Data from: Quantitative trait transcripts for nicotine resistance in Drosophila melanogaster
Data files
Oct 28, 2009 version files 78.30 MB
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Drug effect.xls
2.67 MB
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Expression on Metabolite.xls
1.49 MB
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Expression on Survival.xls
858.62 KB
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GHHDG_Supplementary.doc
410.11 KB
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Gurgel LSmeans.xls
17.90 MB
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Gurgel MIAME compliance.doc
31.23 KB
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Nic array annotation.xls
968.19 KB
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Nic raw arrays.xls
53.95 MB
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SAS scripts.doc
25.60 KB
Oct 28, 2009 version files 156.59 MB
-
Drug effect.xls
2.67 MB
-
Expression on Metabolite.xls
1.49 MB
-
Expression on Survival.xls
858.62 KB
-
GHHDG_Supplementary.doc
410.11 KB
-
Gurgel LSmeans.xls
17.90 MB
-
Gurgel MIAME compliance.doc
31.23 KB
-
Nic array annotation.xls
968.19 KB
-
Nic raw arrays.xls
53.95 MB
-
SAS scripts.doc
25.60 KB
Abstract
Although most genetic association studies are performed with the intention of detecting nucleotide polymorphisms that are correlated with a complex trait, transcript abundance should also be expected to associate with diseases or phenotypes. We performed a scan for such quantitative trait transcripts in adult female heads of the fruit fly (Drosophila melanogaster) that might explain variation for nicotine resistance. The strongest association was seen for abundance of ornithine aminotransferase transcripts, implicating detoxification and neurotransmitter biosynthesis as mediators of the quantitative response to the drug. Subsequently, genetic analysis and metabolite profiling confirmed a complex role for ornithine and GABA levels in modification of survival time upon chronic nicotine exposure. Differences between populations from North Carolina and California suggest that the resistance mechanism may be an evolved response to environmental exposure.