Data from: Predation fear modulated thermal traits of the harmful algal bloom species Phaeocystis globosa
Data files
Jan 03, 2025 version files 101.95 KB
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Data__Fear_Phaecystis_Warming_Ye.xlsx
98.65 KB
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README.md
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Abstract
To evaluate whether non-consumptive effects alter thermal responses, P. globosa grown alone and those exposed to grazing cues associated with ciliates and copepods were maintained at temperatures ranging from 13°C to 30°C. P. globosa exposed to both grazing risks exhibited strong defensive responses, including higher colony abundances and greater colony diameters. P. globosa with induced defenses developed colonies at 15–30°C, whereas non-induced P. globosa formed colonies only within the narrow range of 17–25°C. Predation risks increased P. globosa tolerance to extreme temperatures, potentially explaining the occurrence of colonial blooms at lower latitudes. In addition, exposure to grazing by ciliates and copepods significantly expanded the breadth of the thermal performance curve by increasing the critical thermal maximum by 4.85°C, thereby reducing the vulnerability of P. globosa blooms to warming. We provide data on the influence of predation risk on the thermal trait of P. globosa.
https://doi.org/10.5061/dryad.573n5tbjb
Description of the data and file structure
To evaluate whether non-consumptive effects alter thermal responses, P. globosa grown alone and those exposed to grazing cues associated with ciliates and copepods were maintained at temperatures ranging from 13°C to 30°C. The incubations lasted for seven days. Solitary cell abundances, colony numbers, colony diameters, and cells per colony of P. globosa grown alone and exposed to grazing cues at different temperatures were determined. We also quantified TPCss using the equation by Thomas et al. 2012.
Files and variables
File: Data__Fear_Phaecystis_Warming_Ye.xlsx
Description: This dataset contains six sets of data on predation risk on thermal traits of Phaeocystis globosa. We provided data on solitary cell abundance, colony numbers, growth rate, colony size, and cell per colony of P. globosa grown alone and exposed to grazing risk associated with ciliates and copepods at 13, 15, 17, 21, 25, 28, and 30℃.
- Solitary cell abundance (cells per ml): This dataset contains 168 data points. 84 data points exhibited solitary cell abundance of P. globosa grown alone and exposed to grazing risk associated with ciliates and copepods (position: outside cage) at different temperatures. 84 data points exhibited solitary cell abundance of P. globosa grown alone and exposed to ciliates and copepods (position: inside cage) at different temperatures.
- Colony numbers (colonies per ml): We provided 168 data points to determine the interactive effects of temperature and grazing risk on colony formation in P. globosa. 84 data points exhibited colony numbers of P. globosa grown alone and exposed to grazing risk associated with ciliates and copepods (position: outside cage) at different temperatures. Another half of the dataset exhibited solitary cell abundance of P. globosa grown alone and exposed to ciliates and copepods (position: inside cage) at different temperatures.
- Rel_ size_ cell: In the present experiment, we measured 1393 data points of colony sizes and cells per colony . We compared the slopes of log (colony diameters) and log (cells per colony) between grazing treatment (P. globosa exposed to grazing risk) and controls (P. globosa grown alone) to assess whether grazing risk combined with temperature influenced colony architecture.
Growth rate: This dataset contains 95 data points showing the maximum growth rates of *P. globosa *when grown alone and exposed to grazing cues released from ciliates and copepods at different temperatures.
pH_25℃: We measured pH values in culture at 25℃ to assess whether pH influence growth and colony formation.
Nutrient_25℃: To assess whether nutrient concentrate affects growth and colony formation, nitrate, ammonium and phosphate concentration were determined.
Code/software
Plotting and statistical analyses were conducted with R (version 4.0.4, R development Core Team).
Access information
Other publicly accessible locations of the data:
- none
Data was derived from the following sources:
- none
Study organisms: P. globosa was cultured in f/2 medium in the exponential stage. Ciliates and copepods were fed the green alga Dunaliella tertiolecta every 3–4 days
Grazing risk experiment: P. globosa grew alone and exposed to grazing risks from the ciliate Euplotes vannus, and the copepod Apocyclops royi were maintained at temperatures of 13, 15, 17, 21, 25, 28, and 30°C.
Solitary cell concentrations: Solitary cell concentrations were measured using Sedawick-Rafter chambers. Cells were counted in randomly selected fields of view until 300 cells were counted per replicate.
Colony abundances: Colony abundances, colony diameters, and the number of cells per colony were measured in 24-well plates using an Olympus inverted microscope (Tang 2003; Wang et al. 2010). At least 30 colonies from each replicate were randomly chosen to measure colony diameters and cell numbers per colony.
Growth rates: 2 ml P. globosa samples from beakers were collected daily to measure in vivo chlorophyll fluorescence with a Turner Designs Trilogy fluorometer.
Nutrient concentrations: Nitrate, nitrite, phosphate and ammonium were determined following standard colourimetric methods using a Hitachi 722-S instrument (Grasshoff et al. 1999).
pH: pH values were measured using a Sentron pH meter (ArgusX, Cole-Parmer, USA).