Dataset DOI: 10.5061/dryad.8w9ghx40n

Description of the data and file structure

This dataset collection presents the raw experimental data underlying laboratory bioassays that evaluated the mosquitocidal potential of Pseudomonas mosselii against dengue vector mosquitoes (Aedes aegypti and Aedes albopictus), as well as its effects on two non-target aquatic organisms (Culex quinquefasciatus and ostracods). The study assessed three formulations of the bacterial treatment, namely, cell-free supernatant (CFS), freeze-dried cell-free supernatant (FDCFS), and freeze-dried bacterial culture (FDBC), with treatment concentrations expressed in mg/mL. Each test concentration was replicated four times, along with four distilled water controls. Each dataset is provided in .csv format.

• Dataset 1 reports larval mortality of Aedes aegypti exposed to different concentrations of CFS, FDCFS, and FDBC over 24, 48, and 72 hours. Each treatment group involved 15 larvae per replicate.

• Dataset 2 presents the number of Aedes aegypti larvae reaching pupation and adult emergence after treatment exposure, with initial sample sizes of 15 larvae per concentration.

• Dataset 3 includes time-to-event data (days to pupation and adult emergence) for Aedes aegypti larvae hatched from eggs and continuously exposed to CFS, FDCFS, or FDBC. Twenty individuals were exposed per treatment condition.

• Dataset 4 mirrors Dataset 3 but focuses on Aedes albopictus larvae. It records the number of pupae and adults emerged, along with the development time. Each treatment condition had 20 exposed individuals.

• Dataset 5 contains mortality data for Culex quinquefasciatus larvae exposed to Pseudomonas mosselii treatments (CFS, FDCFS, FDBC) at various concentrations, with 15 larvae per treatment group.

• Dataset 6 records the mortality of non-target ostracods after exposure to FDCFS and FDBC, with 20 organisms per treatment group.

• Dataset 7 evaluates the long-term stability of the freeze-dried bacterial products (FDCFS and FDBC) by measuring larval mortality of Aedes aegypti at different storage intervals (immediate, 1 month, 2 months, and 3 months post-lyophilization). Each treatment replicate involved 15 larvae.

All experiments were conducted under laboratory conditions with ethical approval obtained from the  Ethical Review Committee of Postgraduate Institute of Science, University of Peradeniya, Sri Lanka (Certificate ID: CEC_PGIS_2024_12).

Files and variables

File: Dataset_1_RawData_Larval_Mortality-Aedes.csv

Description: This dataset records the number of dead Aedes aegypti larvae at different treatment concentrations of the cell-free supernatant (CFS), freeze-dried cell-free supernatant (FDCFS), and freeze-dried bacterial culture (FDBC) of Pseudomonas mosselii. Mortality data were collected at 24, 48, and 72 hours post-exposure. Each concentration was tested on 15 larvae per replicate, with four replicates per concentration and four parallel control replicates using distilled water. The treatment concentrations are expressed in milligrams per milliliter (mg/mL). This dataset represents acute toxicity data used to determine dose-response effects over time.

Variables

• Direct supernatant -CFS:
• Freeze dried supernatant - FDCFS:
• Freeze dried culture- FDBC:

File: Dataset_2_RawData_Aedes_Devlopment.csv

Description: This dataset presents the number of Aedes aegypti larvae that successfully reached the pupal and adult stages after exposure to Pseudomonas mosselii treatments of larval bioassays. The three formulations tested were cell-free supernatant (CFS), freeze-dried cell-free supernatant (FDCFS), and freeze-dried bacterial culture (FDBC). Each concentration was tested with 15 larvae per replicate, using four replicates per treatment and four distilled water controls. The data capture potential sublethal effects by measuring how each treatment impacts development into later life stages. NA means-No observations recorded

Variables

• Direct supernatant- CFS:
• Freeze dried Supernatant -FDCFS:
• Freeze dried bacterial culture- FDBC:

File: Dataset_4_RawData-Ovicidal_and_Developmental-Albopictus.csv

Description: Similar to Dataset 3, this dataset evaluates ovicidal and post-hatching developmental effects, but in Aedes albopictus. Eggs were exposed to Pseudomonas mosselii treatments, CFS, FDCFS, and FDBC and data were collected on the number of individuals reaching pupation and adult emergence, along with the number of days required to reach these stages. Each treatment was tested using 20 exposed eggs per replicate, with four replicates per concentration and four distilled water controls. The dataset allows for species-level comparison of developmental sensitivity to microbial treatment. NA means-No observations recorded

Variables

• Direct supernatant- CFS:
• Freeze dried Supernatant -FDCFS:
• Freeze dried bacterial culture- FDBC:

File: Dataset_3_RawData-Ovicidal_and_Development-_Aegypti.csv

Description: This dataset contains the results from ovicidal bioassays performed on Aedes aegypti. Eggs were exposed to the three treatment formulations of Pseudomonas mosselii, cell-free supernatant (CFS), freeze-dried cell-free supernatant (FDCFS), and freeze-dried bacterial culture (FDBC) and development of hatched larvae was monitored until adult emergence. The dataset includes the number of larvae that reached pupation and adulthood, as well as the time taken to reach these developmental stages, recorded in days. Twenty mosquito eggs were exposed per treatment, with four replicates per treatment and four controls. These data help assess whether exposure at the egg stage delays or suppresses mosquito development. NA means-No observations recorded

Variables

• Direct supernatant- CFS:
• Freeze dried Supernatant -FDCFS:
• Freeze dried bacterial culture- FDBC:

File: Dataset_5_RawData-Culex_larval_mortality.csv

Description: This dataset includes larval mortality data for Culex quinquefasciatus, a non-target mosquito species, exposed to the cell-free supernatant (CFS), freeze-dried cell-free supernatant (FDCFS), and freeze-dried bacterial culture (FDBC) of Pseudomonas mosselii. Mortality was recorded at 24, 48, and 72 hours post-exposure. Each concentration was tested using 15 larvae per replicate, with four replicates per treatment and four controls. The dataset is essential for evaluating the non-target specificity and environmental safety of the tested biolarvicides.

Variables

• Direct supernatant -CFS:
• Freeze dried supernatant - FDCFS:
• Freeze dried culture- FDBC:

File: Dataset_6_RawData_Non_target.csv

Description: This dataset documents mortality in ostracods (small aquatic crustaceans) exposed to the freeze-dried products of Pseudomonas mosselii, specifically, FDCFS and FDBC. Each treatment was tested with 15 ostracods per replicate, across four replicates per concentration and four controls. Mortality was recorded at 24 and 48 hours after exposure. These data contribute to the environmental risk assessment of microbial larvicides on aquatic microfauna.

Variables

• Freeze dried cell free supernatant-FDCFS:
• Freeze dried culture-FDBC:

File: Dataset_7_RawData_Shelf_life.csv

Description: This dataset evaluates the long-lasting larvicidal effects of the freeze-dried cell-free supernatant (FDCFS) and freeze-dried bacterial culture (FDBC) of Pseudomonas mosselii. Aedes aegypti larvae (15 per replicate) were exposed to the LC₅₀ concentration previously determined in Dataset 1. The treatments were stored at 4 °C and tested at four time intervals: immediately after lyophilization (0 months), and after 1, 2, and 3 months of storage. Mortality was assessed at 24, 48, and 72 hours post-exposure. Each time point included four replicates per treatment and four control replicates. This dataset reflects the storage stability and residual bioactivity of microbial larvicidal products. Merged cells in spreadsheets group treatment identifiers and replicate sets

Variables

• Freeze dried cell free supernatant -FDCFS:
• Freeze dried cell free supernatant -FDCFS:

Access information

Other publicly accessible locations of the data:

• No

Data was derived from the following sources:

• No