Skip to main content
Dryad

NE contribution to rebooting unconsciousness caused by midazolam

Data files

Nov 11, 2024 version files 112.59 KB

Abstract

The advent of midazolam holds profound implications for modern clinical practice. The hypnotic and sedative effects of midazolam afford it broad clinical applicability. However, the specific mechanisms underlying the modulation of altered consciousness by midazolam remain elusive.Therefore,we collated data on changes in time to consciousness recovery in mice through pharmacological, optogenetics, chemogenetics,fiber photometry, and gene knockout interventions to reveal the role of the locus cyanulus (LC) -ventrolateral preoptic nucleus (VLPO) noadrenergic neural circuit  in regulating midazolam-induced altered consciousness. This effect was mediated by α1 adrenergic receptors. Moreover, gamma-aminobutyric acid receptor type A (GABAA-R) represents a mechanistically crucial binding site in the LC for midazolam. These findings will provide novel insights into the neural circuit mechanisms underlying the recovery of consciousness after midazolam administration and will help guide the timing of clinical dosing and propose effective intervention targets for timely recovery from midazolam-induced loss of consciousne.