Data from: Role of Atg3, Atg5, and Atg12 in the crosstalk between apoptosis and autophagy in the posterior silk gland of Bombyx mori
Data files
Mar 17, 2025 version files 30.18 KB
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RAW_DATA_.xlsx
28.64 KB
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README.md
1.54 KB
Abstract
Autophagy is a cellular mechanism that enhances cell survival in response to various stressors, including nutrient deprivation; however, it also plays a pivotal role in the regulation of programmed cell death. This study examined the effects of autophagy-related genes Atg3, Atg5, and Atg12 on apoptosis and autophagy during the degeneration of the posterior silk gland in Bombyx mori, employing RNA interference techniques. Apoptosis-specific markers and autophagic processes were evaluated in both control and treatment groups. The knockdown of all three genes resulted in a significant reduction in autophagy, modifications in the apoptosis process, aberrant expression of p53, and impaired lysosomal function. It was determined that Atg3 is involved in the regulation of intracellular mitochondrial homeostasis. Following the silencing of Atg5, evidence was obtained indicating the gene's role in regulating lysosomal pH. Notably, the loss of Atg3 and Atg5 was associated with an increase in apoptotic markers, whereas the silencing of Atg12 inhibited apoptosis. Elevated levels of the p53 transcription factor following gene silencing suggested a potential interaction between these genes and p53. Our findings further underscore the importance of autophagy-mediated cell death, involving Atg3, Atg5, and Atg12, in the proper progression of degeneration in the posterior silk gland. A comprehensive understanding of the molecular mechanisms that mediate the interaction between apoptosis and autophagy is essential for elucidating their roles in both physiological and pathological contexts.
Role of Atg3, Atg5, and Atg12 in the crosstalk between apoptosis and autophagy in the posterior silk gland of Bombyx mori
https://doi.org/10.5061/dryad.zkh1893kv
Description of the data and file structure
File: RAW_DATA.xlsx
Description:
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The Figure 1A tab within the Excel file presents the data derived from quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis conducted on both control and treatment groups following the silencing of the Atg3, Atg5, Atg6, and Atg12 genes in Bombyx mori.
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The tab labeled Figure 2B tab in the Excel file presents data analyzed through quantitative reverse transcription polymerase chain reaction (qRT-PCR) from both the control and gene-silencing groups. This analysis aims to assess the expression levels of autophagy-related genes, specifically Atg3, Atg5, Atg6, Atg8, and Atg12.
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In the Excel file, Figure 3A tab contains data on the spectrophotometric analysis of acid phosphatase, a lysosomal enzyme, in the control and treatment groups.
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In the Excel file, Figure 4B tab labeled contains the data derived from the analysis of TUNEL images obtained from both the control and gene-silenced groups using ImageJ software.
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The Figure 5A tab in the Excel file presents the data acquired from the Western blot analysis of nuclear and cytoplasmic p53, cytosolic cytochrome c, and cleaved caspase-3 proteins in both control and gene-silenced groups.
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All the missing data given as n/a.