https://doi.org/10.5061/dryad.f1vhhmh60

Description of the data and file structure

The archived files include two .ZIP

CODE_ARCHIVE_FINAL.zip: R scripts and data for generating Figures 1, 2, 3, 5, and 6 of the main text, along with archived original Figure images. The archive is organized into sub-directories. There is a separate sub-directory for each figure. Each sub-directory has a .R script for generating the applicable figures.

• ./fig1
  • cv_temp.R simulates synthetic data to construct Figure 1 of the manuscript
• ./fig2
  • cv_temp_data.R digests the data (as described in the next bullet points) to generate Figure 2
  • ./Altman contains Altman_tadpoles_data.csv which are raw data from Altman et al. 2016 JAE, which are used to generate the lower panel of Figure 2. Note that these data were not collected by us as authors but are publicly available data that we used to create panels in our figure. The required fields from this data file are AdjPropEncysted and PerfTemp. However, all variables are described below. Also note that blank values (i.e., NA values) are sometimes present if the animal of question did not live to the time of that data measurement.
    • TadID Individual tadpole identification
    • Block Temporal block
    • Clutch Tadpole clutch of origin identification
    • AccGraph1 Category of tadpole acclimation temperature used for Fig. 3B of Altman et al.
    • AccGraph2 Category of tadpole acclimation temperature used for Fig. 4 (both A & B) of Altman et al.
    • AccTemp Tadpole acclimation temperature in degrees Celcius
    • AccInc Identification of the incubator in which the tadpole was kept during the 3-week acclimation period
    • PerfTemp Performance temperature in degrees Celcius
    • PerfInc Identification of the incubator in which the tadpole was kept during the performance period (following the temperature switch)
    • InitialGosner Tadpole Gosner stage at the beginning of the acclimation period
    • ExposureGosner Tadpole Gosner stage at time of exposure to R. ondatrae
    • FinalGosner Tadpole Gosner stage at the end of of the experiment (2 weeks post-exposure to R. ondatrae)
    • InitialMass Tadpole mass (mg) at the beginning of the acclimation period
    • ExposureMass Tadpole mass (mg) at time of exposure to R. ondatrae
    • FinalMass Tadpole mass (mg) at the end of the experiment (2 weeks post-exposure to R. ondatrae)
    • Cysts1 Number of metacercariae present in tadpole 12 hours post-exposure to R. ondatrae
    • Cysts7 Number of metacercariae present in tadpole 7 days post-exposure to R. ondatrae
    • Cysts14 Number of metacercariae present in tadpole 14 days post-exposure to R. ondatrae
    • PropCleared1 Proportion of metacercariae cleared by the tadpole between 12 hours and 7 days post-exposure to R. ondatrae (Fig. 4A of Altman et al.)
    • PropCleared2 Proportion of metacercariae cleared by the tadpole between 7 and 14 days post-exposure to R. ondatrae (Fig. 4B of Altman et al.)
    • PropEncysted Raw proportion of parasites to encyst in each tadpole (“Cysts1”/20)
    • LeftoverCerc Number of cercariae that failed to encyst in the tadpole (found in the exposure water after the 12 hour exposure period)
    • AdjPropEncysted Same as “PropEncysted”, except max value is 1 (1.05 values were changed to 1) to allow for arcsine square root transformation (Fig. 3B)
  • ./Six-Viruses-Temp contains raw data from Shocket et al. 2020 Elife, which are used to generate the top and middle panel of Figure 2. Shocket et al. 2020 gathered data from several studies. Note that these data were not collected by us as authors but are publicly available data that we used to create panels in our figure. We used two data files TraitData_bc.csv and TraitData_lf.csv. Note that we only considered Cx. pipiens (Cpip) and West Nile virus (WNV). The file attributes are defined as follows:
    • seriesID series ID with the name of the person who digitized the data from the published paper
    • trait.name trait abbreviation, which is often the model parameter
      • 1/mu, lifespan (days; the inverse of mortality rate, mu (lf 1/mu))
      • bc, vector competence (propotion; bc full vector competence # transmitting / # exposed; c infection efficiency # infected / # exposed; b transmission efficiency # transmitting / # infected)
    • T temperature (C)
    • trait trait value
    • ErrorPosSI & ErrorNegSI standard errors for the trait value, if “NA” then not enough data was available to calculate the value.
    • Trait2.name second variable in the experiment, if needed; for vector competence this is usually time
    • host.code four letter code for mosquito vector species
      • Cpip Cx. pipiens, Cqui Cx. quinquefasciatus, Ctar Cx. tarsalis, Cuni Cx. univittatus, Cthe Cx. theileri, Atri Ae. triseriatus, Atae Ae. taeniorhynchus, Avex Ae. vexans, Cmel Cs. melanura, Cmol Cx. pipiens molestus, Cpal Cx. pipiens pallens, Cres Cx. restuans, Ador Ae. dorsalis, Anig Ae. nigromaculis, Asol Ae. sollicitans, Asal Ae. salinarius
    • paras.code four letter code for parasite (virus)
    • Citation first author, year of publication, and journal name for publication the data came from
    • Figure the figure or table that the data came from in the original publication
    • Notes any notes
    • joint.code eight letter code for combination of mosqutio vector species and virus (host.code + paras.code, for infection traits only)
• ./fig3
  • fig3-R02.R simulates synthetic data to construct Figure 3 of the manuscript
• ./figs5_6
  • emp_var_SEIP.R simulates synthetic data to construct both Figures 5 and 6 of the manuscript

Code/software

ESM_FINAL.zip: R and QMD scripts for generating the Electronic Supplementary Materials files

• There are three file types: .R, .QMD, and .PDF
• The user can run the .QMD file, which sources the two .R scripts but should run as long as the user has specified the correct working directory.
• The .QMD can be rendered into .HTML or .PDF
• The two .R scripts contain helper functions
• The .PDF contains the archived version of the rendered .QMD