Data from: Particle-associated bacterioplankton communities across the Red Sea
Data files
Mar 27, 2025 version files 41.35 MB
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clean_BPBN.csv
41.35 MB
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README.md
1.60 KB
Abstract
Pelagic particle-associated bacterioplankton play crucial roles in marine ecosystems, influencing biogeochemical cycling and ecosystem functioning. However, their diversity, composition, and dynamics remain poorly understood, particularly in unique environments such as the Red Sea. In this study, we employed eDNA metabarcoding to comprehensively characterise bacterioplankton communities associated with pelagic particles in a three-dimensional assessment spanning depths from the surface to up to 2300 m along the full length of the eastern Red Sea within the exclusive economic zone of the Kingdom of Saudi Arabia. Our results reveal a diverse assemblage of taxa, with Proteobacteria, Cyanobacteria and Planktomycetota being the dominant phyla. We identified pronounced spatial variability in community composition among five major Red Sea geographical regions with a third of all amplicon sequence variants being unique to the Southern Red Sea in contrast to a very homogenous distribution along the water column depth gradient. Our findings contribute to a deeper understanding of microbial ecology in the Red Sea and provide valuable insights into the factors governing pelagic particle-associated bacterioplankton communities in this basin.
Dataset DOI: 10.5061/dryad.vt4b8gv1k
Description of the data and file structure
Data for this manuscript was collected via filtering of seawater obtained by BottleNet sampling to retain environmental DNA, which then was extracted and eluted and sequenced, targeting the V3V4 region of the 16S SSU rRNA gene to describe bacterial communities. In addition, environmental parameters of the seawater were collected using a CTD.
Files and variables
File: clean_BPBN.csv
Variables
- asv: obtained ASVs from processing raw sequencing fastq files using the DADA2 algorithm.
- RSW 7 - RSW 282: distinct sample location, GPS, depth and environmental parameters provided in Supplementary File 1.
- Kingdom, Phylum, Class, Order, Family, Genus, Species: bacterial taxonomy ranks. (NA: no valid measurement)
Description: ASV-to-sample matrix
ZENODO files: NA in Supplementary File 1: Fluorometer did not measure valid data. NA in Supplementary File 5: Calculation was not possible.
Code/software
All code used for this publication can be accessed under https://github.com/lexscience/Fruehe2024-Bacterioplankton, together with all files needed to reproduce the data and output files generated for the publication.
Access information
Other publicly accessible locations of the data:
Data was derived from the following sources:
- None.