Histone acetyltransferase p300/CBP regulates reproductive diapause via the juvenile hormone pathway in the cabbage beetle, Colaphellus bowringi

An, Hao-Min 1 2 ; Zhang, Yi-Ke1; Bai, Qian-Lu1; Liu, Wen3; Wang, Xiao-Ping1

Research facility: National Natural Science Foundation of China

Published Jul 14, 2025 on Dryad. https://doi.org/10.5061/dryad.79cnp5j7c

Data files

Jul 14, 2025 version files 663.61 MB

IMB_all_data.zip
663.57 MB
README.md
41.42 KB

Abstract

Juvenile hormone (JH) serves as a pivotal endocrine regulator of reproductive diapause in insects, though the molecular mechanisms governing JH signaling remain poorly characterized. This investigation assesses the roles of the histone acetyltransferase p300/CBP in mediating reproductive diapause through JH signaling in the cabbage beetle Colaphellus bowringi. RNA interference-mediated suppression of p300/CBP in reproductive female adults induced diapause-like phenotypes, including arrested ovarian maturation, suppressed vitellogenesis, enhanced lipid storage, and upregulated expression of diapause-associated genes. Along with downregulation of key JH biosynthetic enzymes and signaling components. While exogenous JH application decreased lipid accumulation, it failed to rescue ovarian developmental arrest. Parallel experiments in diapausing females with p300/CBP knockdown yielded similar results - JH analog treatment reduced lipid reserves without stimulating ovarian development. These findings collectively demonstrate that p300/CBP regulates photoperiodic reproductive diapause through dual regulation of JH biosynthesis and signal transduction pathways in C. bowringi. Our results establish p300/CBP as a critical mediator of JH biosynthesis and signaling during photoperiod-induced reproductive diapause. This work advances our understanding of epigenetic regulation in insect diapause and provides a molecular framework for future studies on environmental adaptation strategies in insects.

General information

This dataset does not include large-scale datasets such as transcriptomes; instead, it is organized according to the figures and tables in the article. It primarily comprises RT-qPCR data and various biochemical assay results. The calculated data from RT-qPCR, TG, TL, WB, ovarian size measurements, lipid droplet size measurements, and HPLC/MSMS are provided in CSV format, while other incompatible data are presented in PPT files. Additionally, anatomical images of the cabbage beetle ovaries are included.

Corresponding author information

Name: Xiao-Ping Wang
ORCID: https://orcid.org/0000-0002-1718-1026
Affiliation: Hubei Key Laboratory of Insect Resources Utilization and Sustainable Pest Management, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
email: xpwang@mail.hzau.edu.cn

Alternative contact information

Name: Hao-Min An
ORCID: https://orcid.org/0009-0002-3615-598X
Affiliation: Hubei Key Laboratory of Insect Resources Utilization and Sustainable Pest Management, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Institute for Advanced Study, Shenzhen University, Shenzhen 518060, China.
email: haominan@szu.edu.cn, ahm1994@126.com

Funding information

This work was supported by National Natural Science Foundation of China (NSFC) grants (32172391). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Dryad dataset

Dataset DOI: 10.5061/dryad.79cnp5j7c

Description of the data and file structure

We used dsRNA to knock down the key lysine acetyltransferase p300/CBP gene in the cabbage beetle (Colaphellus bowringi), a Coleopteran vegetable pest that undergoes reproductive diapause induced by a long photoperiod. By measuring ovarian phenotypes, lipid accumulation levels, juvenile hormone (JH) synthesis, and JH response levels, we analyzed the mechanism by which p300/CBP regulates the occurrence of reproductive diapause in the cabbage beetle through the JH pathway. Additionally, we combined methoprene induction experiments to analyze the specificity of p300/CBP in regulating the ovarian response to JH signaling in the cabbage beetle. SD means short-day, reproductive females. LD means long-day, diapause females. MG means mid-gut, FB means fat bodies, OVA means ovaries. Fig 1c is a schematic diagram of the RNAi target region and contains no actual data; therefore, it is not included in this dataset.

Files and variables

File: IMB_all_data.zip

File names

When applicable, file names follow the pattern

eut.(fig1).R(fig2).(fig3).(fig4).(fig5).(file_extension), where:

(fig1)
- fig1a_Temporal_expression_pattern.csv
  - group for different group include heads of LD-0dA-head,SD-0dA-head,LD-1dA-head,SD-1dA-head,LD-2dA-head,SD-2dA-head,LD-3dA-head,SD-3dA-head,LD-4dA-head SD-4dA-head
  - biological replicate for different biological replicates include 3 biological replicates such as biological replicate 1,biological replicate 2,biological replicate 3
  - technical replicate for different technical replicates include 2 technical replicates such as technical replicate 1,technical replicate 2
  - ct value of CBP for ct value of CBP
  - ct value of rpl19 for ct value of rpl19
  - ct value of act1 for ct value of act1
  - Geometric mean of references for mean ct values calculation
  - ΔCt (target-reference) for ΔCt values calculation
  - mean ΔCt in technical replicate for mean ΔCt in technical replicate calculation
  - mean ΔCt in control group for mean ΔCt in control group calculation
  - ΔΔCt(target gene-reference) for ΔΔCt(target gene-reference) calculation
  - 2^-ΔΔCt) for 2^-ΔΔCt calculation
- fig1b_Tissue_expression_pattern.CSV
  - group for different group include SD-2dA-MG,SD-2dA-head,SD-2dA-FB,SD-2dA-OVA
  - biological replicate for different biological replicates include 3 biological replicates such as biological replicate 1,biological replicate 2,biological replicate 3
  - technical replicate for different technical replicates include 2 technical replicates such as technical replicate 1,technical replicate 2
  - ct value of CBP for ct value of CBP
  - ct value of rpl19 for ct value of rpl19
  - ct value of act1 for ct value of act1
  - Geometric mean of references for mean ct values calculation
  - ΔCt (target-reference) for ΔCt values calculation
  - mean ΔCt in technical replicate for mean ΔCt in technical replicate calculation
  - mean ΔCt in control group for mean ΔCt in control group calculation
  - ΔΔCt(target gene-reference) for ΔΔCt(target gene-reference) calculation
  - 2^-ΔΔCt) for 2^-ΔΔCt calculation
- fig1d_SD+P-dsCBP_Mortality.csv
  - group for different group include SD+P-dsGFP1,SD+P-dsGFP2,SD+P-dsGFP3,SD+P-dsCBP1,SD+P-dsCBP2,SD+P-dsCBP3
  - death number for count death number
  - Mortality % for mortality calculation
- fig1e_SD_0dA_dsCBP_Survival_rate.CSV
  - n=30 means each group include 30 individuals
  - days after RNAi means days after dsRNA injection
  - SD+dsGFP means dsGFP injection control
  - SD+dsCBP means dsCBP injection
- fig1f_SD+dsCBP_RNAi_efficiency.CSV
  - group for different group include 4-day-old female adults whole bodies of SD+dsGFP,SD+dsCBP
  - biological replicate for different biological replicates include 3 biological replicates such as biological replicate 1,biological replicate 2,biological replicate 3
  - technical replicate for different technical replicates include 2 technical replicates such as technical replicate 1,technical replicate 2
  - ct value of CBP for ct value of CBP
  - ct value of rpl19 for ct value of rpl19
  - ct value of act1 for ct value of act1
  - Geometric mean of references for mean ct values calculation
  - ΔCt (target-reference) for ΔCt values calculation
  - mean ΔCt in technical replicate for mean ΔCt in technical replicate calculation
  - mean ΔCt in control group for mean ΔCt in control group calculation
  - ΔΔCt(target gene-reference) for ΔΔCt(target gene-reference) calculation
  - 2^-ΔΔCt) for 2^-ΔΔCt calculation
- fig1g_ovary_class.csv
  - group for different group include SD+0dA+dsGFP,SD+0dA+dsCBP-F1,SD+0dA+dsCBP-F2
  - class for different developmental class of ovaries include class 0,Class I,Class II,Class III,Class IV
  - Standard The criteria for classifying ovarian stages. Undifferentiated ovarian tubules lacking vitellogenin deposition=class 0,Ovarian tubule enlargement without vitellogenin deposition=Class I,Expanded ovarian tubules containing vitellogenin deposits but lacking defined eggs=Class II,Developed ovarian tubules with vitellogenin deposits and distinct immature eggs (spherical, size-variable precursors)=Class III,Fully matured ovarian tubules packed with uniform oval-shaped eggs=Class IV
  - numbers numbers of ovaries of class 0,Class I,Class II,Class III and Class IV
  - % Percentage of class 0,Class I,Class II,Class III oand Class IV
- fig1h_SD+dsCBP_ovary_phenotype.zip include ovary phenotype pics and ovary size sft format files of SD+0dA+dsGFP,SD+0dA+dsCBP-F1,SD+0dA+dsCBP-F2. Files in sft format can be opened with ScopePhoto. The ScopePhoto software can be downloaded from the address at the end of this document.
- fig1i_ovary size.csv
  - group for different group include SD+0dA+dsGFP,SD+0dA+dsCBP-F1,SD+0dA+dsCBP-F2
  - figure ovary figure name in fig1h_SD+dsCBP_ovary_phenotype.zip
  - Length/Pixels The pixel values of ovarian length measured using ScopePhoto
  - 1mm Scale/Pixels The pixel values of 1mm Scale
  - Actual Length The actual length obtained by dividing Length/Pixels by 1mm Scale/Pixels
  - Width/Pixels The pixel values of ovarian width measured using ScopePhoto
  - Actual Width The actual Width obtained by dividing Width/Pixels by 1mm Scale/Pixels
- fig1j_SD+dsCBP_vg1_vg2_RTqPCR.CSV
  - group for different group include 4-day-old female adults fat bodies of SD+dsGFP,SD+dsCBP
  - biological replicate for different biological replicates include 3 biological replicates such as biological replicate 1,biological replicate 2,biological replicate 3
  - technical replicate for different technical replicates include 2 technical replicates such as technical replicate 1,technical replicate 2
  - ct value of vg1 for ct value of vg1
  - ct value of vg2 for ct value of vg2
  - ct value of rpl19 for ct value of rpl19
  - ct value of act1 for ct value of act1
  - Geometric mean of references for mean ct values calculation
  - ΔCt (target-reference) for ΔCt values calculation
  - mean ΔCt in technical replicate for mean ΔCt in technical replicate calculation
  - mean ΔCt in control group for mean ΔCt in control group calculation
  - ΔΔCt(target gene-reference) for ΔΔCt(target gene-reference) calculation
  - 2^-ΔΔCt) for 2^-ΔΔCt calculation
- fig1k_SD+dsCBP_JHAMT1_HMGR2_FPPS1_RTqPCR.CSV
  - group for different group include 4-day-old female adults heads of SD+dsGFP,SD+dsCBP
  - biological replicate for different biological replicates include 3 biological replicates such as biological replicate 1,biological replicate 2,biological replicate 3
  - technical replicate for different technical replicates include 2 technical replicates such as technical replicate 1,technical replicate 2
  - ct value of JHAMT1 for ct value of JHAMT1
  - ct value of HMGR2 for ct value of HMGR2
  - ct value of FPPS1 for ct value of FPPS1
  - ct value of rpl19 for ct value of rpl19
  - ct value of act1 for ct value of act1
  - Geometric mean of references for mean ct values calculation
  - ΔCt (target-reference) for ΔCt values calculation
  - mean ΔCt in technical replicate for mean ΔCt in technical replicate calculation
  - mean ΔCt in control group for mean ΔCt in control group calculation
  - ΔΔCt(target gene-reference) for ΔΔCt(target gene-reference) calculation
  - 2^-ΔΔCt) for 2^-ΔΔCt calculation
- fig1l_SD+dsCBP_JHAMT1_WB.jpg include WB data of JHAMT1 in 2-day-old female adults heads of SD+dsGFP and SD+dsCBP
- fig1m_SD+dsCBP_JHAMT1_relative_protein_level.CSV
  - group for different group include -day-old female adults heads SD+dsGFP1,SD+dsGFP2,SD+dsGFP3,SD+dsCBP1,SD+dsCBP2,SD+dsCBP3
  - JHAMT1 values of JHAMT1 protein
  - Average value of JHAMT1 in GFP average value calculation of JHAMT1 protein value
  - Relative JHAMT1 caculation of relative JHAMT1 protein level
  - α Tublin values of α Tublin protein
  - JAverage value of α Tublin in GFP average value calculation of α Tublin protein value
  - Relative α Tublin caculation of relative α Tublin protein level
  - JHAMT1/α Tublin caculation of relative JHAMT1 protein level to α Tublin protein level
- fig1n_SD+dsCBP_HPLC_MSMS.CSV
  - Hemolymph volume hemolymph volume from 4-day-old female adults of dsGFP and dsCBP is 50μL
  - Concentrated volume volume of sample after extraction, concentration, and reconstitution
  - Concentration factor concentration factor (fold) of the sample during preparation of the final assay solution
  - JH III (ng/mL)=(Peak area+379686)/25553/Concentration factor JH titer Calculation Formula
  - group for different group include SD+dsGFP1,SD+dsGFP2,SD+dsGFP3,SD+dsCBP1,SD+dsCBP2,SD+dsCBP3
  - Peak area average value calculation of α Tublin protein value
  - JH III (ng/mL) caculation of relative α Tublin protein level
- fig1n_SD+dsCBP_HPLC_MSMS.jpg include HPLC/MSMS data of standard JHIII and samples of SD+dsGFP and SD+dsCBP
- fig1o_SD+dsCBP_krh1_JHE1_RTqPCR.CSV
  - group for different group include 4-day-old reproductive female adults fat bodies of SD+dsGFP,SD+dsCBP
  - biological replicate for different biological replicates include 3 biological replicates such as biological replicate 1,biological replicate 2,biological replicate 3
  - technical replicate for different technical replicates include 2 technical replicates such as technical replicate 1,technical replicate 2
  - ct value of krh1 for ct value of kr-h1
  - ct value of JHE1 for ct value of JHE1
  - ct value of rpl19 for ct value of rpl19
  - ct value of act1 for ct value of act1
  - Geometric mean of references for mean ct values calculation
  - ΔCt (target-reference) for ΔCt values calculation
  - mean ΔCt in technical replicate for mean ΔCt in technical replicate calculation
  - mean ΔCt in control group for mean ΔCt in control group calculation
  - ΔΔCt(target gene-reference) for ΔΔCt(target gene-reference) calculation
  - 2^-ΔΔCt) for 2^-ΔΔCt calculation
(fig2)
- fig2a_SD+dsCBP_Nile_red.jpg include nile red data of 4-day-old reproductive female adults fat bodies of SD+dsGFP and SD+dsCBP
- fig2b_SD+dsCBP_Nile_red_measurement.CSV
  - SD+dsGFP group of SD+dsGFP include 40 lipid droplets
  - SD+dsCBP group of SD+dsCBP include 40 lipid droplets
  - scale/μm photo scale is 100μm
  - pixel pixel value corresponding to the scale bar
  - length 1 measurement 1
  - length 2 measurement 2
  - average average of the two measurements
  - actual length/μm caculation of actual length
- fig2b_SD+dsCBP_Nile_red_measurement.jpg include nile red measurement pic
- fig2c_SD+dsCBP_TG.CSV
  - Group group of Ethanol(blank),1/3 standard,standard,4-day-old reproductive female adults whole bodies of SD-dsGFP-4d-1,SD-dsGFP-4d-2,SD-dsGFP-4d-3,SD-dsGFP-4d-4,SD-dsGFP-4d-5,SD-dsCBP-4d-1,SD-dsCBP-4d-2,SD-dsCBP-4d-3,SD-dsCBP-4d-4,SD-dsCBP-4d-5
  - OD510 OD510 value
  - average OD average OD510
  - sample OD-blank OD sample OD-blank OD
  - standard OD-blank OD standard OD-blank OD
  - (sample OD-blank OD)/(standard OD-blank OD) (sample OD-blank OD)/(standard OD-blank OD)
  - Standard concentration(mmol/L) Standard concentration(mmol/L)
  - [(sample OD-blank OD)/(standard OD-blank OD)]*Standard concentration [(sample OD-blank OD)/(standard OD-blank OD)]*Standard concentration
  - Fresh weight(gFw) Fresh weight value of each sample
  - sample TG concentration(gFW/L) sample TG concentration(gFW/L)
  - TG(mmol/gFw) TG value
  - average of SD-dsGFP average TG value
  - Relative TG=TG/(Average of SD-dsGFP) relative TG Calculation Formula
- fig2d_SD+dsCBP_Total_lipid.CSV
  - Group group of 4-day-old reproductive female adults whole bodies of SD+dsGFP-1 to SD+dsGFP-10,SD+dsCBP-1 to SD+dsCBP-10
  - Weight of the glass weighing bottle(W/g) Weight of the glass weighing bottle(W/g)
  - initial dry weight(IDW/g) initial dry weight(IDW/g)
  - dry weight after extration(EDW/g) dry weight after extration(EDW/g)
  - TL%=(IDW-EDW)/(IDW-W)×100 % TL Calculation Formula
- fig2e_SD+dsCBP_HSP21_23_70_RTqPCR.CSV
  - group for different group include 4-day-old reproductive female adults whole bodies of SD+dsGFP,SD+dsCBP
  - biological replicate for different biological replicates include 3 biological replicates such as biological replicate 1,biological replicate 2,biological replicate 3
  - technical replicate for different technical replicates include 2 technical replicates such as technical replicate 1,technical replicate 2
  - ct value of HSP21 for ct value of HSP21
  - ct value of HSP23 for ct value of HSP23
  - ct value of HSP70 for ct value of HSP70
  - ct value of rpl19 for ct value of rpl19
  - ct value of act1 for ct value of act1
  - Geometric mean of references for mean ct values calculation
  - ΔCt (target-reference) for ΔCt values calculation
  - mean ΔCt in technical replicate for mean ΔCt in technical replicate calculation
  - mean ΔCt in control group for mean ΔCt in control group calculation
  - ΔΔCt(target gene-reference) for ΔΔCt(target gene-reference) calculation
  - 2^-ΔΔCt) for 2^-ΔΔCt calculation
- fig2f_SD+dsCBP_DP1_RTqPCR.CSV
  - group for different group include 4-day-old reproductive female adults fat bodies of SD+dsGFP,SD+dsCBP
  - biological replicate for different biological replicates include 3 biological replicates such as biological replicate 1,biological replicate 2,biological replicate 3
  - technical replicate for different technical replicates include 2 technical replicates such as technical replicate 1,technical replicate 2
  - ct value of DP1 for ct value of DP1
  - ct value of rpl19 for ct value of rpl19
  - ct value of act1 for ct value of act1
  - Geometric mean of references for mean ct values calculation
  - ΔCt (target-reference) for ΔCt values calculation
  - mean ΔCt in technical replicate for mean ΔCt in technical replicate calculation
  - mean ΔCt in control group for mean ΔCt in control group calculation
  - ΔΔCt(target gene-reference) for ΔΔCt(target gene-reference) calculation
  - 2^-ΔΔCt) for 2^-ΔΔCt calculation
(fig3)
- fig3aSDi+ME_ovary_phenotype.zip include ovary phenotype pics and ovary size sft format files of 4-day-old reproductive female adults of SD+dsGFP+Ace,SD+dsCBP+Ace,SD+dsCBP+ME. Files in sft format can be opened with ScopePhoto. The ScopePhoto software can be downloaded from the address at the end of this document.
- fig3b_ovary_class.csv
  - group for different group include SD+dsGFP+Ace,SD+dsCBP+Ace,SD+dsCBP+ME
  - class for different developmental class of ovaries include class 0,Class I,Class II,Class III,Class IV
  - Standard The criteria for classifying ovarian stages. Undifferentiated ovarian tubules lacking vitellogenin deposition=class 0,Ovarian tubule enlargement without vitellogenin deposition=Class I,Expanded ovarian tubules containing vitellogenin deposits but lacking defined eggs=Class II,Developed ovarian tubules with vitellogenin deposits and distinct immature eggs (spherical, size-variable precursors)=Class III,Fully matured ovarian tubules packed with uniform oval-shaped eggs=Class IV
  - numbers numbers of ovaries of class 0,Class I,Class II,Class III and Class IV
  - % Percentage of class 0,Class I,Class II,Class III oand Class IV
- fig3c_ovary_size.csv
  - group for different group include SD+dsGFP+Ace,SD+dsCBP+Ace,SD+dsCBP+ME
  - figure ovary figure name in fig1h_SD+dsCBP_ovary_phenotype.zip
  - Length/Pixels The pixel values of ovarian length measured using ScopePhoto
  - 1mm Scale/Pixels The pixel values of 1mm Scale
  - Actual Length The actual length obtained by dividing Length/Pixels by 1mm Scale/Pixels
  - Width/Pixels The pixel values of ovarian width measured using ScopePhoto
  - Actual Width The actual Width obtained by dividing Width/Pixels by 1mm Scale/Pixels
- fig3d_SDi+ME_RNAi_efficiency.CSV
  - group for different group include 4-day-old reproductive female adults whole bodies of SD+dsGFP+Ace,SD+dsCBP+Ace,SD+dsCBP+ME
  - biological replicate for different biological replicates include 3 biological replicates such as biological replicate 1,biological replicate 2,biological replicate 3
  - technical replicate for different technical replicates include 2 technical replicates such as technical replicate 1,technical replicate 2
  - ct value of CBP for ct value of CBP
  - ct value of rpl19 for ct value of rpl19
  - ct value of act1 for ct value of act1
  - Geometric mean of references for mean ct values calculation
  - ΔCt (target-reference) for ΔCt values calculation
  - mean ΔCt in technical replicate for mean ΔCt in technical replicate calculation
  - mean ΔCt in control group for mean ΔCt in control group calculation
  - ΔΔCt(target gene-reference) for ΔΔCt(target gene-reference) calculation
  - 2^-ΔΔCt) for 2^-ΔΔCt calculation
- fig3e_SDi+ME_JHE1_krh1_RTqPCR.CSV
  - group for different group include 4-day-old reproductive female adults fat bodies of SD+dsGFP+Ace,SD+dsCBP+Ace,SD+dsCBP+ME
  - biological replicate for different biological replicates include 3 biological replicates such as biological replicate 1,biological replicate 2,biological replicate 3
  - technical replicate for different technical replicates include 2 technical replicates such as technical replicate 1,technical replicate 2
  - ct value of krh1 for ct value of kr-h1
  - ct value of JHE1 for ct value of JHE1
  - ct value of rpl19 for ct value of rpl19
  - ct value of act1 for ct value of act1
  - Geometric mean of references for mean ct values calculation
  - ΔCt (target-reference) for ΔCt values calculation
  - mean ΔCt in technical replicate for mean ΔCt in technical replicate calculation
  - mean ΔCt in control group for mean ΔCt in control group calculation
  - ΔΔCt(target gene-reference) for ΔΔCt(target gene-reference) calculation
  - 2^-ΔΔCt) for 2^-ΔΔCt calculation
- fig3f_SDi+ME_vg1_vg2_RTqPCR.CSV
  - group for different group include 4-day-old reproductive female adults fat bodies of SD+dsGFP+Ace,SD+dsCBP+Ace,SD+dsCBP+ME
  - biological replicate for different biological replicates include 3 biological replicates such as biological replicate 1,biological replicate 2,biological replicate 3
  - technical replicate for different technical replicates include 2 technical replicates such as technical replicate 1,technical replicate 2
  - ct value of vg1 for ct value of vg1
  - ct value of vg2 for ct value of vg2
  - ct value of rpl19 for ct value of rpl19
  - ct value of act1 for ct value of act1
  - Geometric mean of references for mean ct values calculation
  - ΔCt (target-reference) for ΔCt values calculation
  - mean ΔCt in technical replicate for mean ΔCt in technical replicate calculation
  - mean ΔCt in control group for mean ΔCt in control group calculation
  - ΔΔCt(target gene-reference) for ΔΔCt(target gene-reference) calculation
  - 2^-ΔΔCt) for 2^-ΔΔCt calculation
- fig3g_SDi+ME_Nile_red.jpg include nile red data of fat bodies of 4-day-old reproductive female adults of SD+dsGFP+Ace,SD+dsCBP+Ace,SD+dsCBP+ME
- fig3h_SDi+ME_Nile_red_measurement.CSV
  - SD+dsGFP+Ace group of 4-day-old reproductive female adults fat bodies of SD+dsGFP+Ace include 40 lipid droplets
  - SD+dsCBP+Ace group of 4-day-old reproductive female adults fat bodies of SD+dsCBP+Ace include 40 lipid droplets
  - SD+dsCBP+ME group of 4-day-old reproductive female adults fat bodies of SD+dsCBP+ME include 40 lipid droplets
  - scale/μm photo scale is 100μm
  - pixel pixel value corresponding to the scale bar
  - length 1 measurement 1
  - length 2 measurement 2
  - average average of the two measurements
  - actual length/μm caculation of actual length
- fig3h_SDi+ME_Nile_red_measurement.jpg include nile red measurement pic
- fig3i_SDi+ME_TG.CSV
  - Group group of Ethanol(blank),1/3 standard,standard,4-day-old reproductive female adults whole bodies of SD-dsGFP-Ace-1,SSD-dsGFP-Ace-2,SD-dsGFP-Ace-3,SD-dsCBP-Ace-1,SD-dsCBP-Ace-2,SD-dsCBP-Ace-3,SD-dsCBP-ME-1,SD-dsCBP-ME-2,SD-dsCBP-ME-3
  - average OD average OD510
  - sample OD-blank OD sample OD-blank OD
  - standard OD-blank OD standard OD-blank OD
  - (sample OD-blank OD)/(standard OD-blank OD) (sample OD-blank OD)/(standard OD-blank OD)
  - Standard concentration(mmol/L) Standard concentration(mmol/L)
  - [(sample OD-blank OD)/(standard OD-blank OD)]*Standard concentration [(sample OD-blank OD)/(standard OD-blank OD)]*Standard concentration
  - Fresh weight(gFw) Fresh weight value of each sample
  - sample TG concentration(gFW/L) sample TG concentration(gFW/L)
  - TG(mmol/gFw) TG value
  - average of SD-dsGFP-Ace average TG value
  - Relative TG=TG/(Average of SD-dsGFP-Ace) relative TG Calculation Formula
- fig3j_SDi+ME_total_lipid.CSV
  - Group group of 4-day-old reproductive female adults whole bodies of SD+dsGFP+Ace-1 to SD+dsGFP+Ace-13,SD+dsCBP+Ace-1 to SD+dsCBP+Ace-13,SD+dsCBP+ME-1 to SD+dsCBP+ME-13
  - Weight of the glass weighing bottle(W/g) Weight of the glass weighing bottle(W/g)
  - initial dry weight(IDW/g) initial dry weight(IDW/g)
  - dry weight after extration(EDW/g) dry weight after extration(EDW/g)
  - TL%=(IDW-EDW)/(IDW-W)×100 % TL Calculation Formula
(fig4)
- fig4a_LDi+ME_ovary_phenotype.zip include ovary phenotype pics and ovary size sft format files of 4-day-old diapause female adults of LD+dsGFP+Ace,LD+dsGFP+ME,LD+dsCBP+Ace,LD+dsCBP+ME. Files in sft format can be opened with ScopePhoto. The ScopePhoto software can be downloaded from the address at the end of this document.
- fig4b_ovary_class.csv
  - group for different group include LD+dsGFP+Ace,LD+dsGFP+ME,LD+dsCBP+Ace,LD+dsCBP+ME
  - class for different developmental class of ovaries include class 0,Class I,Class II,Class III,Class IV
  - Standard The criteria for classifying ovarian stages. Undifferentiated ovarian tubules lacking vitellogenin deposition=class 0,Ovarian tubule enlargement without vitellogenin deposition=Class I,Expanded ovarian tubules containing vitellogenin deposits but lacking defined eggs=Class II,Developed ovarian tubules with vitellogenin deposits and distinct immature eggs (spherical, size-variable precursors)=Class III,Fully matured ovarian tubules packed with uniform oval-shaped eggs=Class IV
  - numbers numbers of ovaries of class 0,Class I,Class II,Class III and Class IV
  - % Percentage of class 0,Class I,Class II,Class III oand Class IV
- fig4c_ovary_size.csv
  - group for different group include LD+dsGFP+Ace,LD+dsGFP+ME,LD+dsCBP+Ace,LD+dsCBP+ME
  - figure ovary figure name in fig1h_SD+dsCBP_ovary_phenotype.zip
  - Length/Pixels The pixel values of ovarian length measured using ScopePhoto
  - 1mm Scale/Pixels The pixel values of 1mm Scale
  - Actual Length The actual length obtained by dividing Length/Pixels by 1mm Scale/Pixels
  - Width/Pixels The pixel values of ovarian width measured using ScopePhoto
  - Actual Width The actual Width obtained by dividing Width/Pixels by 1mm Scale/Pixels
- fig4d_LDi+ME RNAi_efficiency_RTqPCR.CSV
  - group for different group include 4-day-old reproductive female adults whole bodies of LD+dsGFP+Ace,LD+dsGFP+ME,LD+dsCBP+Ace,LD+dsCBP+ME
  - biological replicate for different biological replicates include 3 biological replicates such as biological replicate 1,biological replicate 2,biological replicate 3
  - technical replicate for different technical replicates include 2 technical replicates such as technical replicate 1,technical replicate 2
  - ct value of CBP for ct value of CBP
  - ct value of rpl19 for ct value of rpl19
  - ct value of act1 for ct value of act1
  - Geometric mean of references for mean ct values calculation
  - ΔCt (target-reference) for ΔCt values calculation
  - mean ΔCt in technical replicate for mean ΔCt in technical replicate calculation
  - mean ΔCt in control group for mean ΔCt in control group calculation
  - ΔΔCt(target gene-reference) for ΔΔCt(target gene-reference) calculation
  - 2^-ΔΔCt) for 2^-ΔΔCt calculation
- fig4e_LDi+ME_Vg1_Vg2_RTqPCR.CSV
  - group for different group include 4-day-old reproductive female adults fat bodies of LD+dsGFP+Ace,LD+dsGFP+ME,LD+dsCBP+Ace,LD+dsCBP+ME
  - biological replicate for different biological replicates include 3 biological replicates such as biological replicate 1,biological replicate 2,biological replicate 3
  - technical replicate for different technical replicates include 2 technical replicates such as technical replicate 1,technical replicate 2
  - ct value of vg1 for ct value of vg1
  - ct value of vg2 for ct value of vg2
  - ct value of rpl19 for ct value of rpl19
  - ct value of act1 for ct value of act1
  - Geometric mean of references for mean ct values calculation
  - ΔCt (target-reference) for ΔCt values calculation
  - mean ΔCt in technical replicate for mean ΔCt in technical replicate calculation
  - mean ΔCt in control group for mean ΔCt in control group calculation
  - ΔΔCt(target gene-reference) for ΔΔCt(target gene-reference) calculation
  - 2^-ΔΔCt) for 2^-ΔΔCt calculation
- fig4f_LDi+ME_krh1_JHE1_RTqPCR.CSV
  - group for different group include 4-day-old reproductive female adults fat bodies of LD+dsGFP+Ace,LD+dsGFP+ME,LD+dsCBP+Ace,LD+dsCBP+ME
  - biological replicate for different biological replicates include 3 biological replicates such as biological replicate 1,biological replicate 2,biological replicate 3
  - technical replicate for different technical replicates include 2 technical replicates such as technical replicate 1,technical replicate 2
  - ct value of krh1 for ct value of kr-h1
  - ct value of JHE1 for ct value of JHE1
  - ct value of rpl19 for ct value of rpl19
  - ct value of act1 for ct value of act1
  - Geometric mean of references for mean ct values calculation
  - ΔCt (target-reference) for ΔCt values calculation
  - mean ΔCt in technical replicate for mean ΔCt in technical replicate calculation
  - mean ΔCt in control group for mean ΔCt in control group calculation
  - ΔΔCt(target gene-reference) for ΔΔCt(target gene-reference) calculation
  - 2^-ΔΔCt) for 2^-ΔΔCt calculation
- fig4g_LDi+ME_Nile_red.jpg include nile red data of fat bodies of 4-day-old reproductive female adults of LD+dsGFP+Ace,LD+dsGFP+ME,LD+dsCBP+Ace,LD+dsCBP+ME
- fig4h_LDi+ME_Nile_Red_measurement.CSV
  - LD+dsGFP+Ace group of LD+dsGFP+Ace include 40 lipid droplets
  - LD+dsGFP+ME group of LD+dsGFP+ME include 40 lipid droplets
  - LD+dsCBP+Ace group of LD+dsCBP+Ace include 40 lipid droplets
  - LD+dsCBP+ME group of LD+dsCBP+ME include 40 lipid droplets
  - scale/μm photo scale is 100μm
  - pixel pixel value corresponding to the scale bar
  - length 1 measurement 1
  - length 2 measurement 2
  - average average of the two measurements
  - actual length/μm caculation of actual length
- fig4h_LDi+ME_Nile_Red_measurement.jpg include nile red measurement pic
- fig4i_LDi+ME_TG.CSV
  - Group group of Ethanol(blank),1/3 standard,standard,4-day-old reproductive female adults whole bodies of LD-dsGFP-Ace-1,LD-dsGFP-Ace-2,LD-dsGFP-Ace-3,LD-dsGFP-Ace-4,LD-dsGFP-ME-1,LD-dsGFP-ME-2,LD-dsGFP-ME-3,LD-dsGFP-ME-4,LD-dsCBP-Ace-1,LD-dsCBP-Ace-2,LD-dsCBP-Ace-3,LD-dsCBP-Ace-4,LD-dsCBP-ME-1,LD-dsCBP-ME-2,LD-dsCBP-ME-3,LD-dsCBP-ME-4
  - average OD average OD510
  - average OD average OD510
  - sample OD-blank OD sample OD-blank OD
  - standard OD-blank OD standard OD-blank OD
  - (sample OD-blank OD)/(standard OD-blank OD) (sample OD-blank OD)/(standard OD-blank OD)
  - Standard concentration(mmol/L) Standard concentration(mmol/L)
  - [(sample OD-blank OD)/(standard OD-blank OD)]*Standard concentration [(sample OD-blank OD)/(standard OD-blank OD)]*Standard concentration
  - Fresh weight(gFw) Fresh weight value of each sample
  - sample TG concentration(gFW/L) sample TG concentration(gFW/L)
  - TG(mmol/gFw) TG value
  - average of LD+dsGFP+Ace average TG value
  - Relative TG=TG/(Average of LD+dsGFP+Ace) relative TG Calculation Formula
- fig4j_LDi+ME_total_lipid.CSV
  - Group group of 4-day-old reproductive female adults whole bodies of LD+dsGFP+Ace-1 to LD+dsGFP+Ace-13,LD+dsGFP+ME-1 to LD+dsGFP+ME-13,LD+dsCBP+Ace-1 to LD+dsCBP+Ace-13,LD+dsCBP+ME-1 to LD+dsCBP+ME-13
  - Weight of the glass weighing bottle(W/g) Weight of the glass weighing bottle(W/g)
  - initial dry weight(IDW/g) initial dry weight(IDW/g)
  - dry weight after extration(EDW/g) dry weight after extration(EDW/g)
  - TL%=(IDW-EDW)/(IDW-W)×100 % TL Calculation Formula
(fig5)
- fig5a_SDi+ME_TGL1_RTqPCR.CSV
  - group for different group include 4-day-old reproductive female adults fat bodies of SD+dsGFP+Ace,SD+dsCBP+Ace,SD+dsCBP+ME
  - biological replicate for different biological replicates include 3 biological replicates such as biological replicate 1,biological replicate 2,biological replicate 3
  - technical replicate for different technical replicates include 2 technical replicates such as technical replicate 1,technical replicate 2
  - ct value of TGL1 for ct value of TGL1
  - ct value of rpl19 for ct value of rpl19
  - ct value of act1 for ct value of act1
  - Geometric mean of references for mean ct values calculation
  - ΔCt (target-reference) for ΔCt values calculation
  - mean ΔCt in technical replicate for mean ΔCt in technical replicate calculation
  - mean ΔCt in control group for mean ΔCt in control group calculation
  - ΔΔCt(target gene-reference) for ΔΔCt(target gene-reference) calculation
  - 2^-ΔΔCt) for 2^-ΔΔCt calculation
- fig5b_LDi+ME_TGL1_RTqPCR.CSV
  - group for different group include 4-day-old diapause female adults fat bodies of LD+dsGFP+Ace,LD+dsGFP+ME,LD+dsCBP+Ace,LD+dsCBP+ME
  - biological replicate for different biological replicates include 3 biological replicates such as biological replicate 1,biological replicate 2,biological replicate 3
  - technical replicate for different technical replicates include 2 technical replicates such as technical replicate 1,technical replicate 2
  - ct value of TGL1 for ct value of TGL1
  - ct value of rpl19 for ct value of rpl19
  - ct value of act1 for ct value of act1
  - Geometric mean of references for mean ct values calculation
  - ΔCt (target-reference) for ΔCt values calculation
  - mean ΔCt in technical replicate for mean ΔCt in technical replicate calculation
  - mean ΔCt in control group for mean ΔCt in control group calculation
  - ΔΔCt(target gene-reference) for ΔΔCt(target gene-reference) calculation
  - 2^-ΔΔCt) for 2^-ΔΔCt calculation
- fig5c_SDi+ME_krh1_RTqPCR.CSV
  - group for different group include 4-day-old reproductive female adults fat bodies of SD+dsGFP+Ace,SD+dsCBP+Ace,SD+dsCBP+ME
  - biological replicate for different biological replicates include 3 biological replicates such as biological replicate 1,biological replicate 2,biological replicate 3
  - technical replicate for different technical replicates include 2 technical replicates such as technical replicate 1,technical replicate 2
  - ct value of krh1 for ct value of Kr-h1
  - ct value of rpl19 for ct value of rpl19
  - ct value of act1 for ct value of act1
  - Geometric mean of references for mean ct values calculation
  - ΔCt (target-reference) for ΔCt values calculation
  - mean ΔCt in technical replicate for mean ΔCt in technical replicate calculation
  - mean ΔCt in control group for mean ΔCt in control group calculation
  - ΔΔCt(target gene-reference) for ΔΔCt(target gene-reference) calculation
  - 2^-ΔΔCt) for 2^-ΔΔCt calculation
- fig5d_SDi+ME_Mcm4_RTqPCR.CSV
  - group for different group include 4-day-old reproductive female adults fat bodies of SD+dsGFP+Ace,SD+dsCBP+Ace,SD+dsCBP+ME
  - biological replicate for different biological replicates include 3 biological replicates such as biological replicate 1,biological replicate 2,biological replicate 3
  - technical replicate for different technical replicates include 2 technical replicates such as technical replicate 1,technical replicate 2
  - ct value of Mcm4 for ct value of Mcm4
  - ct value of rpl19 for ct value of rpl19
  - ct value of act1 for ct value of act1
  - Geometric mean of references for mean ct values calculation
  - ΔCt (target-reference) for ΔCt values calculation
  - mean ΔCt in technical replicate for mean ΔCt in technical replicate calculation
  - mean ΔCt in control group for mean ΔCt in control group calculation
  - ΔΔCt(target gene-reference) for ΔΔCt(target gene-reference) calculation
  - 2^-ΔΔCt) for 2^-ΔΔCt calculation
- fig5e_LDi+ME_krh1_RTqPCR.CSV
  - group for different group include 4-day-old diapause female adults fat bodies of LD+dsGFP+Ace,LD+dsGFP+ME,LD+dsCBP+Ace,LD+dsCBP+ME
  - biological replicate for different biological replicates include 3 biological replicates such as biological replicate 1,biological replicate 2,biological replicate 3
  - technical replicate for different technical replicates include 2 technical replicates such as technical replicate 1,technical replicate 2
  - ct value of krh1 for ct value of Kr-h1
  - ct value of rpl19 for ct value of rpl19
  - ct value of act1 for ct value of act1
  - Geometric mean of references for mean ct values calculation
  - ΔCt (target-reference) for ΔCt values calculation
  - mean ΔCt in technical replicate for mean ΔCt in technical replicate calculation
  - mean ΔCt in control group for mean ΔCt in control group calculation
  - ΔΔCt(target gene-reference) for ΔΔCt(target gene-reference) calculation
  - 2^-ΔΔCt) for 2^-ΔΔCt calculation
- fig5f_LDi+ME_Mcm4_RTqPCR.CSV
  - group for different group include 4-day-old diapause female adults fat bodies of LD+dsGFP+Ace,LD+dsGFP+ME,LD+dsCBP+Ace,LD+dsCBP+ME
  - biological replicate for different biological replicates include 3 biological replicates such as biological replicate 1,biological replicate 2,biological replicate 3
  - technical replicate for different technical replicates include 2 technical replicates such as technical replicate 1,technical replicate 2
  - ct value of Mcm4 for ct value of Mcm4
  - ct value of rpl19 for ct value of rpl19
  - ct value of act1 for ct value of act1
  - Geometric mean of references for mean ct values calculation
  - ΔCt (target-reference) for ΔCt values calculation
  - mean ΔCt in technical replicate for mean ΔCt in technical replicate calculation
  - mean ΔCt in control group for mean ΔCt in control group calculation
  - ΔΔCt(target gene-reference) for ΔΔCt(target gene-reference) calculation
  - 2^-ΔΔCt) for 2^-ΔΔCt calculation
- fig5g_SDi+ME_VgR_RTqPCR.CSV
  - group for different group include 4-day-old reproductive female adults fat bodies of SD+dsGFP+Ace,SD+dsCBP+Ace,SD+dsCBP+ME
  - biological replicate for different biological replicates include 3 biological replicates such as biological replicate 1,biological replicate 2,biological replicate 3
  - technical replicate for different technical replicates include 2 technical replicates such as technical replicate 1,technical replicate 2
  - ct value of VgR for ct value of VgR
  - ct value of rpl19 for ct value of rpl19
  - ct value of act1 for ct value of act1
  - Geometric mean of references for mean ct values calculation
  - ΔCt (target-reference) for ΔCt values calculation
  - mean ΔCt in technical replicate for mean ΔCt in technical replicate calculation
  - mean ΔCt in control group for mean ΔCt in control group calculation
  - ΔΔCt(target gene-reference) for ΔΔCt(target gene-reference) calculation
  - 2^-ΔΔCt) for 2^-ΔΔCt calculation
- fig5h_LDi+ME_VgR_RTqPCR.CSV
  - group for different group include 4-day-old diapause female adults fat bodies of LD+dsGFP+Ace,LD+dsGFP+ME,LD+dsCBP+Ace,LD+dsCBP+ME
  - biological replicate for different biological replicates include 3 biological replicates such as biological replicate 1,biological replicate 2,biological replicate 3
  - technical replicate for different technical replicates include 2 technical replicates such as technical replicate 1,technical replicate 2
  - ct value of VgR for ct value of VgR
  - ct value of rpl19 for ct value of rpl19
  - ct value of act1 for ct value of act1
  - Geometric mean of references for mean ct values calculation
  - ΔCt (target-reference) for ΔCt values calculation
  - mean ΔCt in technical replicate for mean ΔCt in technical replicate calculation
  - mean ΔCt in control group for mean ΔCt in control group calculation
  - ΔΔCt(target gene-reference) for ΔΔCt(target gene-reference) calculation
  - 2^-ΔΔCt) for 2^-ΔΔCt calculation

Description:

The data has been organized in the order of the figures.

Code/software

Scopephoto was used to measure the size of the ovaries or lipid droplets.

https://scopephoto.software.informer.com/3.1/