In this study, we investigated the impact of domestic and wild prey availability on snow leopard prey preference in the Kangchenjunga Conservation Area of eastern Nepal—a region where small domestic livestock are absent and small wild ungulate prey is present. We took a comprehensive approach that combined fecal genetic sampling, macro- and microscopic analyses of snow leopard diets, and direct observation of blue sheep and livestock in the KCA. Out of the collected 88 putative snow leopard scat samples from 140 transects (290 km) in 27 (4 × 4 km²) sampling grid cells, 73 (83%) were confirmed to be from snow leopard. The genetic analysis accounted for 19 individual snow leopards (10 males and 9 females), with a mean population size estimate of 24 (95% CI: 19–29) and an average density of 3.9 snow leopards/100 km² within 609 km². The total available prey biomass of blue sheep and yak was estimated at 355,236 kg (505 kg yak/km² and 78 kg blue sheep/km²). From the available prey biomass, we estimated snow leopards consumed 7% annually, which comprised wild prey (49%), domestic livestock (45%), and 6% unidentified items. The estimated 47,736 kg blue sheep biomass gives a snow leopard-to-blue sheep ratio of 1:59 on a weight basis. The high preference for snow leopards to domestic livestock appears to be influenced by a much smaller available biomass of wild prey than in other regions of Nepal (e.g., 78 kg/km² in the KCA compared with a range of 200–300 kg/km² in other regions of Nepal). Along with livestock insurance scheme improvement, there needs to be a focus on improved livestock guarding, and predator-proof corrals as well as engaging and educating local people to be citizen scientists on the importance of snow leopard conservation, involving them in long-term monitoring programs and promotion of ecotourism.

Scat samples were collected from the natural habitat of snow leopards in defined sampling grids and transects. 

The collected samples were processed for identification of species, sex, and individuals. Genetic analyses (DNA extraction, species identification, sex identification, and individual identification) of putative snow leopard scat samples were conducted following the protocol developed by Karmacharya et al., 2011, adopted from Janecka et al. (2008).