Pan, X., Heimburger, B., Chen, W., Lu, Z., Cordes, P. H., Xie, Z., Sun, X., Liu, D., Wu, D., Scheu, S., Schaefer, I., & Maraun, M. (2025). Older Lineages of Oribatid Mites in Mountain Ranges Have Broader Geographic Ranges and Exhibit More Generalistic Traits. Ecology and Evolution, 15(3), e71046. https://doi.org/10.1002/ece3.71046

Published Article Link:  https://doi.org/10.1002/ece3.71046  OR  https://onlinelibrary.wiley.com/doi/10.1002/ece3.71046

Description of the data and file structure

We have submitted all our related documents including supplementary files (* 7), Code (* 1), and raw data (* 6).

Dryad Dataset Link: https://doi.org/10.5061/dryad.2bvq83bz0

Raw Data

1 —– “Data___for_annotated_tree.csv” ===== This file is for statistical analyses. It includes species name, taxonomic assignment, node age, sampling sites and their traits (i.e. reproductive mode, trophic variation and distribution), which were used to map them into the phylogenetic tree. Specifically, variables from column A to column K are mountain, overlapped, infraorder, family, genus, species, species_name_in_bigtree_logfile, node_age, reproductive_mode, trophic_variation, distribution_area.

Subías, L. S. 2022a. “Listado sistemático, sinonímico y biogeográfico de los ácaros oribátidos (Acariformes, Oribatida) del mundo (excepto fósiles) (17a actualización).”

2 —– “Data___range_size.csv” ===== This file is for statistical analyses. It includes species name, taxonomic assignment, sampling sites and their range size (km-2) data. Specifically, variables from column A to column H are mountain, overlapped, infraorder, family, genus, species, species_name_in_bigtree_logfile, and distribution_area. Row 2 to row 29 is for the Alps, and row 30 to row 49 is for Changbai Mountain.

Subías, L. S. 2022a. “Listado sistemático, sinonímico y biogeográfico de los ácaros oribátidos (Acariformes, Oribatida) del mundo (excepto fósiles) (17a actualización).”

3 —– “Data___trophic_variation.csv” ===== This file is for statistical analyses. It includes species name, taxonomic assignment, sampling sites and their trophic data (15N). Specifically, variables from column A to column I are mountain, overlapped, infraorder, family, genus, species, mean_D15N, sd_D15N and number_of_measurments. Row 2 to row 28 is for the Alps, and row 29 to row 46 is for Changbai Mountain. This data only has 37 species not 40 species since 3 species lack of their trophic data: “Carabodes_coriaceus” from Alps were include in the phylogenetic tree but we don’t have its trophic data; “Hypochthonius_rufulus” and “Punctoribates_punctum” from Changbai were include in the phylogenetic tree but we don’t have their trophic data.

Subías, L. S. 2022a. “Listado sistemático, sinonímico y biogeográfico de los ácaros oribátidos (Acariformes, Oribatida) del mundo (excepto fósiles) (17a actualización).”

4 —– “14_species_18S_consensus_uploaded_to_NCBI.fasta”  ===== Species sequences generated from this study. Readers can also download them from NCBI GenBank using accession number OR820193– OR820206.

5 —– “76and3_BEAST_Run06-Tree.nex” ===== The chronogram of oribatid mite species from the Eurasian continent (76 species) generated in BEAST v2.7.5.

6 —– “76and3_MAFFT_align_Gblocks_withgaps.fasta” ===== The final alignment file which was used to build the phylogenetic tree afterwards. It contain 76 sequences of oribatid mites and three sequences of outgroups. To eliminate poorly aligned positions and divergent regions of the alignment, this final alignment (1490 bp) of 79 sequences was shortened based on the preliminary alignment using the Gblocks 0.91b with ‘allow gap positions within the final blocks’ option.

Supplementary files

Supplementary_materials_for_Methods_SuppInfo.docx ===== This file contains (a) DNA extraction, PCR and sequencing of oribatid mites from the two mountains and (b) Phylogeny of the all representative oribatid mites in Eurasia.

Figure S1-S4_SuppInfo.pdf ===== This file contains four supplementary figures. 

TableS1_15N_SuppInfo.xlsx ===== Oribatid mites from the Alps and Changbai Mountain with their mean and standard deviation values in 15N and number of measurements at each mountain. Species names in bold imply that they occurred at both of the two mountains.

TableS2_range_size_meta_data_SuppInfo.xlsx ===== The geographic regions of the occurrence of oribatid mites according to Subías (2022), their English translation, their area size (km²) and the internet source from which the respective size of each region was extracted.

TableS3_dataset_1-9_SuppInfo.xlsx ===== 18S sequence from the Alps, Changbai Mountain and NCBI for creating the Eurasia oribatid species phylogeny tree. Their family, superfamily, infraorder and geographical regions were given according to Subías (2022). Specie which accession number is in read means it was generated form this study. See Materials and Methods for more details about Dataset 1–9.

TableS4_species_age_and_mountain_stages_SuppInfo.xlsx ===== The node age of each species extracted from the ultrametric tree generated by BEAST (Figure S4) and different mountain uplift stages at the Alps and Changbai Mountain, respectively. Species names in bold imply that they occurred at both of the two mountains.

TableS5_range_size_species_SuppInfo.xlsx ===== Oribatid mites from the Alps and Changbai Mountain with their geographical regions and geographical range size (km²). Species names in bold imply that they occurred at both of the two mountains.

Code/Software

Rscript.R ===== All data were analysed in R (version 4.1.3), including the packages “stats”, “ape”, “vegan”, “phytools”, “caper”, and “ggplot2”. The R script is structured by different headers/ comments leading through the script. The R script can be used to reproduce results.