Skip to main content
Dryad

Data from: Two subunits of human ORC are dispensable for DNA replication and proliferation

Shibata, Etsuko, University of Virginia
Kiran, Manjari, University of Virginia
Shibata, Yoshiyuki, University of Virginia
Singh, Samarendra, University of Virginia
Kiran, Shashi, University of Virginia
Dutta, Anindya, University of Virginia
Published Dec 13, 2016 on Dryad. https://doi.org/10.5061/dryad.v84c2

Cite this dataset

Shibata, Etsuko et al. (2016). Data from: Two subunits of human ORC are dispensable for DNA replication and proliferation [Dataset]. Dryad. https://doi.org/10.5061/dryad.v84c2

Abstract

The six-subunit Origin Recognition Complex (ORC) is believed to be an essential eukaryotic ATPase that binds to origins of replication as a ring-shaped heterohexamer to load MCM2-7 and initiate DNA replication. We have discovered that human cell lines in culture proliferate with intact chromosomal origins of replication after disruption of both alleles of ORC2 or of the ATPase subunit, ORC1. The ORC1 or ORC2-depleted cells replicate with decreased chromatin loading of MCM2-7 and become critically dependent on another ATPase, CDC6, for survival and DNA replication. Thus, either the ORC ring lacking a subunit, even its ATPase subunit, can load enough MCM2-7 in partnership with CDC6 to initiate DNA replication, or cells have an ORC-independent, CDC6-dependent mechanism to load MCM2-7 on origins of replication

Usage notes

BrIP seq peaks

BrIP seq peaks in WT and ORC mutant cell lines
BrIP peaks.zip

BrIP seq genomic reads

BrdU incorporated genomic strand reads
Control.fastq.gz

BrIP seq WT reads

BrdU incorporated nascent strand reads in WT cells
WT_A.fastq.gz

BrIP seq ORC1B14 reads

BrdU incorporated nascent strand reads in ORC1B14 cells
ORC1.fastq.gz

BrIP seq ORC2B2 reads

BrdU incorporated nascent strand reads in ORC2B2 cells
ORC2.fastq.gz