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dc.contributor.author Tomina, Yusuke
dc.contributor.author Wagenaar, Daniel A
dc.date.accessioned 2017-10-10T13:04:23Z
dc.date.available 2017-10-10T13:04:23Z
dc.date.issued 2017-09-25
dc.identifier doi:10.5061/dryad.m20kh
dc.identifier.citation Tomina Y, Wagenaar DA (2017) A double-sided microscope to realize whole-ganglion imaging of membrane potential in the medicinal leech. eLife 6:e29839.
dc.identifier.uri http://hdl.handle.net/10255/dryad.151463
dc.description Studies of neuronal network emergence during sensory processing and motor control are greatly promoted by technologies that allow us to simultaneously record the membrane potential dynamics of a large population of neurons in single cell resolution. To achieve whole-brain recording with the ability to detect both small synaptic potentials and action potentials, we developed a voltage-sensitive dye (VSD) imaging technique based on a double-sided microscope that can image two sides of a nervous system simultaneously. We applied this system to the segmental ganglia of the medicinal leech Hirudo verbana. Double-sided VSD imaging enabled simultaneous recording of membrane potential events from almost all of the identifiable neurons. Using data obtained from double-sided VSD imaging we analyzed neuronal dynamics in both sensory processing and generation of behavior and constructed functional maps for identification of neurons contributing to these processes.
dc.relation.haspart doi:10.5061/dryad.m20kh/7
dc.relation.haspart doi:10.5061/dryad.m20kh/1
dc.relation.haspart doi:10.5061/dryad.m20kh/2
dc.relation.haspart doi:10.5061/dryad.m20kh/3
dc.relation.haspart doi:10.5061/dryad.m20kh/4
dc.relation.haspart doi:10.5061/dryad.m20kh/5
dc.relation.isreferencedby doi:10.7554/elife.29839
dc.title Data from: A double-sided microscope to realize whole-ganglion imaging of membrane potential in the medicinal leech
dc.type Article
dwc.ScientificName Hirudo verbana
dc.contributor.correspondingAuthor Tomina, Yusuke
prism.publicationName eLife
dryad.dansTransferDate 2018-05-20T07:10:21.052+0000
dryad.dansEditIRI https://easy.dans.knaw.nl/sword2/container/c10ca801-a1eb-4d8e-a0bc-40010ad3edd9
dryad.dansArchiveDate 2018-05-20T08:01:57.307+0000

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Title Supplementary
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Title functions
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Description All M-file codes were written in GNU Octave, version 4.0.0 (Free software licensed under the GNU General Public License; https://www.gnu.org/software/octave). You will first need to download octave functions of Wagenaar lab package (https://github.com/wagenadl/octave-wagenaarlab/releases/latest) and Qplot package (https://github.com/wagenadl/qplot/releases/latest) from from GitHub. Then, load packages of 'wagenaarlab', 'qplot', 'signal', 'statistics' before running a code. A manual of M-file functions for analyzing Vscope datasets (by Daniel A. Wagenaar) is available for understanding and using those functions ('vscope_functionNotes.txt' in the folder 'functions').
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