Data from: A facilitated diffusion mechanism establishes the Drosophila Dorsal gradient
Data files
Nov 06, 2017 version files 6.13 GB
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bcd-Toll10b-bcd3'UTR.zip
217.59 MB
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cact_+;cact-lacZ_+.zip
290.37 MB
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dl_+;dl-dGFP_+.zip
255.44 MB
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dl_+;dl-dGFP,H2A-RFP_+ for bleaching.zip
59.87 MB
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dl_+;dl-dVenus_+.zip
382.60 MB
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dl_+;dl-mVenus_+.zip
314.28 MB
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dl_+;Tlrv18_+.zip
242.40 MB
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dl_+.zip
782.25 MB
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dl-paGFP.zip
370.87 MB
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dl,dl-mGFP_+.zip
207.76 MB
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dl,dl-mGFP,H2A-RFP_+ for bleaching.zip
41.75 MB
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dl;dl-dGFP_+.zip
325.75 MB
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dl;dl-dGFP.zip
288.64 MB
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dl;dl-lacZ.zip
144.43 MB
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dl;dl-mVenus_+.zip
208.72 MB
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dl;dl-mVenus.zip
113.38 MB
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Egfrt1_+.zip
136.10 MB
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Egfrt1;dlRC_+.zip
39.20 MB
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Egfrt1;dlRC.zip
10.85 MB
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EGFRt1.zip
116.50 MB
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gd7;bcd-Toll10b-bcd3'UTR.zip
234.60 MB
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gd7;hsp83-Toll10b-bcd3'UTR.zip
212.71 MB
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README_for_bcd-Toll10b-bcd3'UTR.txt
1.08 KB
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README_for_cact_+;cact-lacZ_+.txt
1.08 KB
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README_for_dl_+;dl-dGFP_+.txt
1.08 KB
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README_for_dl_+;dl-dGFP,H2A-RFP_+ for bleaching.txt
1.08 KB
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README_for_dl_+;dl-dVenus_+.txt
1.08 KB
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README_for_dl_+;dl-mVenus_+.txt
1.08 KB
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README_for_dl_+;Tlrv18_+.txt
1.08 KB
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README_for_dl_+.txt
1.08 KB
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README_for_dl-paGFP.txt
1.08 KB
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README_for_dl,dl-mGFP_+.txt
1.08 KB
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README_for_dl,dl-mGFP,H2A-RFP_+ for bleaching.txt
1.08 KB
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README_for_dl;dl-dGFP_+.txt
1.08 KB
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README_for_dl;dl-dGFP.txt
1.08 KB
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README_for_dl;dl-lacZ.txt
1.08 KB
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README_for_dl;dl-mVenus_+.txt
1.08 KB
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README_for_dl;dl-mVenus.txt
1.08 KB
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README_for_Egfrt1_+.txt
1.08 KB
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README_for_Egfrt1;dlRC_+.txt
1.08 KB
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README_for_Egfrt1;dlRC.txt
1.08 KB
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README_for_EGFRt1.txt
1.08 KB
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README_for_gd7;bcd-Toll10b-bcd3'UTR.txt
1.08 KB
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README_for_gd7;hsp83-Toll10b-bcd3'UTR.txt
1.08 KB
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README_for_yw.txt
1.08 KB
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yw.zip
1.13 GB
Abstract
The transcription factor NF-κB plays an important role in the immune system, apoptosis and inflammation. Dorsal, a Drosophila homolog of NF-κB, patterns the dorsal-ventral axis in the blastoderm embryo. During this stage, Dorsal is sequestered outside the nucleus by the IκB homolog Cactus. Toll signaling on the ventral side breaks the Dorsal/Cactus complex, allowing Dorsal to enter the nucleus to regulate target genes. Fluorescent data show that Dorsal accumulates on the ventral side of the syncytial blastoderm. Here, we use modeling and experimental studies to show that this accumulation is caused by facilitated diffusion, or shuttling, of the Dorsal/Cactus complex. We also show that active Toll receptors are limiting in wild-type embryos, which is a key factor in explaining global Dorsal gradient formation. Our results suggest that shuttling is necessary for viability of embryos from mothers with compromised dorsal levels. Therefore, Cactus not only has the primary role of regulating Dorsal nuclear import, but also has a secondary role in shuttling. Given that this mechanism has been found in other, independent, systems, we suggest that it might be more prevalent than previously thought.