Data from: Ocean acidification during pre-fertilization chemical communication affects sperm success
Cite this dataset
Lymbery, Rowan; Kennington, Jason; Cornwall, Christopher; Evans, Jonathan (2019). Data from: Ocean acidification during pre-fertilization chemical communication affects sperm success [Dataset]. Dryad. https://doi.org/10.5061/dryad.37pvmcvf2
Abstract
Methods
These data contain fertilization success scores from gametes of the mussel Mytilus galloprovincialis, following sperm chemotaxis trials in two different seawater pH treatments (ambinet and experimentally lowered pH). They also contain seawater carbonate chemostry measurements (pH on the total scale and total alkalinity) from each batch of seawater at each treatment.
Usage notes
The first spreadsheet contains fertilization success scores following sperm chemotaxis trials at the two pH treatments. Columns included are experimental block number, focal male (sperm donor) ID, pH treatment in chemotaxis chamber, fertilization design (unstandardised or standardised pH conditions in the fertilization pools - see manuscript for full description), focal male fertilization success, focal male failures (eggs not fertilized), and total eggs counted. The second spreadsheet contains seawater carbonate chemistry measurements from the seawater batches in each experimental block. The columns include sample ID (block and pH treatment), temperature, salinity, pH on the total scale (measured potentiometrically via calibration against Tris buffers) and total alkalinity (measured via potentiometric titration).
Funding
Australian Research Council, Award: DP170103290