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Dopamine activity in the tail of the striatum, DeepLabCut and MoSeq during novel object exploration

Watabe-Uchida, Mitsuko1; Akiti, Korleki1; Tsutsui-Kimura, Iku1; Xie, Yudi1; Mathis, Alexander1; Markowitz, Jeffrey2; Anyoha, Rockwell2; Datta, Sandeep Robert2; Weygandt Mathis, Mackenzie1; Uchida, Naoshige1

Research facility: Harvard University
Published May 23, 2022; Updated Nov 29, 2023 on Dryad. https://doi.org/10.5061/dryad.41ns1rnh2

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Abstract

In this study, we characterized dynamics of novelty exploration using multi-point tracking (DeepLabCut) and behavioral segmentation (MoSeq). Mice were habituated in an arena, and then a object was placed at the corner of the arena. We compared 4 groups of mice: one with presentation of a novel object (stimulus novelty), one with a presentation of a familiar object (contextual novelty), one with presentation of a novel object after ablation of dopamine neuorns that project to the tail of the striatum (TS), and one with presentation of a novel object after sham surgery. With a separate group of mice, dopamine activity in TS was recorded during novelty exploration.