Skip to main content
Dryad

Data from: MR1-restricted mucosal-associated invariant T (MAIT) cells respond to mycobacterial vaccination and infection in nonhuman primates

Greene, Justin M., Oregon National Primate Research Center
Dash, Pradyot
Roy, Sobhan
McMurtrey, Curtis, University of Oklahoma Health Sciences Center
Awad, Walid, St. Jude Children's Research Hospital
Reed, Jason S., Oregon National Primate Research Center
Hammond, Katherine B., Oregon National Primate Research Center
Abdulhaqq, Shaheed, Oregon National Primate Research Center
Wu, Helen L., Oregon National Primate Research Center
Burwitz, Benjamin J., Oregon National Primate Research Center
Roth, Benjamin F., Oregon National Primate Research Center
Morrow, David W., Oregon National Primate Research Center
Ford, Julia C., Oregon National Primate Research Center
Xu, Guangwu
Bae, Joseph Y., Oregon National Primate Research Center
Crank, Hugh, Oregon National Primate Research Center
Legasse, Alfred W., Oregon National Primate Research Center
Dang, Thurston H., UNSW Sydney
Greenaway, Hui Yee, UNSW Sydney
Kurniawan, Monica, UNSW Sydney
Gold, Marielle C., Oregon Health & Science University
Harriff, Melanie J., Oregon Health & Science University
Lewinsohn, Deborah A.
Park, Byung S., Oregon National Primate Research Center
Axthelm, Michael K., Oregon National Primate Research Center
Stanton, Jeffrey J., Oregon National Primate Research Center
Hansen, Scott G., Oregon National Primate Research Center
Picker, Louis J., Oregon National Primate Research Center
Venturi, Vanessa, UNSW Sydney
Hildebrand, William, University of Oklahoma Health Sciences Center
Thomas, Paul G., St. Jude Children's Research Hospital
Lewinsohn, David M., Oregon Health & Science University
Adams, Erin J., University of Chicago
Sacha, Jonah B., Oregon National Primate Research Center
Published Sep 07, 2017 on Dryad. https://doi.org/10.5061/dryad.vm36c

Cite this dataset

Greene, Justin M. et al. (2017). Data from: MR1-restricted mucosal-associated invariant T (MAIT) cells respond to mycobacterial vaccination and infection in nonhuman primates [Dataset]. Dryad. https://doi.org/10.5061/dryad.vm36c

Abstract

Studies on mucosal-associated invariant T cells (MAITs) in nonhuman primates (NHP), a physiologically relevant model of human immunity, are handicapped due to a lack of macaque MAIT-specific reagents. Here we show that while MR1 ligand-contact residues are conserved between human and multiple NHP species, three T-cell receptor contact-residue mutations in NHP MR1 diminish binding of human MR1 tetramers to macaque MAITs. Construction of naturally loaded macaque MR1 tetramers facilitated identification and characterization of macaque MR1-binding ligands and MAITs, both of which mirrored their human counterparts. Using the macaque MR1 tetramer we show that NHP MAITs activated in vivo in response to both Bacillus Calmette-Guerin vaccination and Mycobacterium tuberculosis infection. These results demonstrate that NHP and human MR1 and MAITs function analogously, and establish a preclinical animal model to test MAIT-targeted vaccines and therapeutics for human infectious and autoimmune disease.

Usage notes

Rhesus Macaque TRAV and TRDV genes

TRAV and TRDV genes extracted from the rhesus macaque genome. The gene features reported include L-PART1, V-INTRON, V-EXON, V-HEPTAMER, V-SPACER, and V-NONAMER. The FASTA header fields include the species name, rhesus macaque chromosome 7 accession number (NC_007864.1), start and end positions of the gene feature in the rhesus macaque chromosome 7, strand (-1 indicates that the reported sequences are the reverse complement), gene name, and gene feature.
RhesusMacaque_TRAV_TRDV.fsa

Rhesus Macaque TRAJ genes

TRAJ genes extracted from the rhesus macaque genome. The gene features reported include J-NONAMER, J-SPACER, and J-HEPTAMER. The FASTA header fields include the species name, rhesus macaque chromosome 7 accession number (NC_007864.1), start and end positions of the gene feature in the rhesus macaque chromosome 7, strand (-1 indicates that the reported sequences are the reverse complement), gene name, and gene feature.
RhesusMacaque_TRAJ.fsa