Data from: Sexual conflict and social networks in bed bugs: Effects of social experience
Data files
Oct 21, 2024 version files 128.89 KB
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exp_1_assoc_mat_r1.csv
1.67 KB
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exp_1_assoc_mat_r2.csv
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exp_1_assoc_mat_r3.csv
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exp_1_assoc_mat_r4.csv
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exp_1_assoc_mat_r5.csv
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exp_1_assoc_mat_r6.csv
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exp_1_combined_individual_data.csv
13 KB
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exp_1_main_analyses.R
6.45 KB
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exp_1_network_analysis.R
4.39 KB
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exp_1_oppo_sex_network_analysis.R
2.51 KB
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exp_1_permutation_test.R
4.47 KB
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exp_1_raw_aggregations.csv
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exp_2_all_male_analysis.R
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exp_2_assoc_mat_r1.csv
856 B
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exp_2_assoc_mat_r2.csv
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exp_2_assoc_mat_r3.csv
806 B
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exp_2_assoc_mat_r4.csv
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exp_2_assoc_mat_r5.csv
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exp_2_assoc_mat_r6.csv
991 B
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exp_2_male_summary_data.csv
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exp_2_network_analysis.R
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exp_2_opposite_sex_network_analysis.R
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exp_2_permutation_test_males.R
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exp_2_raw_aggregations.csv
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exp_3_aggregations_by_shelter_fems.csv
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exp_3_all_fem_analysis.R
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exp_3_assoc_mat_r1_fem.csv
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exp_3_assoc_mat_r2_fem.csv
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exp_3_assoc_mat_r3_fem.csv
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exp_3_assoc_mat_r4_fem.csv
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exp_3_assoc_mat_r5_fem.csv
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exp_3_assoc_mat_r6_fem.csv
818 B
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exp_3_fem_oppo_sex_networks.R
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exp_3_fem_summary_data.csv
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exp_3_permutation_test_females.R
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exp_3_social_network_analysis.R
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README.md
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Abstract
Living in groups can provide essential experience that improves sexual performance and reproductive success. While the effects of social experience have drawn considerable scientific interest, commonly used behavioural assays often do not capture the dynamic nature of interactions within a social group. Here, we conducted three experiments using a social network framework to test whether social experience during early adulthood improves the sexual competence of bed bugs (Cimex lectularius) when placed in a complex and competitive group environment. In each experiment, we observed replicate groups of bed bugs comprising previously socialized and previously isolated individuals of the same sex, along with an equal number of standardized individuals of the opposite sex. Regardless of whether we controlled for their insemination history, previously isolated males mounted and inseminated females at significantly higher rates than previously socialized males. However, we found no evidence of social experience influencing our other measures of sexual competence: proportion of mounts directed at females, ability to overcome female resistance, and strength of opposite-sex social associations. We similarly did not detect effects of social experience on our female sexual competence metrics: propensity to avoid mounts, rate of successfully avoiding mounts, opposite-sex social association strength, and rate of receiving inseminations. Our findings indicate that early social experience does not improve sexual competence in male and female bed bugs.
Experiment 1
The code used to analyze and visualize the major figures and results can be found in the “exp_1_main_analyses.R” file. The data used for this file is “exp_1_combined_individual_data.csv”. Each column in this file includes data for a single individual from a single replicate.
Below are definitions of variables that may not be self-explanatory:
ID: Unique ID given to a specific male
male_mounts: Number of mounts directed at other males
female_mounts: Number of mounts directed at females
mounts_evaded: Number of mounts performed where females successfully avoided
attempt_avoid: Number of mounts performed where females attempted to avoid
mounts_aborted: Number of female mounts that the male aborted
mounts_choice: Number of female mounts where males had the opportunity to abort
abort_rate: Proportion of mounts aborted where males had the choice to abort
avoid_success_rate: Number of mounts females successfully avoided divided by number of mounts females attempted to avoid
prop_male: Proportion of mounts directed at other males instead of females
oppo_sex_strength: Opposite sex social network association strength (obtained from social network analyses)
All the other exp_1 files are for the opposite-sex network analysis. The raw data is in the form of a list of all individuals participating in every aggregation identified during hourly scans in a file called “exp_1_raw_aggreations.csv”. The individuals in each aggregation are listed in the “Members” column where each letter ID represents an individual bed bug. We converted number IDs to letter IDs because csv files do not handle the . and : symbols well. The R script named “exp_1_network_analysis.R” converts this list of aggregations into association matrices which are all also uploaded here. We then removed all same-sex association values from these association matrices so that they could be converted into opposite-sex networks (social association networks that only depict opposite sex associations). These matrix files have a blank cell in the first row/column because that’s the default structure of association matrices.
The “exp_1_oppo_sex_network_analysis.R” file generates opposite sex social networks while the “exp_1_permutation_test.R” file contains code used to run the node-based permutation test for this experiment.
In general, it may be necessary to add “exp_1” to the names of csv files to get the R scripts to run properly as file names were modified after analysis, prior to uploading for ease of navigation on DRYAD.
Experiments 2 and 3
Follow the same instructions as for Experiment 1 except replace exp_1 with exp_2/3. Some file names are slightly different (for example, the R scripts with the majority of the relevant analyses are named “exp_2_all_male_analysis.R” and “exp_3_all_fem_analysis.R”). Lastly, like exp_1 again, file names may have to be changed slightly in the read.csv step of the R script, usually by adding “exp_x” in front of the file name to get things to run.
exp_2_male_summary_data.csv variable definitions (data file for main analyses in Experiment 2):
male_mounts: Number of mounts directed at other males
female_mounts: Number of mounts directed at females
attempt_avoid: Number of mounts performed where females attempted to avoid
success_avoid: Number of mounts performed where females successfully avoided
aborts: Number of mounts directed at females aborted by males
possible_aborts: Number of mounts directed at females where males had the choice to abort
exp_3_female_summary_data.csv variable definitions (data file for main analyses in Experiment 3):
inseminations: Number of times a focal female was inseminated
mounts: Number of times a focal female was mounted
attempt_avoid: Number of mounts a female attempted to avoid
success_avoid: Number of mounts a female successfully avoided
prop_avoid: Proportion of total mounts a female successfully avoided
avoid_success: Proportion of mounts females attempted to avoid where she successfully avoided
oppo_sex_strength: Opposite sex social network association strength (obtained from social network analyses)
Missing data code: NA
Sexual interactions and outcome data for each replicate within each of the three experiments was collected through live continous observations of individually marked bed bugs. In addition to continous live observations of sexual interactions, we also examined bed bugs’ social associations by performing scans where we documented the location of each bed bug. The list of aggregating individuals was translated into association matrices to generate opposite-sex social networks.