Data from: Deciphering a survival strategy during the interspecific competition between Bacillus cereus MSM-S1 and Pseudomonas sp. MSM-M1
Data files
Oct 20, 2016 version files 2.18 MB
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Comparative growth characteristics of S1 on Nutrient agar plate.xlsx
22.91 KB
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Comparative growth pattern of M1 on Nutrient agar plate.xls
55.30 KB
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Competitive growth patterns of S1 and M1 in Nutrient broth.xls
48.64 KB
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File S1_supporting Information_BCetal.doc
1.94 MB
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Growth Characteristics of S1 and M1 in Nutrient Broth.xls
59.90 KB
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Growth characteristics of S1 and M1 on Nutrient agar plates.xlsx
25.14 KB
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README_for_Growth Characteristics of S1 and M1 in Nutrient Broth.xlsx
25.14 KB
Abstract
Interspecific competition in bacteria governs colony growth dynamics and pattern formation. Here, we demonstrate an interesting phenomenon of interspecific competition between Bacillus cereus MSM-S1 and Pseudomonas sp. MSM-M1, where secretion of an inhibitor by Pseudomonas sp. is used as a strategy for survival. Although B. cereus grows faster than Pseudomonas sp., in the presence of Pseudomonas sp. the population of B. cereus reduces significantly, whereas Pseudomonas sp. do not show any marked alteration in their population growth. Appearance of a zone of inhibition between growing colonies of two species on nutrient agar prevents the expanding front of the MSM-S1 colony from accessing and depleting nutrients in the region occupied by MSM-M1, thereby aiding the survival of the slower growing MSM-M1 colonies. To support our experimental results, we present simulations, based on a chemotactic model of colony growth dynamics. We demonstrate that the chemical(s) secreted by Pseudomonas sp. is responsible for the observed inhibition of growth and spatial pattern of the B. cereus MSM-S1 colony. Our experimental results are in excellent agreement with the numerical results and confirm that secreted inhibitors enable Pseudomonas sp. to survive and coexist in the presence of faster growing B. cereus, in a common niche.