Data from: Another Fennoscandian Tortella species with fragile leaves, Tortella fragmenta (Pottiaceae, Bryophyta)
Data files
Apr 03, 2025 version files 191.41 KB
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README.md
3.15 KB
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Tortella_fragilis_atpB.txt
54.92 KB
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Tortella_fragilis_ITS.txt
80.57 KB
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Tortella_fragilis_rps4.txt
52.76 KB
Abstract
The well-known limestone species Tortella fragilis consists of two species, which are clearly circumscribed by the nuclear molecular marker ITS, and the plastid atpB-rbcL and rps4. The morphological differences between the two are relatively small. Because all specimens cannot be identified with certainty they could be understood as semi-cryptic. The two differ in the orientation of dry leaves and the appearance of papillae on lamina cells in mid-leaf. Dry leaves of T. fragilis s.str. are often screwed or slightly so, and near the shoot apex numerous leaves are sometimes tightly adhering (or parallel) to each other and together slightly screwed, or sometimes more loosely so or somewhat curled, and the lamina cells are densely papillose with papillae that are difficult to distinguish individually and obscure the cell walls. In T. fragmenta, which is described as new, the leaves are at most slightly screwed, curled, also near shoot apex, and not or hardly adhering to each other, and the lamina cells are moderately densely papillose with distinct and sometimes forked papillae that do not obscure the cell walls. Tortella fragilis is widespread in Fennoscandia, whereas T. fragmenta occurs primarily in the mountain range, with scattered occurrences in the lowlands of the boreal zone. Both species occur in calcareous or base-rich sites, with a tendency for T. fragilis to grow in wetter sites than T. fragmenta.
https://doi.org/10.5061/dryad.3tx95x6ps
Description of the data and file structure
The uploaded files are sequence alignments for ITS, atpB-rbcL, and rps4.
Twenty-six Tortella fragilis (Drumm.) Limpr. s.l. specimens were studied molecularly, including two specimens which sequences were downloaded from GenBank (see Appendix 1 of the paper). Based on Hedenäs (2015), Köckinger & Hedenäs (2017), Köckinger, et al. (2018), and Köckinger & Hedenäs (2023), 53 specimens belonging to 15 other Tortella species (Appendix 1 of the paper), two specimens of Chionoloma tenuirostre (Hook. & Taylor) M. Alonso, M.J. Cano & J.A. Jiménez, and two of Trichostomum crispulum Bruch were selected as outgroup.
The nuclear internal transcribed spacers 1 and 2 (ITS) and the plastid atpB-rbcL spacer (atpB-rbcL) and the rps4 gene + trnS-rps4 spacer (rps4) were studied. The new sequences were generated as described by Hedenäs (2015).
Nucleotide sequence fragments were edited and assembled for each DNA region using PhyDE® 0.9971 (http://www.phyde.de/index.html; accessed 7 March 2024). In the final data set, all three sequences were available for all specimens. However, the ITS sequence of D1588 was based only on the forward reading since the reverse one did not work. The assembled sequences were aligned manually in PhyDE®. Regions of partially incomplete data in the beginning and end of the sequences were identified and were excluded from subsequent analyses. Gaps were coded using the simple indel coding of Simmons & Ochoterena (2000) in SeqState (Müller, K. 2005). Gaps provided additional evidence and this information was included in the analyses. Sample numbers in the uploaded files can be found, with corresponding specimen data and GenBank accession numbers, in Appendix 1 of the paper.
Cited references: Hedenäs (2015: DOI 10.1007/s00606-014-1159-9); Köckinger & Hedenäs (2017: DOI 10.1080/03736687.2017.1307313); Köckinger & Hedenäs (2023: DOI 10.25227/linbg.24903); Köckinger et al. (2018: DOI 10.1639/0007-2745-121.4.560); Simmons & Ochoterena (2000: DOI 10.1093/sysbio/49.2.369); Müller (2005: DOI 10.2165/00822942-200504010-00008).
Geographic range: For the target species: Fennoscandia, plus two GenBank specimens from Greenland and Gorno-Altai in Russia, respectively (for other species than the focal ones: mainly Europe)
Files: Tortella fragilis_ITS (ITS); Tortella fragilis_atpB.txt (atpB-rbcL); Tortella fragilis_rps4 (rps4)
Usage notes: The sequence alignments are in FASTA format. The positions in the files correspond with ITS, atpB-rbcL, and rps4, as follows:
File: Tortella fragilis_ITS
ITS positions: 1-832
ITS indel coding positions: 834-938
Note: for D1588 only the forward reading of the sequence worked.
File: Tortella fragilis_atpB.txt
atpB-rbcL positions: 1-596
atpB-rbcL indel coding positions: 598-637
File: Tortella fragilis_rps4
rps4 positions: 1-605
rps4 indel coding positions: 607-611
For the molecular part of this study, 24 specimens of Tortella fragilis s.l. from Fennoscandia were sequenced and sequences for two additional specimens, from Greenland and Gorno-Altai in Russia, were downloaded from GenBank. Based on Hedenäs (2015), Köckinger and Hedenäs (2017), Köckinger, et al. (2018), and (Köckinger and Hedenäs 2023), 53 specimens belonging to 15 other Tortella species, two specimens of Chionoloma tenuirostre, and two of Trichostomum crispulum Bruch were selected as outgroup.
The nuclear internal transcribed spacers 1 and 2 (ITS) and the plastid atpB-rbcL spacer (atpB-rbcL) and the rps4 gene + trnS-rps4 spacer (rps4) were studied. DNA was extraction and new sequences were generated as described by Hedenäs (2015; https://doi.org/10.1007/s00606-014-1159-9).
Nucleotide sequence fragments were edited and assembled for each DNA region using PhyDE® 0.9971 (http://www.phyde.de/index.html; accessed 7 March 2024). In the final data set, all three sequences were available for all specimens. However, the ITS sequence of D1588 was based only on the forward reading since the reverse one did not work. The assembled sequences were aligned manually in PhyDE®. Regions of partially incomplete data in the beginning and end of the sequences were identified and were excluded from subsequent analyses. Gaps were coded using the simple indel coding of Simmons and Ochoterena (2000) in SeqState (Müller, K. 2005).