Data from: Development of a 3D simulator for training the mouse in utero electroporation
Data files
Mar 29, 2025 version files 5.67 KB
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Data_IUE_Model_Embryos.csv
788 B
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Data_IUE_Model_Survey.csv
405 B
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Data_IUE_Model_Uterus.csv
1.32 KB
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README.md
3.16 KB
Abstract
In utero electroporation (IUE) requires high-level training in microinjection through the mouse uterine wall into the lateral ventricle of the mouse brain. Training for IUE is currently being performed in live mice, as no artificial models allow simulations yet. This study aimed to develop an anatomically realistic 3D-printed simulator to train IUE in mice. To this end, we created embryo models containing lateral ventricles. We coupled them to uterus models in six steps: (1) computed tomography imaging, (2) 3D model segmentation, (3) 3D model refinement, (4) mold creation to cast the actual model, (5) 3D mold printing, and (6) mold casting the molds with a mix of soft silicones to ensure the hardness and consistency of the uterus and embryo. The results showed that the simulator assembly successfully recreated the IUE. The compression test did not differ in the mechanical properties of the real embryo or in the required load for uterus displacement. Furthermore, more than 90% of the users approved the simulator as an introduction to IUE and considered that the simulator could help reduce the number of animals for training. Despite current limitations, our 3D simulator enabled a realistic experience for initial approximations to the IUE and is a real alternative for implementing the 3Rs. We are currently working on refining the model.
https://doi.org/10.5061/dryad.n8pk0p2zp
Description of the data and file structure
A three-part mold for the uterus model was created, consisting of two outer parts for the exterior shape and an inner part representing the space for the embryos. The inner part had a tab to hold the silicone outer mold with a 0.5 mm gap. Cylinders and a cube from the Netfabb library were combined using the “Boolean union” function. The inner model was hollowed with a 0.5 mm offset, then combined with the cube. The outer model was subtracted from the outer box and separated using the “plane cut” function.
The details of the experimental design can be found in the corresponding publication.
The data incluce:
- Mechanical properties from embryos and their models
- Mechanical properties from uteri and their models
- Data of the survey evaluating of the IUE simulator
Files and variables
File: Data_IUE_Model_Survey.csv
The IUE simulation model was evaluated through a practical session with a questionnaire at the University Medical Center’s IUE facility. Users, classified as beginner/intermediate (n = 3) and expert (n = 3), practiced IUE surgery steps on the models and provided feedback on using this model as an alternative to live-animal training.
Description: User evaluation (%) to each of our questions to evaluate the IUE simulator
Questions
- Model rating overall
- Model rating for introduction
- Model rating practicing steps
- Model rating for replacement
- Model rating for reduction
File: Data_IUE_Model_Uterus.csv
Mice were sacrificed at E15, and their uteri were incised at both ends. Embryos were extracted carefully to avoid uterine damage. Surgical-grade thread was used to suture loops around the uterus, maintaining a 5 mm distance. The uterus was positioned on hooks of a force test device, which was tared to zero and set to move at 10 mm/min with a minimal force of 0.002 N. The device moved to a maximum of 30 mm while measuring the force increase.
Description: Load [N] per displacement [mm] as a measure of hardness
Variables
- Uterus (E15)
- Uterus silicone model (SH00-20)
- Uterus silicone model (SH00-30)
File: Data_IUE_Model_Embryos.csv
Shore00-10, 20, and 30 silicones were mixed, colored, and degassed before being poured into the mold. The upper head part was filled first, followed by the main body. The molds were closed with clamps, and silicones were allowed to harden overnight. Only Sh00-30 models were intact after demolding; others were destroyed. Silicone was prepared without coloring, poured into the mold, and the inner mold part inserted. The mold was clamped and left to harden overnight. The next day, the model was removed gently. Sh00-20 and Sh00-30 silicones were used for their similar elasticity to the embryo, with Sh00-30 selected for its easier demolding and handling properties.
Description: Load [N] per displacement [mm] as a measure of hardness
Variables
- Embryo
- Embryo silicone model