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Targeted metabolomics data for dimethyl-ribityl lumazine (DMRL) and riboflavin in Mycobacterium tuberculosis and Mycolicibacterium smegmatis mutants of the riboflavin biosynthetic pathway

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Jul 01, 2025 version files 27.76 MB

Abstract

This dataset contains raw mutliple reaction monitoring (MRM) data for intermediate metabolites of the riboflavin biosynthetic pathway (RBP) in wild-type and riboflavin biosynthetic pathway gene deletion mutants of Mycobacterium tuberculosis and Mycolicibacterium smegmatis. These strains were generated to investigate the source of metabolic antigens recognized by mucosal-associated invariant T (MAIT) cells, which detect intermediates from the riboflavin biosynthetic pathway. To determine the impact of these gene deletions on the production of riboflavin and its precursors, we developed an MRM assay for DMRL and Riboflavin. This dataset provides evidence of how these disruption impact the biosynthesis of DMRL and Riboflavin which has enables a better understanding of the source of MR1 antigen in mycobacteria.