Sublethal effects of Voliam Flexi on Spodoptera frugiperda (Lepidoptera: Noctuidae): Insights from Life Table Theory
Data files
Mar 12, 2025 version files 95.86 KB
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Development_data.xlsx
22.01 KB
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Female_fecundity.xlsx
14.26 KB
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Gulu_Data__(1).xlsx
25.28 KB
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Life_table_data.xlsx
28.71 KB
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README.md
5.59 KB
Abstract
Fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae) is a significant insect pest causing huge economic losses to maize crops and has attracted attention globally in the last decade. The study on sublethal effect of insecticide is crucial for the management of insect pests in the field. Thus, the current study evaluated the sublethal effects of Voliam Flexi, which is a combination of Thiamethoxam and Chlorantraniliprole, on the demographic parameters of S. frugiperda exposed to LC10, LC20 and LC30 concentrations. The results indicated that S. frugiperda larvae showed longer period (35.4 days at LC10, 37.9 days at LC20, and 39.5 days at LC30) compared to those in the control group (30.4 days). Fecundity rate was also reduced when sublethal concentrations were applied (298.1 eggs at LC10, 253.2 eggs at LC20, and 214.1 eggs at LC30), when compared with the control group (339.3 eggs). The larvae exposed to sublethal doses exhibited a significant decrease in the intrinsic rate of increase (r), net reproduction rate (R₀), and finite rate of increase (λ) compared to the control group. These findings suggest that sublethal concentrations of Voliam Flexi adversely affect the growth and reproductive capacity of S. frugiperda, ultimately aiming to keep populations below levels that cause economic damage. Our findings highlight the potential of sublethal concentration of Voliam Flexi as an effective and alternative for insecticide resistance management strategies.
https://doi.org/10.5061/dryad.vmcvdnd37
Description of the data and file structure
README File for Dataset: Sublethal Effects of Voliam Flexi on Spodoptera frugiperda (Lepidoptera: Noctuidae)
DOI: https://doi.org/10.5061/dryad.vmcvdnd37
1. Overview of the Dataset
This dataset contains life table data from an experiment investigating the sublethal effects of Voliam Flexi on the fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae). The data includes developmental stages, survival rates, and fecundity of the insects under different treatment conditions. The goal of the study was to understand how sublethal doses of Voliam Flexi impact the life history traits of Spodoptera frugiperda.
2. File Structure
The dataset is provided in an Excel file named "Life table data.xlsx". The file contains one sheet titled "Life table data", which includes the following columns:
Columns in the Excel File:
- TRT: Treatment group (e.g., LC10, LC20, LC30, Control).
- Replication: Replicate number for each treatment group.
- Egg LAYING: Number of eggs laid by the female.
- 1st instar: Number of insects that reached the 1st instar stage.
- 2nd instar: Number of insects that reached the 2nd instar stage.
- 3rd instar: Number of insects that reached the 3rd instar stage.
- 4th instar: Number of insects that reached the 4th instar stage.
- 5th instar: Number of insects that reached the 5th instar stage.
- 6th instar: Number of insects that reached the 6th instar stage.
- PUPAE: Number of insects that reached the pupal stage.
- ADULT: Number of insects that reached the adult stage.
- SEX RATIO: Sex of the adult insects (F = Female, M = Male, NA = Not Applicable/Dead).
- TRT (repeated): Treatment group repeated for fecundity data.
- Day 1 to Day 14: Daily fecundity data (number of eggs laid by female adults on each day).
3. Explanation of Terms and Abbreviations
- TRT: Treatment groups, where:
- LC10: Sublethal concentration 10%.
- LC20: Sublethal concentration 20%.
- LC30: Sublethal concentration 30%.
- Control: No treatment (control group).
- Replication: Each treatment group was replicated multiple times to ensure statistical reliability. The number after "R" indicates the replicate number (e.g., R1, R2, etc.).
- Instar Stages: Insects go through several larval stages (instars) before reaching adulthood. The numbers in these columns indicate how many insects survived to each stage.
- PUPAE: The pupal stage is the non-feeding, transformative stage between larva and adult.
- ADULT: The final stage of the insect's life cycle, where they are sexually mature and capable of reproduction.
- SEX RATIO: Indicates the sex of the adult insects:
- F: Female.
- M: Male.
- NA: Not Applicable (insects died before reaching adulthood or data was not recorded).
- N/A: This abbreviation is used in the dataset to indicate missing or unavailable data. Specifically:
- If an insect died before reaching a particular stage, the cell is marked as N/A.
- If no data was recorded for a particular day or stage, the cell is also marked as N/A.
4. Experimental Design
The experiment was designed to study the sublethal effects of Voliam Flexi on Spodoptera frugiperda. Insects were exposed to different sublethal concentrations (LC10, LC20, LC30) of Voliam Flexi, and a control group was maintained without any treatment. The following parameters were recorded:
- Developmental Stages: The number of insects surviving to each developmental stage (1st instar to adult) was recorded.
- Fecundity: The number of eggs laid by female adults was recorded daily for 14 days.
- Sex Ratio: The sex of the adult insects was recorded to analyze any sex-specific effects of the treatment.
5. Data Analysis
The data was analyzed using Life Table Theory, which is a demographic tool used to study the survival and reproduction of populations. The following analyses were performed:
- Survival Rates: The proportion of insects surviving to each developmental stage was calculated.
- Fecundity: The total number of eggs laid by female adults was summed across the 14-day period.
- Sex Ratio: The ratio of male to female adults was calculated for each treatment group.
6. Notes on Missing Data
- N/A: Cells marked as N/A indicate that the insect died before reaching that particular stage, or no data was recorded for that day/stage. This is important for understanding the survival rates and developmental success of the insects under different treatments.
- Empty Cells: Some cells may be left empty if no data was recorded. These are not to be confused with N/A values, which specifically indicate mortality or lack of data.
7. Code/Software Used
The data was analyzed using MSChart and other statistical software for life table analysis.
8. Contact Information
For any questions or further clarification regarding this dataset, please contact the corresponding author at (yds_agr@shzu.edu.cn).
Access information
Other publicly accessible locations of the data:
- Journal of Applied Entomology
Data was derived from the following sources:
- Original Resarch
Life Table theory