Soil microbe-induced plant volatiles can alert neighboring plants for tolerating heavy metal stress
Data files
Nov 02, 2024 version files 5.09 MB
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MIPV-data.xlsx
5.08 MB
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README.md
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Abstract
Induced volatiles can elicit defense or tolerance responses in neighboring receiver plants, a phenomenon referred to as the ‘alerting effect’. While the alerting effect of microbe-induced plant volatiles (MIPVs) to biotic stressors has been extensively studied, the function of MIPVs in relation to abiotic stressors has received less attention. Furthermore, whether changes in MIPVs can prime neighboring plants in preparation for subsequent heavy metal stress remains unclear. This study employs Phytolacca americana as study material to evaluate the impact of manganese (Mn) stress on the emission of volatile organic compounds (VOCs) and MIPVs. Additionally, we investigate whether the changes in MIPVs released by the emitter plants contribute to Mn tolerance in neighboring receiver plants. Our findings demonstrated that elevated levels of Mn stress significantly increased the total emissions and changed the compositions of MIPVs from plants cultivated in non-sterilized soil, in contrast to that of VOCs emitted by plants in sterilized soil. Moreover, MIPVs under high Mn stress conditions notably altered the hormonal profiles of the receiver plants, leading to a modification in their CSR strategies. Consequently, the receiver plants exhibited enhanced tolerance to subsequent Mn stress, as evidenced by improved growth performance, increased antioxidant enzyme activity, and reduced membrane damage. This study represents one of the initial efforts to elucidate the alerting effect of MIPVs induced by heavy metal stress on neighboring plants and its ecological implications.
Soil microbe-induced plant volatiles can alert neighboring plants for tolerating heavy metal stress
Under manganese stress, soil microorganisms induced changes in the volatile compounds of Phytolacca americana, triggering alerting effect in neighboring receiver plants. The production of MIPVs from emitter plants led receiver plants to shift their hormone state and ecological strategies, enhancing their tolerance to subsequent manganese stress.
Description of the Data and file structure
In this file, there are seven sheets, which include Experiments 1, 2, 3, and 4 measured in this study.
Experiment 1
In Experiment 1,there are two sheets, including concentration (ng/mL) of VOCs from emitter plants grown on sterilized soil and MIPVs of emitter plants grown on non-sterilized soil were listed. The column headers represent the CAS number of VOCs substance,VOCs that could not be retrieved are represented by unknown.
Experiment 2
In Experiment 2, there are two sheets, the leaf SA level (mg/g FW), the SA contents in root exudate(ng/ml), the leaf IAA level(ng/g FW), and CSR strategy scores(%) of receiver plants were listed in one sheet; soil bacterial OTU relative abundance (%) of MIPVs emitter and receiver plants were listed in the other one.
Experiment 3
In Experiment 3, the sheet includes dry weight (mg) (leaf, stem, root, and total) and CSR strategy scores(%) of receiver plants after Mn stress.
Experiment 4
In Experiment 4, there are two sheets, including tolerance indicators of VOCs receiver plants and MIPVs receiver plants before or after Mn stress.
The treatment codes are listed as below:
(1)Emitter plants:
Sterilized soil- inoculation with sterilized soil
Non-sterilized soil- inoculation with non-sterilized soil
e0mM-treatment with soil under non-Mn stress
e10mM-treatment with soil under 10mM Mn stress
e30mM-treatment with soil under 30mM Mn stress
e50mM-treatment with soil under 50mM Mn stress
(2)Receiver plants:
Sterilized soil- treatment with VOCs from emitter plants in sterilized soil
Non-sterilized soil- treatment with MIPVs from emitter plants in non-sterilized soil
R0mM-treatment with volatiles from emitter plants under non-Mn stress
R10mM-treatment with volatiles from emitter plants under 10mM Mn stress
R30mM-treatment with volatiles from emitter plants under 30mM Mn stress
R50mM-treatment with volatiles from emitter plants under 50mM Mn stress
Abbreviations used as below:
VOCs-volatile organic compounds
MIPVs-microbe-induced plant volatiles
SA-salicylic acid
IAA-indole-3-acetic acid
OTU-operational taxonomic units
CSR-competitiveness (C), stress tolerance (S) and ruderality (R)
CAT-catalase
POD-peroxidase
SOD-superoxide dismutase
H2O2-hydrogen peroxide
MG-methylglyoxal
MDA-malondialdehyde
Sharing/access Information
Links to other publicly accessible locations of the data: No
Was data derived from another source? No