Data from: Molecular signatures of host specificity linked to habitat specialization in Exaiptasia sea anemones.

Bellis ES, Edlund RB, Berrios HK, Lessios HA, Denver DR

Date Published: May 1, 2018

DOI: https://doi.org/10.5061/dryad.g8t9240

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Title Sample_collections
Downloaded 2 times
Description This file contains GPS coordinates (LAT/LON), species assignment codes (E. brasiliensis [Ebra] or E. pallida [Epal]), the dominant clade of Symbiodinium hosted by each anemone (B1, B2 or A), and other meta-information for each sample that was genotyped. E. pallida samples are identified as representatives of the global or Bocas-specific populations (or admixed if admixture proportions >35%). If the sample was identified as a clone of one or more other samples in the dataset, this is indicated in the CLONE column by a corresponding MLG identifier (e.g. "MLG5").
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Title Abundance
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Description Contains files necessary to recreate analyses of anemone abundance. Bocas_transects_individual_roots.csv contains abundance data for the three sites within the Bocas del Toro archipelago, where anemones were counted on mangrove roots from 30-m transects. Each row contains the number of anemones per root ("Abundance_root") for a different mangrove root in a particular 30-m transect ("Transect"). The collection site (Isla Colon/Cayo Roldan/Cayo Agua) is given in the "Population" column, as well as the GPS coordinates, taken at the start of the transect ("Lat"/"Lon"). The distance into the transect in meters is given in the "Distance_m" column. distances_bw_transects.csv: This file contains the shortest distance in meters (e.g. as the crow flies) between the start of one transect to the start of another, for transects that were conducted in continuous surveys.
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Title SymbiodiniumDensity
Downloaded 3 times
Description Protein.csv contains absorbance values from Pierce BCA assay determination of total protein (2 replicates per sample). Columns "Abs1" and "Abs2" are the raw absorbance values. "Vol_mL" is the total volume of the supernatant. Total protein is given in both micrograms ("TotalProtein_ug") and mg ("mg"). The date of the assay is given in the "Date" column, and different batches are indicated by the capital letters appended to the date. Algal_Counts.csv contains cell count/mitotic index data from hemocytometer counts. The R script algae.R uses these files to calculate algal cell density within each anemone. Note that we collected more anemones than we genotyped via 2bRAD, so these files contain data for more samples than are present in the Sample_collections.txt file.
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Title PopulationStructure
Downloaded 1 time
Description apal.5x.nc2.mac4.vcf contains vcf file of genotypes for all E. pallida individuals after clone removal, but before filtering for loci with a minimum allele frequency of 5%. pcadapt_analysis_final.R contains code to perform PCA analysis with pcadapt. This analysis is based on a SNP matrix (SNPmat0.05.txt) of 2577 SNPs that have a minimum allele frequency of 5%. The names of the loci are in locusNames_0.05.txt and information about the population designation for each sample is in pop_file.csv. Gene set enrichment analyses in ErmineJ used the Gene Annotation file bvpSNPS_erminej.txt and were based on the PC1 loadings in column 3 of PCloadings_final.txt. The file bvpSNPS_erminej.txt contains the AIPGENE models for all SNPs in apal.5x.nc2.mac4.vcf that were present in genic regions (5'UTR, CDS, intron or 3'UTR) as well as GO annotations from the Aiptasia genome v1.0. out.weir.fst is a file containing Weir and Cockerham FST values output from vcftools for E. pallida from the global lineage vs. the Bocas specific lineage. This was based on the 2577 biallelic SNPs with minimum allele frequency of 5%.
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Title Sequencing
Description Raw reads generated for 2bRAD-Seq libraries of 155 samples. Paired-end reads are available under NCBI BioProject accession PRJNA394157. Only R1 (forward) reads were used for analysis in the manuscript.
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When using this data, please cite the original publication:

Bellis ES, Edlund RB, Berrios HK, Lessios HA, Denver DR (2018) Molecular signatures of host specificity linked to habitat specialization in Exaiptasia sea anemones. Ecology and Evolution 8(11): 5413-5426. https://doi.org/10.1002/ece3.4058

Additionally, please cite the Dryad data package:

Bellis ES, Edlund RB, Berrios HK, Lessios HA, Denver DR (2018) Data from: Molecular signatures of host specificity linked to habitat specialization in Exaiptasia sea anemones. Dryad Digital Repository. https://doi.org/10.5061/dryad.g8t9240
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