Data from: Cell shape and the microenvironment regulate nuclear translocation of NF-kappaB in breast epithelial and tumor cells
Data files
Jan 16, 2016 version files 3.66 GB
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AG1573 fibroblast.zip
142.28 MB
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AU565.zip
143.44 MB
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BT474.zip
115.17 MB
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CAMA1.zip
122.15 MB
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HCC1143.zip
202.14 MB
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HCC1954.zip
130.17 MB
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HCC70.zip
125.12 MB
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HeLa.zip
198.70 MB
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Hep3B.zip
224.61 MB
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hs578T.zip
196.80 MB
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JIMT1.zip
203.44 MB
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MCF10A-no EGF.zip
146.27 MB
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MCF12A-no EGF.zip
206.12 MB
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MCF7.zip
188.39 MB
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MDA-MB-157.zip
137.38 MB
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MDA-MB-231.zip
168.68 MB
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MDA-MB-453.zip
135.63 MB
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README_for_AG1573 fibroblast.rtf
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README_for_AU565.rtf
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README_for_BT474.rtf
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README_for_CAMA1.rtf
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README_for_HCC1143.rtf
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README_for_HCC1954.rtf
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README_for_HCC70.rtf
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README_for_HeLa.rtf
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README_for_Hep3B.rtf
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README_for_hs578T.rtf
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README_for_JIMT1.rtf
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README_for_MCF10A-no EGF.rtf
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README_for_MCF12A-no EGF.rtf
35.52 KB
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README_for_MCF7.rtf
35.52 KB
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README_for_MDA-MB-157.rtf
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README_for_MDA-MB-231.rtf
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README_for_MDA-MB-453.rtf
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README_for_SKBR3.rtf
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README_for_SUM149.rtf
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README_for_SUM159.rtf
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README_for_T47D.rtf
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README_for_ZR75.1.rtf
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SKBR3.zip
158.17 MB
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SUM149.zip
161.44 MB
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SUM159.zip
261.58 MB
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T47D.zip
160.24 MB
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ZR75.1.zip
126.64 MB
Abstract
Although a great deal is known about the signaling events that promote nuclear translocation of NF‐κB, how cellular biophysics and the microenvironment might regulate the dynamics of this pathway is poorly understood. In this study, we used high‐content image analysis and Bayesian network modeling to ask whether cell shape and context features influence NF‐κB activation using the inherent variability present in unperturbed populations of breast tumor and non‐tumor cell lines. Cell–cell contact, cell and nuclear area, and protrusiveness all contributed to variability in NF‐κB localization in the absence and presence of TNFα. Higher levels of nuclear NF‐κB were associated with mesenchymal‐like versus epithelial‐like morphologies, and RhoA‐ROCK‐myosin II signaling was critical for mediating shape‐based differences in NF‐κB localization and oscillations. Thus, mechanical factors such as cell shape and the microenvironment can influence NF‐κB signaling and may in part explain how different phenotypic outcomes can arise from the same chemical cues.