Data from: Construction and characterization of synthetic bacterial community for experimental ecology and evolution
Data files
Jul 24, 2019 version files 394.37 KB
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HMC33_antibiotic_MIC.txt
1.48 KB
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HMC33_Biolog_EcoPlate.txt
2.98 KB
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HMC33_genomic_features.txt
310.51 KB
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HMC33_MAPLE-2.3.0.txt
79.40 KB
Abstract
Experimental microbial ecology and evolution have yielded foundational insights into ecological and evolutionary processes using simple microcosm setups and phenotypic assays with one- or two-species model systems. The fields are now increasingly incorporating more complex systems and exploration of the molecular basis of observations. For this purpose, simplified, manageable and well-defined multispecies model systems are required that can be easily investigated using culturing and high-throughput sequencing approaches, bridging the gap between simpler and more complex synthetic or natural systems. Here we address this need by constructing a completely synthetic 33 bacterial strain community that can be cultured in simple laboratory conditions. We provide whole-genome data for all the strains as well as metadata about genomic features and phenotypic traits that allow resolving individual strains by amplicon sequencing and facilitate a variety of envisioned mechanistic studies. We further show that a large proportion of the strains exhibit coexistence in co-culture over serial transfer for 48 days in the absence of any experimental manipulation to maintain diversity. The constructed bacterial community can be a valuable resource in future experimental work.