Data from: Cardiomyocyte mechanodynamics under conditions of actin remodelling
Data files
Oct 16, 2019 version files 15.86 MB
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control 3 - M1.fcs
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Figure1_RT_DC_M1_data.tdms
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Figure2_AFM_Comparison.csv
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Figure2_AFM_Histogram.dat
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Figure2_Contractility_Data.txt
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Figure2_RT_DC_CytoD1_0_01_M1_data.tdms
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Figure2_RT_DC_CytoD1_0_1_M1_data.tdms
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Figure2_RT_DC_CytoD1_1_M1_data.tdms
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Figure2_RT_DC_CytoD1_10_M1_data.tdms
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Figure2_RT_DC_CytoD2_0_01_M1_data.tdms
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Figure2_RT_DC_CytoD2_0_1_M1_data.tdms
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Figure2_RT_DC_CytoD2_1_M1_data.tdms
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Figure2_RT_DC_CytoD2_10_M1_data.tdms
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Figure2_RT_DC_DMSO1_M1_data.tdms
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Figure2_RT_DC_DMSO2_M1_data.tdms
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sample 1uL - M1.fcs
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Abstract
The mechanical performance of cardiomyocytes is an important indicator of their maturation state and of
primary importance for the development of therapies based on cardiac stem-cells. As the mechanical analysis
of adherent cells at high-throughput remains challenging, we explore the applicability of real-time
deformability cytometry (RT-DC) to probe cardiomyocytes in suspension. RT-DC is a microfluidic
technology allowing for real-time mechanical analysis of thousands of cells with a throughput exceeding 1,000
cells per second. For cardiomyocytes derived from human induced pluripotent stem cells, we determined a
Young’s modulus of 1.250.08 kPa which is in close range to previous reports. Upon challenging the
cytoskeleton with cytochalasin D (CytoD) to induce filamentous actin depolymerization, we distinguish three
different regimes in cellular elasticity. Transitions are observed below 10 nM and above 103 nM and are
characterized by a decrease in the Young’s modulus. These regimes can be linked to cytoskeletal and
sarcomeric actin contributions by cardiomyocyte contractility measurements at varying CytoD concentrations,
where we observe a significant reduction in pulse duration only above 103 nM while no change is found for
compound exposure at lower concentrations. Comparing our results to mechanical cell measurements using
atomic force microscopy we demonstrate for the first time the feasibility of using a microfluidic technique to
measure mechanical properties of large samples of adherent cells while linking our results to the composition
of the cytoskeletal network.