Data from: Stem cell differentiation trajectories in Hydra resolved at single-cell resolution
Data files
Jul 25, 2019 version files 18.18 GB
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Candidate_Regulators.zip
377.83 MB
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Drop_seq_references.zip
253.26 MB
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Drop-seq_pipeline_initialQC.tar.gz
4.64 MB
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Hydra_Seurat_Ecto.rds
510.32 MB
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Hydra_Seurat_Endo_lineage_plot.rds
1.11 GB
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Hydra_Seurat_Endo.rds
1.12 GB
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Hydra_Seurat_IC.rds
3.06 GB
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Hydra_Seurat_Neurons.rds
689.75 MB
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Hydra_Seurat_NMF_regulators_analyses.zip
39.90 MB
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Hydra_Seurat_Whole_Genome.rds
3.36 GB
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Hydra_Seurat_Whole_Transcriptome.rds
6.61 GB
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Hydra_URD_analysis_objects.zip
781.56 MB
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Hydra_URD_analysis.zip
15.44 MB
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Hydra_URD_cluster_results.zip
134.73 MB
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nmf.zip
27.03 MB
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README_for_Candidate_Regulators.txt
4.29 KB
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README_for_Drop-seq_pipeline_initialQC.tar.txt
680 B
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README_for_Hydra_URD_analysis.txt
1.26 KB
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README_for_Hydra_URD_cluster_results.txt
2.52 KB
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README_for_nmf.txt
1.13 KB
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README_for_transcriptome_aepLRv2.tar.txt
2.63 KB
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transcriptome_aepLRv2.tar.gz
92.87 MB
Abstract
The adult Hydra polyp continually renews all of its cells using three separate stem cell populations, but the genetic pathways enabling this homeostatic tissue maintenance are not well understood. We sequenced 24,985 Hydra single-cell transcriptomes and identified the molecular signatures of a broad spectrum of cell states, from stem cells to terminally differentiated cells. We constructed differentiation trajectories for each cell lineage and identified gene modules and putative regulators expressed along these trajectories, thus creating a comprehensive molecular map of all developmental lineages in the adult animal. In addition, we built a gene expression map of the Hydra nervous system. Our work constitutes a resource for addressing questions regarding the evolution of metazoan developmental processes and nervous system function.