HIV efficiently infects T cells from the endometrium and remodels them to promote systemic viral spread
Data files
Apr 24, 2020 version files 3.62 GB
-
Donor1_Infected.fcs
241.08 MB
-
Donor1_PBMC_Infected.fcs
105.77 MB
-
Donor1_PBMC_Uninfected.fcs
92.17 MB
-
Donor1_Uninfected.fcs
98.46 MB
-
Donor10_Infected.fcs
29.44 MB
-
Donor10_Uninfected.fcs
18.46 MB
-
Donor10_YM155_Infected.fcs
46.15 MB
-
Donor10_YM155_Uninfected.fcs
98.75 MB
-
Donor11_Infected.fcs
60.87 MB
-
Donor11_Uninfected.fcs
47.24 MB
-
Donor11_YM155_Infected.fcs
57.56 MB
-
Donor11_YM155_Uninfected_c11_20191122_ET_YM155_01_0.fcs
48.99 MB
-
Donor2_Infected.fcs
93 MB
-
Donor2_PBMC_Infected.fcs
22.26 MB
-
Donor2_PBMC_Uninfected.fcs
14.64 MB
-
Donor2_Uninfected.fcs
65.54 MB
-
Donor3_Infected.fcs
362.48 MB
-
Donor3_PBMC_Infected.fcs
80.83 MB
-
Donor3_PBMC_Uninfected.fcs
53.81 MB
-
Donor3_Uninfected.fcs
242.08 MB
-
Donor4_Infected.fcs
331.27 MB
-
Donor4_PBMC_Infected.fcs
113.09 MB
-
Donor4_PBMC_Uninfected.fcs
58.32 MB
-
Donor4_Uninfected.fcs
311.80 MB
-
Donor5_Infected.fcs
66.13 MB
-
Donor5_Uninfected.fcs
49.36 MB
-
Donor6_Infected.fcs
68.60 MB
-
Donor6_Uninfected.fcs
40.64 MB
-
Donor7_Infected.fcs
95.30 MB
-
Donor7_Uninfected.fcs
47.56 MB
-
Donor8_Infected.fcs
147.03 MB
-
Donor8_Uninfected.fcs
109.73 MB
-
Donor9_Infected.fcs
39.68 MB
-
Donor9_Uninfected.fcs
37.39 MB
-
Donor9_YM155_Infected.fcs
180.19 MB
-
Donor9_YM155_Uninfected.fcs
42.11 MB
Abstract
The female reproductive tract (FRT) is the most common site of infection during HIV transmission to women, but viral remodeling complicates characterization of cells targeted for infection. Here, we report extensive phenotypic analyses of HIV-infected endometrial cells by CyTOF, and use a "nearest neighbor" bioinformatics approach to trace cells to their original pre-infection phenotypes. Like in blood, HIV preferentially targets memory CD4+ T cells in the endometrium, but these cells exhibit unique phenotypes and sustain much higher levels of infection. Genital cell remodeling by HIV includes downregulating TCR complex components and modulating chemokine receptor expression to promote dissemination of infected cells to lymphoid follicles. HIV also upregulates the anti-apoptotic protein BIRC5, which when blocked promotes death of infected endometrial cells. These results suggest that HIV remodels genital T cells to prolong viability and promote viral dissemination a
nd that interfering with these processes might reduce the likelihood of systemic viral spread.