Beta diversity of aquatic macroinvertebrate assemblages associated with leaf patches in neotropical montane streams
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Jan 12, 2022 version files 61.64 KB
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Abstract
In a 100-m long reach of each of the 9 stream sites, 2 sampling stations were set where we collected as targeted samples, with a Surber sampler (30 x 30 cm; 250 μm mesh), 5 leaf patches in riffles and 5 in pools in both dry (July 2006) and rainy (March 2007) seasons, totaling 180 samples. In pool habitats, we used a Surber sampler simply to ensure the same collection area in pools as riffles, placed leaf deposits into the net by hand and transferred the net contents into plastic bags for subsequent processing. Leaf patches were chosen because, as Ligeiro et al. (2020) reported, they concentrate high abundance and diversity of macroinvertebrates, acting as biodiversity hotbeds. Consequently, they limit assemblage responses to the disturbance gradient when this substrate is targeted, as opposed to using multihabitat sampling. At the sites, leaves were washed with stream water over a sieve (mesh size: 250 μm), separated manually and then the macroinvertebrates and fine particulate organic matter were placed in plastic jars and fixed with 70% ethanol. In the laboratory, invertebrates were identified to family using taxonomic keys (Pérez, 1988; Merritt & Cummins, 1996; Fernández & Domínguez, 2001; Costa et al., 2006; Mugnai et al., 2010).