Neurovascular coupling (NVC) is a mechanism that, amongst other known and latent critical functions, ensures activated brain regions are adequately supplied with oxygen and glucose. This biological phenomenon underpins non-invasive perfusion-related neuroimaging techniques, and recent reports have implicated NVC impairment in several neurodegenerative disorders. Yet, much remains unknown regarding NVC in health and disease, and only recently has there been burgeoning recognition of a close interplay with brain thermodynamics. Accordingly, we developed a novel multi-modal approach to systematically modulate cortical temperature and interrogate the spatiotemporal dynamics of sensory-evoked NVC. We show that changes in cortical temperature profoundly and intricately modulate NVC, with low temperatures associated with diminished oxygen delivery, and high temperatures inducing a distinct vascular oscillation. These observations provide novel insights into the relationship between NVC and brain thermodynamics, with important implications for brain-temperature-related therapies, functional biomarkers of elevated brain temperature, and in-vivo methods to study neurovascular coupling.

Optical imaging data were collected using a bespoke imaging system collecting raw data at 4 different wavelengths. This data was then subject to spectral analysis (described in the methods) to generate images over time for total blood volume (Hbt), oxyhemoglobin (Hbo) and deoxyhemoglobin (Hbr). Three selected regions were taken to generate time series data that is stored in this repository. Region 1 is the response from the whole whisker region, region two within the whisker region but close to where the middle cerebral artery emerges into the cranial window (where cooling will have the least effect) and region 3 again from within the whisker region but at its most distal portion where cooling will be most pronounced. 

The neural data consists of raw field potential data taken from 16 electrodes at a sampling frequency of 24Khz. It also contains processed Multi-unit data from these 16 electrodes representing firing of action potentials over time. 

Cortical temperature and tissue oxygen time series are also included.

All data included is across a range of cortical brain temperatures described in the manuscript.

All data has been saved in Matlab format.