Data from: Periaqueductal gray activates antipredatory neural responses in the amygdala of foraging rats
Data files
Oct 04, 2024 version files 1.91 MB
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Cross-correlation_dPAG_stim_BLA_recording_with_robot_session.xlsx
355.36 KB
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dPAG_stim_BLA_recording_with_robot_session.xlsx
353.42 KB
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dPAG_stim_BLA_recording.xlsx
443.49 KB
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Midline_thalamus_cfos.xlsx
12.98 KB
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Opto_tracing_Behavioral_data.xlsx
26.37 KB
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PAG_single_units.xlsx
711.62 KB
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README.md
5.70 KB
Abstract
Pavlovian fear conditioning research suggests that the interaction between the dorsal periaqueductal gray (dPAG) and basolateral amygdala (BLA) acts as a prediction error mechanism in the formation of associative fear memories. However, their roles in responding to naturalistic predatory threats, characterized by less explicit cues and the absence of reiterative trial-and-error learning events, remain unexplored. In this study, we conducted single-unit recordings in rats during an ‘approach food-avoid predator’ task, focusing on the responsiveness of dPAG and BLA neurons to a rapidly approaching robot predator. Optogenetic stimulation of the dPAG triggered fleeing behaviors and increased BLA activity in naive rats. Notably, BLA neurons activated by dPAG stimulation displayed immediate responses to the robot, demonstrating heightened synchronous activity compared to BLA neurons that did not respond to dPAG stimulation. Additionally, the use of anterograde and retrograde tracer injections into the dPAG and BLA, respectively, coupled with c-Fos activation in response to predatory threats, indicates that the midline thalamus may play an intermediary role in innate antipredatory defensive functioning.
GENERAL INFORMATION
- Title of Dataset: dPAG-BLA connectivity
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Author Information
A. Principal Investigator Contact Information
Name: Jeansok J Kim
Institution: University of Washington
Address: Seattle, WA 98195
Email: jeansokk@u.washington.eduB. Co-investigator Contact Information
Name: Jeiwon Cho
Institution: Ewha Womans University
Address: Seoul, Korea
Email: jelectro21@ewha.ac.krC. First Author Contact Information
Name: Eun Joo Kim
Institution: University of Washington
Address: Seattle, WA 98195
Email: ejkim731@uw.edu - Date of data collection : 2016-02 ~ 2023-12
- Geographic location of data collection : University of Washington, Seattle, WA 98195
- Information about funding sources that supported the collection of the data: Foundation for the National Institutes of Health (MH099073, Jeansok J Kim; AG067008, Eun Joo Kim; F32MH127801, Mi-Seon Kong), National Research Foundation of Korea (NRF-2022M3E5E8018421, NRF-2022R1A2C2009265; Jeiwon Cho)
Raw data belonging to: Eun Joo Kim et al., (2024) Periaqueductal gray activates antipredatory neural response
DATA & FILE OVERVIEW
File List:
PAG_single units
dPAG-opto_behavior
dPAG stim_BLA recording
dPAG stim_BLA recording_with robot session
Midline thalamus_cfos
METHODOLOGICAL INFORMATION
Description of methods used for collection/generation of data:
Single-unit activity was recorded from the dorsal periaqueductal gray (dPAG) and basolateral amygdala (BLA). Peri-event time histogram and spike synchrony were computed and generated by using Neuroexplorer.
DATA-SPECIFIC INFORMATION FOR: [PAG_single units]
A total of 96 dPAG cells were recorded.
Tabs 1-3 (Firing rates): All 96 cells’ firing rates during pre-robot, robot, and post-robot sessions (-5 sec to 5 sec; 0.1-s bin; t=0, pellet procurement or robot activation).
Tab 4 (Z-scored dPAG activity): All 96 cells’ z-scores during pre-robot, robot, and post-robot sessions (-5 sec to 5 sec; 0.1-s bin; t=0, pellet procurement or robot activation).
Tab 5 (Cell types): Robot, pellet, and BOTH cells.
DATA-SPECIFIC INFORMATION FOR: [Opto_tracing_Behavioral data]
dPAG stimulation and foraging behaviors
Tab 1 (Baseline foraging): Pellet procurement latency for baseline foraging sessions (Day 1-4)
Variable information
ChR2: dPAG stimulation group
EYFP: control stimulation group
S: short pellet distance
M: medium pellet distance
L: long pellet distance
Tab 2 (Testing foraging): Pellet procurement latency for testing session
Stim day: testing (dPAG stimulation) composed of 3 baseline trials and dPAG stimulation trials (long and short distances)
Tab 3 (dPAG stimulation_intensity): Pellet procurement latency for testing session (variable intensities)
Tab 4 (dPAG stimulation_frequency): Pellet procurement latency for testing session (variable frequencies)
Tab 5 (dPAG stimulation_duration): Pellet procurement latency for testing session (variable durations)
Tab 6 (tracing animals): Pellet procurement latency for baseline and testing sessions with (Robot group) or withouth (Control group) a robot predator
DATA-SPECIFIC INFORMATION FOR: [Midline thalamus_cfos]
Fos-positive cell counting data
Tab 1 (cfos): numbers of fos-postive cells and % relative to control data
Con: foraging-only control group
Robot: robot-experienced group
PVT: paraventricular nucleus of the thalamus
IMD: intermediate thalamus
CM: central medial thalamus
Rh: rhomboid nuclei of the thalamus
Re: reuniens of the thalamus.
DATA-SPECIFIC INFORMATION FOR: [dPAG stim_BLA recording]
A total of 328 BLA cells were recorded.
Tabs 1-3 (Firing rates): All 321 cells’ firing rates during pre-stim, stim, and post-stim sessions (-5 sec to 5 sec; 0.1-s bin; t=0, pellet procurement or robot activation).
Tab 4 (Z-scored BLA activity): dPAG stimulation-excited cells
Tab 5 (Z-scored BLA activity): dPAG stimulation-inhibited cells
DATA-SPECIFIC INFORMATION FOR: [dPAG stim_BLA recording_with robot session]
A total of 85 BLA cells were analyzed.
Tabs 1-4 (Firing rates): All 85 cells’ firing rates during pre-stim, stim, post-stim, and robot sessions (-5 sec to 5 sec; 0.1-s bin; t=0, pellet procurement or robot activation).
Tab 5 (Z-scored BLA activity_Stim cells data): Robot responsivenss of the dPAG stimulation-excited cells. Cell types are classified in rows 408 and 409 (Stim cells, Robot cells, and nonRobot cells)
Tab 6 (Z-scored BLA activity_nonStim cells data): Robot responsivenss of the dPAG stimulation non-responsive cells. Cell types are classified in rows 408 and 409 (nonStim cells, Robot cells, and nonRobot cells)
Tab 7 (Z-scored BLA activity_inhibited cell): A dPAG stimulation-inhibited cell
DATA-SPECIFIC INFORMATION FOR: [Cross-correlation_dPAG stim_BLA recording_with robot session]
Cross-correlation data from the BLA cell pairs that showed significant synchrony during the robot encounters.
Tab 1 (CC_significant pairs): Firing rates of BLA cells relative to paired BLA cells (using shift predictors with 100 random trial shuffles).
Tab 2 (Z scored CC_significant pairs): Z-scored firings of BLA cells relative to paired BLA cells. The X-axis data of the purple columns have been reversed based on the CC peak positions (negative values).
Tab 3 (Stim vs nonStim pairs): Z-scored firings of BLA cells relative to paired BLA cells for Stim pairs (contained at least one of the stimulation-responsive units) vs nonStim pairs (included only stimulation-nonresponsive units).
Missing data code: NA
The dataset was collected by performing single-unit recording, optogenetics, and tracing techniques. All data were processed using Neuroexploer and customed Matlab programs.