Four .csv data files

1. floralMorphology

This dataset includes floral measurements from ~6 flowers/plant in a resurrection experiment done with Ipomoea purpurea (the common morning glory).
Seeds were collected from 15 populations in the SE United States at two time points, 2003 and 2012, and grown together in a common garden greenhouse experiment.
Floral measurements included are corolla width, corolla length, and the distance between the tallest anther and stigma.
Results show directional selection for increased corolla width over time, no change in corolla length over time, and balancing selection on anther-stigma distance.
Other information in the datasheet includes a population ID number, name, and location (latitude and longitude) for each population. Further results indicate differential selection across latitudes.

2. phenology

This dataset includes phenology measurements from a resurrection experiment done with Ipomoea purpurea (the common morning glory).
Seeds were collected from 23 populations in the SE United States at two time points, 2003 and 2012, and grown together in a common garden greenhouse experiment.
The recorded phenological trait is the date of first flower. Dates have been converted to Julian Date and all seeds were planted on the same day, so this date is representative of the number of days between planting and flowering
Results show some evidence of directional selection for earlier flowering dates.
Other information in the datasheet includes a population ID number, name, and location (latitude and longitude) for each population. Further results indicate differential selection across latitudes.

3. nectarBrix

This dataset includes measurements of sugar levels in nectar (Brix scale) from 6-7 flowers/plant in a resurrection experiment done with Ipomoea purpurea (the common morning glory).
Seeds were collected from 4 populations in the SE United States at two time points, 2003 and 2012, and grown together in a common garden greenhouse experiment.
Results demonstrate some increase in sugar content over time, particularly at northern latitudes, but this result is highly sensitive to population removal due to low sampling.
Other information in the datasheet includes a population ID number, name, and location (latitude and longitude) for each population.

4. pollenCount

This dataset includes a count of pollen grains from 6-7 flowers/plant in a resurrection experiment done with Ipomoea purpurea (the common morning glory).
Seeds were collected from 4 populations in the SE United States at two time points, 2003 and 2012, and grown together in a common garden greenhouse experiment.
Results show a non-significant trend toward increased pollen counts over time.
Other information in the datasheet includes a population ID number, name, and location (latitude and longitude) for each population.

Description of the data and file structure

Each datasheet represents a separate greenhouse resurrection experiment that was conducted to measure: 1. floral morphology, 2. phenology, and 3. floral reward traits (split into two datasheets - one for nectar, and one for pollen counts).
Measurements of floral morphology traits are contained in a .csv file of that name “floralMorphology.csv”. Timing of first flower data is recorded in the “phenology.csv” file, and floral reward traits are represented in the “nectarBrix.csv” and “pollenCount.csv” files

Population ID number/name are shared across all data sheets (i.e. Population 1 in the floralMorphology spreadsheet is the same population as Population 1 in phenology, nectarBrix, and pollenCount).

floralMorphology Columns:

• PopID - ID number assigned to each unique, naturally occurring, population from which seeds were collected; maps to Population column in the phenology file and to pop.id in the nectarBrix and pollenCount files
• Date - date a measurement was taken in the greenhouse
• IDNumber - ID number assigned to each individual greenhouse plant measured in the resurrection experiment; unique to this experiment & does NOT map to any other datasheet
• Year - year (either 2003 or 2012) in which seeds were collected from naturally occurring populations
• TA - length of tallest anther; measurement (in mm) from the base of the sepal to the tip of the tallest anther
• Stigma - length of the stigma; measurement (in mm) from the base of the sepal to the tip of the stigma
• CorLength - corolla length; measurement (in mm) from the base of the sepal to the outer edge of the corolla
• CorWidth - corolla width; measurement (in mm) of the diameter of the corolla
• FLColor - flower color for a given plant ID; PU = Purple; PI = Pink; WH = White
• ASD - anther-stigma distance; difference between the length of the tallest anther and the stigma, calculated as Stigma - TA for each row
• PopulationName - name assigned to each unique, naturally occurring, population from which seeds were collected; corresponds 1:1 with PopID
• FieldType - all populations from which seeds were collected occurred adjacent to agricultural fields; FieldType indicates the type of agricultural use for each population (either corn or soy)
• Lat - latitude for each population
• Long - longitude for each population

phenology Columns:

• Population - ID number assigned to each unique, naturally occurring, population from which seeds were collected; maps to PopID column in the floralMorphology file and to pop.id in the nectarBrix and pollenCount files
• PlantNumber - ID number assigned to each individual greenhouse plant measured in the resurrection experiment; unique to this experiment & does NOT map to any other datasheet
• Year - year (either 2003 or 2012) in which seeds were collected from naturally occurring populations
• YearShort - abbreviation for each year where 3 = 2003 and 12 = 2012
• Day - day of the month in which the first flower on a plant opened (taken from the Date column)
• Month - month in which the first flower on a plant opened (taken from the Date column)
• Date - date on which the first blooms emerged on a plant (date of first flower)
• Julian - date of first flower converted into Julian Date
• PopulationName - name assigned to each unique, naturally occurring, population from which seeds were collected; corresponds 1:1 with PopID
• FieldType - all populations from which seeds were collected occurred adjacent to agricultural fields; FieldType indicates the type of agricultural use for each population (either corn or soy)
• Lat - latitude for each population
• Long - longitude for each population

nectarBrix Columns:

• pop.id - ID number assigned to each unique, naturally occurring, population from which seeds were collected; maps to Population column in the phenology file, PopID in the floralMorphology file, and pop.id in the pollenCount file
• plant_number - ID number assigned to each individual greenhouse plant measured in the resurrection experiment; unique to this experiment & does NOT map to any other datasheet
• Brix - measurement of the sugar content of the nectar in a flower using the Brix scale and recorded from a pocket refractometer
• maternal_line - ID assigned to each maternal line represented from field collections; maps to maternal_line in pollenCount file_
• individual - within each unique combination of population, maternal line, and year, multiple individual plants and multiple flowers/plant are measured. Each row is a flower, with the “individual” column noting which rows are flowers from the same plant
• year - year (either 2003 or 2012) in which seeds were collected from naturally occurring populations
• flower.color - flower color for a given plant ID; flower color was only collected for a subset of plants, so missing data is marked with “na”.
• PopulationName - name assigned to each unique, naturally occurring, population from which seeds were collected; corresponds 1:1 with PopID
• FieldType - all populations from which seeds were collected occurred adjacent to agricultural fields; FieldType indicates the type of agricultural use for each population (either corn or soy)
• Lat - latitude for each population
• Long - longitude for each population

pollenCount Columns:

• pop.id - ID number assigned to each unique, naturally occurring, population from which seeds were collected; maps to Population column in the phenology file, PopID in the floralMorphology file, and pop.id in the nectarBrix file
• year - year (either 2003 or 2012) in which seeds were collected from naturally occurring populations
• maternal_line - ID assigned to each maternal line represented from field collections; maps to maternal_line in the nectarBrix file_
• pollen_slide - number before the decimal is the plant from which pollen was collected; number after the decimal is the flower on the plant (i.e. slide 1.1 is plant 1, flower 1; slide 1.2 is plant 1, flower 2)
• pollen_count - number of pollen grains in a flower calculated using imageJ software from images of slides prepared with the 2nd tallest anther & fuchsin cube jelly
• PopulationName - name assigned to each unique, naturally occurring, population from which seeds were collected; corresponds 1:1 with PopID
• FieldType - all populations from which seeds were collected occurred adjacent to agricultural fields; FieldType indicates the type of agricultural use for each population (either corn or soy)
• Lat - latitude for each population
• Long - longitude for each population

Code/Software

All analysis and generation of figures included as an R markdown file. Two external R scripts are referenced and included.