Adolescent alcohol exposure, pain, and synaptic function at BLA inputs onto prelimbic neurons
Data files
Apr 04, 2025 version files 175.66 KB
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aEPSCs.csv
6.15 KB
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AMPA-NMDA_Ratio.csv
10.48 KB
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AP_Threshold___AHP.csv
7.07 KB
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Carrageenan_average_mechanical_and_thermal.csv
4.21 KB
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Carrageenan_longitudinal_mechanical_and_thermal.csv
13.18 KB
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Excitation-Inhibition_Ratio.csv
8.02 KB
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Intrinsic_Excitability.csv
60.75 KB
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Longitudinal_mechanical_and_thermal_thresholds.csv
29.94 KB
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Membrane_properties.csv
9.80 KB
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PD109_Baseline_mechanical_and_thermal.csv
2.91 KB
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PD31-80_average_mechanical_and_thermal_threshold.csv
3.24 KB
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README.md
11.67 KB
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Rheobase.csv
2.58 KB
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Sag_Ratio.csv
5.66 KB
Abstract
This dataset covers mechanical and thermal sensitivity in PV-Cre rats (RRID:RRRC_00773) beginning in adolescence and continuing into adulthood. The rats were tested weekly for eight weeks. After the first test, half the rats began adolescent intermittent ethanol vapor exposure which continued for 4 weeks. Rats then underwent surgery to express channelrhodopsin in the BLA, and a viral cre-depedent mCherry tag in the prelimbic cortex. Retrobeads were injected in the ventrolateral periaqueductal gray. This allowed for slice electrophysiology recordings from prelimbic parvalbumin interneurons and prelimbic pyramidal neurons projecting to the ventrolateral periaqueductal gray. After recovering from the surgery, rats underwent a carrageenan inflammatory paw pain challenge. After assessing the effects of adolescent alcohol exposure in conjuction with the pain challenge on mechanical and thermal sensitivity, slice electrophysiology recordings were obtained from neurons in the prelimbic cortex. Parameters of interest for these recordings included excitatory/inhibitory balance, AMPA/NMDA ratio, and aEPSCs at BLA inputs onto PrL parvalbumin interneurons and periaqueductal gray projecting pyramidal neurons. Measures of intrinsic excitability were also obtained.
Dataset DOI: 10.5061/dryad.wwpzgmswd
Description of the data and file structure
These data include both behavioral assessments of mechanical and thermal pain sensitivity as well as data from slice electrophysiology recordings measuring synaptic function at basolateral amygdala inputs onto prelimbic parvalbumin positive interneurons and pyramidal neurons projecting from the prelimbic cortex to the ventrolateral periaqueductal gray. For the behavioral assessments, the electronic Von Frey and Hargreaves tests were used. Data were collected weekly for 8 weeks beginning at postnatal day 24 (PD 24) and continuing until PD 80. Half the rats began alcohol vapor exposure at PD 28. Rats underwent final reflexive pain assessments at approximately PD 109. This involved a baseline pain assessment followed by a carrageenan or saline (control) injection into the hindpaw. The effects of carrageenan on mechanical and thermal sensitivity were then measured at 2, 6, and 24 hours postinjection. Brain slices containing the prelimbic cortex were then prepared and electrophysiological recordings performed. Data from these recordings includes measures of intrinsic excitability, excitatory/inhibitory balance, AMPA/NMDA ratio, and asynchronous EPSCs.
Files and variables
File: Longitudinal_mechanical_and_thermal_thresholds.csv
Description: Longitudinal data on mechanical and thermal sensitivity in PV-Cre rats.
Variables
- Sex: Sex of the rat.
- Tx Group: Air (control) or AIE (adolescent intermittent ethanol)
- Age (Postnatal Day): Age of the rat at the time of the data collection.
- Withdrawal Threshold (gram-force): Amount of force required to elicit a paw withdrawal response. Reported in gram-force as measured using an electronic von Frey apparatus.
- Hargreaves Withdrawal Latency (s): Amount of time (in seconds) that elapsed before the hindpaw was withdraw during the Hargreaves test.
File: Membrane_properties.csv
Description: Membrane properties of prelimbic neurons.
Variables
- Sex: Sex of the rat.
- Challenge Group: Saline (control) or carrageenan (inflammatory paw pain).
- Tx Group: Air (control) or AIE (adolescent intermittent ethanol)
- Neuron Type: PRL-PAG (Prelimbic pyramidal neuron projecting to the ventrolateral periaqueductal gray) or PVIN (prelimbic parvalbumin positive interneuron).
- rm (MOhm): Membrane resistance in megaohms.
- vm (mV): Resting Membrane Potential in millivolts.
File: PD109_Baseline_mechanical_and_thermal.csv
Description: Baseline mechanical and thermal sensitivity prior to the carrageenan paw pain challenge. The test occurred at approximately PD109.
Variables
- Sex: Sex of the rat.
- Tx Group: Air (control) or AIE (adolescent intermittent ethanol)
- Withdrawal Threshold (gram-force): Amount of force required to elicit a paw withdrawal response. Reported in gram-force as measured using an electronic von Frey apparatus.
- Hargreaves Withdrawal Latency (s): Amount of time (in seconds) that elapsed before the hindpaw was withdraw during the Hargreaves test.
File: Sag_Ratio.csv
Description: Sag ratio of prelimbic neurons.
Variables
- Sex: Sex of the rat.
- Challenge Group: Saline (control) or carrageenan (inflammatory paw pain).
- Tx Group: Air (control) or AIE (adolescent intermittent ethanol)
- Neuron Type: PRL-PAG (Prelimbic pyramidal neuron projecting to the ventrolateral periaqueductal gray) or PVIN (prelimbic parvalbumin positive interneuron).
- sag ratio (%): Neuronal sag ratio.
File: PD31-80_average_mechanical_and_thermal_threshold.csv
Description: Averaged mechanical and thermal threshold for rats during and after adolescent alcohol vapor exposure.
Variables
- Sex: Sex of the rat.
- Tx Group: Air (control) or AIE (adolescent intermittent ethanol)
- Withdrawal Threshold (gram-force): Average amount of force required to elicit a paw withdrawal response. Reported in gram-force as measured using an electronic von Frey apparatus. The average was taken across values beginning at PD31 and continuing until PD80. See Longitudinal_mechanical_and_thermal_thresholds.csv for unaveraged values.
- Hargreaves Withdrawal Latency (s): Average amount of time (in seconds) that elapsed before the hindpaw was withdraw during the Hargreaves test. The average was taken across values beginning at PD31 and continuing until PD80. See Longitudinal_mechanical_and_thermal_thresholds.csv for unaveraged values.
File: Rheobase.csv
Description: Rheobase of prelimbic pyramidal neurons projecting to the ventrolateral periaqueductal gray.
Variables
- Sex: Sex of the rat.
- Challenge Group: Saline (control) or carrageenan (inflammatory paw pain).
- Tx Group: Air (control) or AIE (adolescent intermittent ethanol)
- Neuron Type: PRL-PAG (Prelimbic pyramidal neuron projecting to the ventrolateral periaqueductal gray) or PVIN (prelimbic parvalbumin positive interneuron).
- rheobase (pA): Neuronal rheobase.
File: AMPA-NMDA_Ratio.csv
Description: Monosynaptic optically evoked AMPA and NMDA currents at basolateral amygdala inputs onto prelimbic neurons.
Variables
- Sex: Sex of the rat.
- Challenge Group: Saline (control) or carrageenan (inflammatory paw pain).
- Tx Group: Air (control) or AIE (adolescent intermittent ethanol)
- Neuron Type: PRL-PAG (Prelimbic pyramidal neuron projecting to the ventrolateral periaqueductal gray) or PVIN (prelimbic parvalbumin positive interneuron).
- oAMPA_+40 (pA): Peak amplitude of the optically evoked AMPA current measured while holding the cell at +40 mV.
- oAMPA_-70 (pA): Peak amplitude of the optically evoked AMPA current measured while holding the cell at -70 mV.
- oNMDA (pA): Peak amplitude of the optically evoked NMDA current measured while holding the cell at +40 mV.
- AMPA_+40/NMDA ratio: Ratio of amplitude of the oAMPA (+40 mV) current to the NMDA current.
- AMPA_-70/NMDA ratio: Ratio of the amplitude of the oAMPA (-70 mV) current to the NMDA current.
- rectification index: AMPA rectification index computed as the ratio of the oAMPA (+40 mV) / oAMPA (-70 mV) peak amplitudes.
File: AP_Threshold___AHP.csv
Description: Action potential threshold and afterhyperpolarization of neurons in the prelimbic cortex.
Variables
- Sex: Sex of the rat.
- Challenge Group: Saline (control) or carrageenan (inflammatory paw pain).
- Tx Group: Air (control) or AIE (adolescent intermittent ethanol)
- Neuron Type: PRL-PAG (Prelimbic pyramidal neuron projecting to the ventrolateral periaqueductal gray) or PVIN (prelimbic parvalbumin positive interneuron).
- AP threshold (mV): Action potential threshold measured in millivolts.
- afterhyperpolarization (mV): Neuronal afterhyperpolarization measured in millivolts.
File: Carrageenan_average_mechanical_and_thermal.csv
Description: Average mechanical and thermal sensitivity of rats following a saline or carrageenan injection in the right hindpaw.
Variables
- Sex: Sex of the rat.
- Challenge Group: Saline (control) or carrageenan (inflammatory paw pain).
- Tx Group: Air (control) or AIE (adolescent intermittent ethanol)
- Withdrawal Threshold (% Baseline): Average withdrawal threshold expressed as a percentage of each rats baseline withdrawal force. The average was taken across the 2-, 6-, and 24-hr postinjection timepoints. See PD109_Baseline_mechanical_and_thermal.csv for baseline values. See Carrageenan_longitudinal_mechanical_and_thermal.csv for the values that were averaged.
- Hargreaves Withdrawal Latency (% Baseline): Average amount of time that elapsed before the hindpaw was withdraw during the Hargreaves test. The withdrawal latency is expressed as a percentage of the baseline withdrawal latency. The average was taken across the 2-, 6-, and 24-hr postinjection timepoints. See PD109_Baseline_mechanical_and_thermal.csv for baseline values. See Carrageenan_longitudinal_mechanical_and_thermal.csv for the values that were averaged.
File: Carrageenan_longitudinal_mechanical_and_thermal.csv
Description: Measurements of mechanical and thermal sensitivity taken at 2-, 6-, and 24-hr following an injection with saline or carrageenan in the right hindpaw.
Variables
- Sex: Sex of the rat.
- Challenge Group: Saline (control) or carrageenan (inflammatory paw pain).
- Tx Group: Air (control) or AIE (adolescent intermittent ethanol)
- Time (hr): Time postinjection.
- Withdrawal Threshold (% Baseline): The withdrawal threshold expressed as a percentage of the baseline value. See PD109_Baseline_mechanical_and_thermal.csv for baseline values. The withdrawal threshold was obtained using an electronic von Frey apparatus.
- Hargreaves Withdrawal Latency (% Baseline): The withdrawal latency expressed as a percentage of the baseline value. See PD109_Baseline_mechanical_and_thermal.csv for baseline values. The withdrawal latency was obtained using a Hargreaves apparatus.
File: Intrinsic_Excitability.csv
Description: Number of evoked action potentials elicited by increasing current injection steps. Recordings were obtained from prelimbic neurons.
Variables
- Sex: Sex of the rat.
- Challenge Group: Saline (control) or carrageenan (inflammatory paw pain).
- Tx Group: Air (control) or AIE (adolescent intermittent ethanol)
- Step (pA): Amount of current injected. The duration of current injection was 1 second.
- prl_pag: Number of action potentials generated by a prelimbic pyramidal neuron projecting to the ventrolateral periaqueductal gray in response to an injection of a known amount of current.
- pvin: Number of action potentials generated by a prelimbic parvalbumin positive interneuron in response to an injection of a known amount of current.
File: aEPSCs.csv
Description: Monosynaptic optically evoked asynchronous excitatory postsynaptic currents. Slice electrophysiology recordings were obtained from prelimbic neurons while optically stimulating the terminals of neurons projecting to the prelimbic cortex from the basolateral amygdala.
Variables
- Sex: Sex of the rat.
- Challenge Group: Saline (control) or carrageenan (inflammatory paw pain).
- Tx Group: Air (control) or AIE (adolescent intermittent ethanol)
- Neuron Type: PRL-PAG (Prelimbic pyramidal neuron projecting to the ventrolateral periaqueductal gray) or PVIN (prelimbic parvalbumin positive interneuron).
- peak (pA): Average peak amplitude of the aEPSCs.
- interevent interval (ms): Average interevent interval of the aEPSCs.
File: Excitation-Inhibition_Ratio.csv
Description: Polysynaptic optically evoked excitatory (recorded while holding the cell at +70 mV) and inhibitory (recorded while holding the cell at +10 mV) postsynaptic currents at prelimbic neurons. These currents were obtained by optically stimulating terminals from the basolateral amygdala.
Variables
- Sex: Sex of the rat.
- Challenge Group: Saline (control) or carrageenan (inflammatory paw pain).
- Tx Group: Air (control) or AIE (adolescent intermittent ethanol)
- Neuron Type: PRL-PAG (Prelimbic pyramidal neuron projecting to the ventrolateral periaqueductal gray) or PVIN (prelimbic parvalbumin positive interneuron).
- oEPSC (pA): Peak amplitude of the optically evoked excitatory postsynaptic current.
- oIPSC (pA): Peak amplitude of the optically evoked inhibitory postsynaptic current.
- E/I Ratio: Ratio of the oEPSC/oIPSC peak amplitudes.