This dataset contains offspring genotypes, Cuticular hydrocarbon calls for all parents and supplementary individuals, allele frequencies used in the Colony program, and results from the Colony program

Description of the data and file structure

There are five data sheets:

Allele_Frequencies.xlsx: These are allele frequencies used in the Colony analyses for each brood. The allele frequencies were calculated in CERVUS and used as input “known allele frequencies” to increase COLONY’s accuracy

COLONY_Output.xlsx: Results from Colony run. Each species was analyzed with 3 different error rates (each in a separate tab), fathers for each female brood (Female ID) are designated with numbers. The "Prob (Inc.) refers to the probability that all individuals of a given fullsib family are fullsibs - the lower the probability the higher the change that the fullsibship could be slit. The "Prob (exc.) is the probability that no other individuals are fullsibs to this family (See Colony for more information). The #offspring/father is a count of offspring assigned to a particular father. The following list (AssignedO1...) refer to the offspring_ID assigned to a particular father in each brood.

Offspring_Genotype_(1).xlsx: These are the microsatellite calls for each offspring. Each individual is represented by one row and each locus is represented by two columns. Loci names are in the first row, two columns for each (allele 1 and allele 2). Loci colors for each locus represent the fluorescent dye color used for genotyping (see paper for more information)

Mother_Genotypes.xlsx: These are the microsatellite calls for each brood mother. Each individual is represented by one row and each locus is represented by two columns. Loci names are in the first row, two columns for each (allele 1 and allele 2). Loci colors for each locus represent the fluorescent dye color used for genotyping (see paper for more information)

Fertility_Count.xlsx: A count of total offspring for each female. One tab for Gryllus firmus females and one tab for Gryllus pennsylvanicus females

CHCPCA2023.csv: Sample is the sample ID: GFF (and F) represent Gryllus firmus females, GFM represent Gryllus firmus males, GPF (and P) represent Gryllus pennsylvanicus females, GPM represent Gryllus pennsylvanicus males. Results from Masshunter Qualitative Analysis Version B.10, showing the top 20 peak areas within each sample. Peak refers to peaks observed in a particular individual chromatogram, RT is retention time (Time taken for a compound to pass through the GC column), Area is the total area under the peak and Area % is the percentage of the total area attributed to a specific peak, indicating relative abundance, Height is the maximum intensity of the peak, reflecting the compound's concentration and Width of the peaks. Max Y: Highest y-axis value of the peak, related to its intensity. Symmetry: Measure of the peak's symmetry, affecting data quality. Tailing Factor: Ratio of the peak's trailing to leading edges, assessing peak shape.  FWHM (Full Width at Half Maximum) indicates how wide a peak is at its midpoint. The Reference point indicates which peak is the docosane peak.  Species and Sex are indicated on the final two columns.

And scripts related to R analyses and classification of females based on the analyses

01_CHC_Alignment_Manual.R: This is the R script used for the alignment and analyses of the CHC data (CHCPCA2003.csv). 

02_Fertility.R: This is the R script used for the analyses of the fertility data (Fertility_Count.xlsx) 

03_Paternity.R: This is the R script used for the analyses of the Paternity data (COLONY_Output.xlsx) 

MLLabeled.csv: list of females classified according to the PCA analyses (see paper for details), females with a male-like CHC profile are listed with ML.

Sharing/Access information

Data was derived from the following sources:
Microsatellite genotypes for 7 loci, Cuticular hydrocarbon collections for individual crickets using GCMS (see paper for details)

Code/Software (uploaded as "data")

The R code used in the analyses