Show simple item record Gargiulo, Roberta Worswick, Gemma Arnold, Corinne Pike, Lindsay Cowan, Robyn S. Hardwick, Kate A. Chapman, Ted Fay, Michael F. 2019-05-15T15:57:12Z 2019-05-15T15:57:12Z 2019-05-15
dc.identifier doi:10.5061/dryad.q0ht491
dc.identifier.citation Gargiulo R, Worswick G, Arnold C, Pike L, Cowan RS, Hardwick KA, Chapman T, Fay MF (2019) Conservation of the threatened species Pulsatilla vulgaris Mill. (pasqueflower) is aided by reproductive system and polyploidy. Journal of Heredity.
dc.description Population loss due to habitat disturbance is a major concern in biodiversity conservation. Here we investigate the genetic causes of the demographic decline observed in English populations of Pulsatilla vulgaris and the consequences for conservation. Using ten nuclear microsatellite markers, we compare genetic variation in wild populations with restored and seed-regenerated populations (674 samples). Emergence of genetic structure and loss of allelic variation in natural populations is not as evident as expected from demographic trends. Restored populations show genetic variation comparable to their source populations and, in general, to the wild ones. Genetic homogeneity is observed in regeneration trials, although some alleles not captured in source populations are detected. We infer that polyploidy, longevity and clonal reproduction have provided P. vulgaris with the standing genetic variation necessary to make the species resilient to the effects of demographic decline, suggesting that the use of multiple sources for reintroduction may be beneficial to mimic natural gene flow and the availability of multiple allele copies typical of polyploid species.
dc.relation.haspart doi:10.5061/dryad.q0ht491/1
dc.relation.haspart doi:10.5061/dryad.q0ht491/2
dc.relation.haspart doi:10.5061/dryad.q0ht491/3
dc.relation.haspart doi:10.5061/dryad.q0ht491/4
dc.relation.haspart doi:10.5061/dryad.q0ht491/5
dc.relation.isreferencedby doi:10.1093/jhered/esz035
dc.subject clonal propagation
dc.subject in situ conservation
dc.subject nSSRs
dc.subject reintroduction
dc.subject seed bank
dc.subject population decline Subject area: Conservation genetics and biodiversity
dc.subject Population structure and phylogeography
dc.title Data from: Conservation of the threatened species Pulsatilla vulgaris Mill. (pasqueflower) is aided by reproductive system and polyploidy
dc.type Article
dwc.ScientificName Pulsatilla vulgaris
dc.contributor.correspondingAuthor Gargiulo, Roberta
prism.publicationName Journal of Heredity
dryad.dashTransferDate 2019-07-20T00:43:33.528+0000

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Title Microsatellite dataset
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Title Recoded dataset
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Description Dataset recoded in Polysat (function recodeAllopoly)
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Title Alleles in derived populations
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Description Alleles in restored/regenerated populations not occurring in wild-origin population samples. Occurrences are expressed by their De Silva frequency.
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Title Allelic capture
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Description Alleles occurring in wild origin populations which were also captured in restored/regenerated populations. Occurrences are expressed by their De Silva frequencies.
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Title De Silva Frequencies
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