Data from: Use of ancient sedimentary DNA as a novel conservation tool for high-altitude tropical biodiversity

 

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Title Afro-alpine taxonomic reference library version 1.0
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Description This file contains the sequences of the taxonomic reference library version 1.0 for the African alpine flora. Sequences cover the P6 loop of the trnL. Header lines contain the species names followed by identity numbers (O-DP numbers) that correspond to specimen numbers in the herbarium of the Natural History Museum, University of Oslo, Norway. Sampling for the afro-alpine taxonomic reference library was carried out during 2007-2009 in Ethiopia, Kenya, Tanzania, and Uganda. Details on DNA extractions, PCRs, sequencing, and formatting of the final database can be found in the associated publication. Contact author: Sanne Boessenkool (sanneboessenkool@gmail.com)
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Title Sequences from dataset 1 with identification using the Afro-alpine reference library
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Description The unique P6 loop trnL sequences produced by amplification of DNA preserved in sediments from the crater swamp and crater lake on Mt. Gahinga and Mt. Muhavura, respectively, on the Virunga Volcanoes of the Albertine Rift, eastern Africa. Taxonomic identification of sequences was inferred using the Afro-alpine reference library v.1. Further details can be found in the ReadMe file.
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Title Sequences from dataset 1 with identification using embl
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Description The unique P6 loop trnL sequences produced by amplification of DNA preserved in sediments from the crater swamp and crater lake on Mt. Gahinga and Mt. Muhavura, respectively, on the Virunga Volcanoes of the Albertine Rift, eastern Africa. Taxonomic identification of sequences was inferred using a reference library formatted from embl. Further details can be found in the ReadMe file.
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Title Sequences from dataset 2 with identification using the Afro-alpine reference library
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Description The unique P6 loop trnL sequences produced by amplification of DNA preserved in sediments from the crater swamp and crater lake on Mt. Gahinga and Mt. Muhavura, respectively, on the Virunga Volcanoes of the Albertine Rift, eastern Africa. Taxonomic identification of sequences was inferred using the Afro-alpine reference library v.1. Further details can be found in the ReadMe file.
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Title Sequences from dataset 2 with identification using embl
Downloaded 1 time
Description The unique P6 loop trnL sequences produced by amplification of DNA preserved in sediments from the crater swamp and crater lake on Mt. Gahinga and Mt. Muhavura, respectively, on the Virunga Volcanoes of the Albertine Rift, eastern Africa. Taxonomic identification of sequences was inferred using a reference library formatted from embl. Further details can be found in the ReadMe file.
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When using this data, please cite the original publication:

Boessenkool S, McGlynn G, Epp LS, Taylor D, Pimentel M, Gizaw A, Nemomissa S, Brochmann C, Popp M (2013) Use of ancient sedimentary DNA as a novel conservation tool for high-altitude tropical biodiversity. Conservation Biology 2(28): 446-455. http://dx.doi.org/10.1111/cobi.12195

Additionally, please cite the Dryad data package:

Boessenkool S, McGlynn G, Epp LS, Taylor D, Pimentel M, Gizaw A, Nemomissa S, Brochmann C, Popp M (2013) Data from: Use of ancient sedimentary DNA as a novel conservation tool for high-altitude tropical biodiversity. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.vg7b1
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