Data from: Structural reorganization of the chromatin remodeling enzyme Chd1 upon engagement with nucleosomes

Sundaramoorthy R, Hughes AL, Singh V, Wiechens N, Ryan DP, El-Mkami H, Petoukhov M, Svergun DI, Treutlein B, Quack S, Fischer M, Michaelis J, Böttcher B, Norman DG, Owen-Hughes T

Date Published: May 4, 2017

DOI: http://dx.doi.org/10.5061/dryad.v5n53

 

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Title RAW PELDOR DATA
Downloaded 6 times
Description This is a Zipped folder containing raw data for PELDOR measurements reported in the associated publication. Within the folder are directories named with the pair of labeling sites (residues in the Chd1 protein) used for each PELDOR measurement. The data is in the format of a .DSC and .DAT files. These represent the echo signal from the paired spin interaction. The data can be opened with the DeerAnalysis software available at http://www.epr.ethz.ch/software.html .
Download RAWPELDORDATA.zip (132.9 Kb)
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Title PELDORMODEL1
Downloaded 5 times
Description This file includes the co-ordinates for the solution structure of the Chd1 protein determined from PELDOR measurements as described in the associated publication. This is a pymol session file that can be opened with the program Pymol https://www.pymol.org/ .
Download PELDORMODEL1.pse (16.22 Mb)
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Title Chd1-apo
Downloaded 9 times
Description These are the picked particle stacks used to generate the structure of the apo complex (Figure 6). http://www.pdbe.org/emd-3517 . The file can be processed using RELION as described in the manuscript.
Download Chd1-Native.tgz (3.081 Gb)
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Title Chd1-engaged
Downloaded 13 times
Description These are the motion corrected picked particle stacks used for processing the Chd1-nucleosome complex in the engaged conformation (Figure 7). http://www.pdbe.org/emd-3502. The file can be opened and processed as described in the manuscript using RELION.
Download Chd1-NEG.tgz (32.13 Gb)
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When using this data, please cite the original publication:

Sundaramoorthy R, Hughes AL, Singh V, Wiechens N, Ryan DP, El-Mkami H, Petoukhov M, Svergun DI, Treutlein B, Quack S, Fischer M, Michaelis J, Böttcher B, Norman DG, Owen-Hughes T (2017) Structural reorganization of the chromatin remodeling enzyme Chd1 upon engagement with nucleosomes. eLife 6: e22510. http://dx.doi.org/10.7554/eLife.22510

Additionally, please cite the Dryad data package:

Sundaramoorthy R, Hughes AL, Singh V, Wiechens N, Ryan DP, El-Mkami H, Petoukhov M, Svergun DI, Treutlein B, Quack S, Fischer M, Michaelis J, Böttcher B, Norman DG, Owen-Hughes T (2017) Data from: Structural reorganization of the chromatin remodeling enzyme Chd1 upon engagement with nucleosomes. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.v5n53
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