Female flowering traits influencing hybrid grain production in wheat
Data files
Nov 20, 2024 version files 51.52 KB
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FieldExp.csv
32.20 KB
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GreenhouseExp.csv
16.92 KB
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README.md
2.39 KB
Abstract
To better understand how to produce hybrid wheat grains more efficiently, we examined the impact of synchronized flowering between female (male-sterile) lines and their male cross-pollinator lines, as well as the effect of flowering duration on hybrid grain production. Over a three-year field trial, we found that synchronization of flowering times with the longest possible overlap had the most significant positive effect on hybrid grain production. However, even with adequate spatial and temporal synchronization, some female lines still produced fewer hybrid grains than others, indicating genetic differences in female flower receptivity. To assess female receptivity more accurately, we developed a new phenotyping scale for male-sterile wheat flowers, which provides the floral foundation for effective cross-pollination. Our field and greenhouse trials showed that the better-performing female lines remained in the pollen-receptive phase for a longer period.
https://doi.org/10.5061/dryad.dv41ns27q
Description of the data and file structure
FieldExp:
Data are presented for flowering, and other hybrid production-related traits on 4 winter wheat female lines, grown during 3 growth seasons on the field.
GreenhouseExp:
Data are presented for ovary length and Waddington Stage on 2 winter wheat female lines, grown in controlled conditions (greenhouse).
Files and variables
File: FieldExp.csv
Description:
Variables
- Plot: unique number for this plot
- Material: Genotype
- HD: Heading date in days after January 1st
- FLS: Flowering start in days after January 1st
- FLP: Flowering peak in days after January 1st (in 2020 this column represents the trait “SO” = “Second Opening”)
- FLE: Flowering end in days after January 1st
- PH: Plant height in cm
- Year: Year of trial
- yield: yield in gram per m²
- Rep: Replication
- SO_Beg: Second opening begin (only 2020 data, 2018 and 2019 n/a) in days after January 1st
- SO_End: Second opening end (only 2020 data, 2018 and 2019 n/a) in days after January 1st
- FLD: Flowering duration in days
- SOD: Second opening duration (only 2020 data, 2018 and 2019 n/a) in days
- male: Male genotype
- male_FLS: Male flowering start in days after January 1st
- male_FLP: Male flowering peak in days after January 1st
- male_FLE: Male flowering end in days after January 1st
- male_PH: Male plant height in cm
- CROSS: Crossed parents
- FLP_syn: Amount of days flowering of parents synchronized
- Nick_FLS: Nick-index calculated for Flowering start
- Nick_FLP: Nick-index calculated for flowering peak
- Nick_FLE: Nick-index calculated for flowering end
- Nick: Mean of Nick_FLS and Nick_FLP
- Nick_SO_Beg: Nick-index calculated for Second opening begin (only 2020 data, 2018 and 2019 n/a)
- Nick_SO: Nick-index calculated for Second opening (only 2020 data, 2018 and 2019 n/a)
n/a = Trait was not phenotyped
null = missing value
File: GreenhouseExp.csv
Description:
Variables
- Genotype: Genotypes
- Replication: Different plant used
- DAHD: Days after heading date
- Position: Spikelet position at the spike (count from bottom)
- Floret1: First floret ovary size in µm
- Floret2: Second floret ovary size in µm
- Stage: Waddington Stage+
FieldExp:
Whole plots were phenotyped in the field trial. The yield was recorded by harvesting with a plot combine harvester. Non-sterile plots were not included in the calculation. The Nick-index values were calculated afterward.
GreenhouseExp:
The length of floret 1 and 2 was determined using a Stemi 2000 microscope. The stages were visually phenotyped.