identification 23 07 12
Downloaded 19 times
Description DNA sequences from direct sequencing of different genes in F1 samples of the isofemale families Fam1 and Fam10. These sequences were used for SNP identification and genetic variability analysis using DnaSP program.
Download DNA sequences for SNP identification 23 07 12.fas (150.3 Kb)
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Title CYP6Z1 cloned full length for FG FZ 23 07 12
Downloaded 18 times
Description Full length sequences of the P450 gene CYP6Z1 between resistant (FZ for FUMOZ-R) and susceptible (FG for FANG). These sequences were obtained after cloning.
Download CYP6Z1 cloned full length for FG FZ 23 07 12.fas (12.52 Kb)
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Title CYP6Z3 cloned full length for FG FZ 23 07 12
Downloaded 17 times
Description Full length sequences of the P450 gene CYP6Z3 between resistant (FZ for FUMOZ-R) and susceptible (FG for FANG). These sequences were obtained after cloning.
Download CYP6Z3 cloned full length for FG FZ 23 07 12.fas (17.22 Kb)
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Title qRTPCR data and PCR efficiencies
Downloaded 49 times
Description qRT-PCR Ct values and PCR efficiencies for the genes tested between the resistant FUMOZ-R and the susceptible FANG strain.
Download qRTPCR data and PCR efficiencies.xlsx (12.03 Kb)
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When using this data, please cite the original publication:

Irving H, Riveron JM, Ibrahim SS, Lobo NF, Wondji CS (2012) Positional cloning of rp2 QTL associates the P450 genes CYP6Z1, CYP6Z3 and CYP6M7 with pyrethroid resistance in the malaria vector Anopheles funestus. Heredity 109: 383–392. http://dx.doi.org/10.1038/hdy.2012.53

Additionally, please cite the Dryad data package:

Irving H, Riveron JM, Ibrahim SS, Lobo NF, Wondji CS (2012) Data from: Positional cloning of rp2 QTL associates the P450 genes CYP6Z1, CYP6Z3 and CYP6M7 with pyrethroid resistance in the malaria vector Anopheles funestus. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.v218g
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